Propolis modulates vitronectin, laminin, and heparan sulfate/heparin expression during experimental burn healing

Olczyk, Paweł; Komosińska-Vassev, Katarzyna; Winsz-Szczotka, Katarzyna; Koźma, Ewa M; Wisowski, Grzegorz; Stojko, Jerzy; Klimek, Katarzyna; Olczyk, Krystyna

doi:10.1631/jzus.b1100310

Cited by 30 publications

(34 citation statements)

References 52 publications

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“…Despite therapeutic activity of the drug, it should also quench radical activity. Therefore European propolis (including Polish origin epitherapeutic agent), a natural plant resin that is produced by honey bees, seems to be an optimal agent for the treatment of thermal injuries due to its antimicrobial, anti-inflammatory, antitumor, immunomodulatory, and last but not least antioxidant activity [7–10]. Beneficial biological effect of propolis has been widely used in dermatology for injuries healing, thermal damage and external ulcers therapy, healing time reduction, wound contraction increase, and tissue repair acceleration [11].…”

Section: Introductionmentioning

confidence: 99%

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Positive Effect of Propolis on Free Radicals in Burn Wounds

Olczyk

Ramos

Komosińska-Vassev

et al. 2013

Evidence-Based Complementary and Alternative Medicine

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Concentration and properties of free radicals in the burn wounds treated with propolis were examined by the use of electron paramagnetic resonance spectroscopy. Magnetic spin-spin interactions and complex free radicals structures in wound beds were studied. The results were compared to those obtained for silver sulphadiazine used as a standard pharmaceutical agent. The changes of free radicals in the matrix of injury with time of exposition on these substances were tested. The aim of this study was to check the hypothesis about the best influence of propolis on the burn wounds healing. It was confirmed that a relatively lower concentration of free radicals exists in the burn wounds treated with propolis. The homogeneously broadened spectra and a complex free radical system characterize the tested tissue samples. The fastening of spin-lattice relaxation processes in the matrix of injury after treatment with propolis and silver sulphadiazine was observed. Practical usefulness of electron paramagnetic resonance spectroscopy in alternative medicine was proved.

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Section: Introductionmentioning

confidence: 99%

“…In contrary to the last mentioned natural agent, silver sulphadiazine conventionally used for topical burn therapy, effective in controlling the infection of damaged skin, may lead to prolongation of the wound reepithelization process [10, 14–16]. …”

Section: Introductionmentioning

confidence: 99%

Positive Effect of Propolis on Free Radicals in Burn Wounds

Olczyk

Ramos

Komosińska-Vassev

et al. 2013

Evidence-Based Complementary and Alternative Medicine

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“…The expression of HS/H constituting a small amount of wound matrix total GAGs [6] was precisely discussed in our previous work [17]. As can be seen from Figure 2(a) the total amount of GAGs increased in the wound bed treated with propolis, AgSD, NaCl, and propolis vehicle.…”

Section: Resultsmentioning

confidence: 83%

Propolis Induces Chondroitin/Dermatan Sulphate and Hyaluronic Acid Accumulation in the Skin of Burned Wound

Olczyk

Komosińska-Vassev

Winsz-Szczotka

et al. 2013

Evidence-Based Complementary and Alternative Medicine

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Changes in extracellular matrix glycosaminoglycans during the wound repair allowed us to apply the burn model in which therapeutic efficacy of propolis and silver sulfadiazine was compared. Burns were inflicted on four pigs. Glycosaminoglycans isolated from healthy and burned skin were quantified using a hexuronic acid assay, electrophoretic fractionation, and densitometric analyses. Using the reverse-phase HPLC the profile of sulfated disaccharides released by chondroitinase ABC from chondroitin/dermatan sulfates was estimated. Chondroitin/dermatan sulfates and hyaluronic acid were found in all samples. Propolis stimulated significant changes in the content of particular glycosaminoglycan types during burn healing. Glycosaminoglycans alterations after silver sulfadiazine application were less expressed. Propolis maintained high contribution of 4-O-sulfated disaccharides to chondroitin/dermatan sulfates structure and low level of 6-O-sulfated ones throughout the observed period of healing. Propolis led to preservation of significant contribution of disulfated disaccharides especially 2,4-O-disulfated ones to chondroitin sulfates/dermatan sulfates structure throughout the observed period of healing. Our findings demonstrate that propolis accelerates the burned tissue repair by stimulation of the wound bed glycosaminoglycan accumulation needed for granulation, tissue growth, and wound closure. Moreover, propolis accelerates chondroitin/dermatan sulfates structure modification responsible for binding growth factors playing the crucial role in the tissue repair.

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“…According to the results of our study, it can also be concluded that propolis skin ointment appears to have beneficial effects on healing venous ulcers. Propolis has been shown to stimulate various enzyme systems, cell metabolism, circulation, and collagen formation as well as improve the healing of burn wounds [14]. In the study published by Kurson [15], sixty-four patients with tibial skin ulcers, aged from 23 to 98 years old, were treated using propolis extract in an ointment form.…”

Section: Discussionmentioning

confidence: 99%

Topical Treatment of Nonhealing Venous Leg Ulcer with Propolis Ointment

Kucharzewski

Kózka²,

Urbanek

2013

Evidence-Based Complementary and Alternative Medicine

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An investigation of effectiveness of topical treatment of nonhealing chronic venous leg ulcers with propolis ointment was conducted. 56 patients were included in the study and randomized into two groups. In group 1, there were 28 patients (ulceration area: 6.9–9.78 cm2) treated by means of topical propolis ointment application and short stretch bandage compression. In group 2, there were 29 patients (ulceration area: 7.2–9.4 cm2) treated by means of Unna boot leg compression without topical propolis treatment. In the study, the efficacy of both treatment methods in patients with resistive venous leg ulcers was compared. The ulceration of patients from group 1 healed completely after 6 weeks of therapy in all cases. In all patients from group 2, the process of healing was longer but successfully completed after 16 weeks of the therapy. We found that an adjunctive propolis ointment treatment increases the efficacy of the short stretch bandage compression stocking, and this combined treatment is more effective than Unna's boot compression alone.

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Propolis modulates vitronectin, laminin, and heparan sulfate/heparin expression during experimental burn healing

Abstract: The beneficial effects of propolis on experimental wounds make it a potential apitherapeutic agent in topical burn management.

Cited by 30 publications

References 52 publications

Positive Effect of Propolis on Free Radicals in Burn Wounds

Positive Effect of Propolis on Free Radicals in Burn Wounds

Propolis Induces Chondroitin/Dermatan Sulphate and Hyaluronic Acid Accumulation in the Skin of Burned Wound

Topical Treatment of Nonhealing Venous Leg Ulcer with Propolis Ointment

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