Proportion of Helicobacter pylori Among Dyspeptic Patients Detected by Molecular Methods in a Teaching Hospital in Sri Lanka

Ubhayawardana, D. L. N. L.; Weerasekera, Manjula; Weerasekera, Deepaka; Gunasekera, T.D.C.P.; Fernando, S.S.N.

doi:10.17795/ijep29796

Cited by 3 publications

(4 citation statements)

References 13 publications

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“…One biopsy was used for PCR targeting the glmM gene to identify H. pylori infection. DNA extraction from gastric biopsies and PCR was performed as mentioned in Ubhayawardana et al 2015 [6].…”

Section: Methodsmentioning

confidence: 99%

“…H. pylori has been classi ed as a class I carcinogen for humans by the International Agency for Research on Cancer (IARC) monograph committee in 1994. Prevalence of H. pylori infection is an enigma in Sri Lanka ranging from 3 to 70% [4,5,6]. Further resistance to clarithromycin is a problem [7] which results in eradication failure with triple therapy [6,8].…”

Section: Introductionmentioning

confidence: 99%

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Helicobacter pylori infection and chronic gastritis; use of the Updated Sydney System in histopathological evaluation of gastritis

Ubhayawardana¹,

Weerasekera²,

Samarasinghe³

et al. 2019

Preprint

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Objective Helicobacter pylori is a major cause for chronic gastritis and further it is associated with development of peptic ulcer disease and gastric cancer. Therefore, the objective of this study was to classify gastritis according to the updated Sydney system guidelines and find the association of H. pylori with each of graded variable. Number of 152 dyspeptic patients who underwent upper gastro-intestinal endoscopy at a Teaching Hospital were enrolled. Of the 2 biopsies collected one was used for PCR to detect H. pylori. The other biopsy was fixed in formalin followed by paraffin embedding and stained with H&E stain. Gastritis was classified microscopically according to the updated Sydney system. Results : Gastritis was reported over a wide age group ranging from 18-84 years with a mean age of 51 years. Based on histological findings, 12% of patients were diagnosed as H. pylori associated chronic active gastritis. There was no significant association between each graded variable and H. pylori positivity. Of the 152 dyspeptic patients 34 were positive by PCR for H. pylori infection. All the dyspeptic patients with H. pylori infection had chronic active gastritis, suggesting an etiologic role of the bacterium in the histologic lesion.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Helicobacter pylori infection and chronic gastritis; use of the Updated Sydney System in histopathological evaluation of gastritis

Ubhayawardana¹,

Weerasekera²,

Samarasinghe³

et al. 2019

Preprint

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“…35 Recent studies conducted in Sri Lanka have reported a lower proportion of H. pylori among dyspeptic patients. 35,36,37 However, there is limited data available on the gastric microbial diversity and related complications such as dysplasia, gastric cancer and the effects on both health and disease in Sri Lanka.…”

Section: Diversity Of Gastric Microbiotamentioning

confidence: 99%

Human stomach microbiota: Effects on health and disease

Ubhayawardana

Fernando

Gunasekara³

et al. 2021

Sri Lankan J Infec Dis

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The gut microbiota is a complex ecological community, consisting of trillions of microbes which include bacteria, viruses, fungi and protozoa. The stomach was previously considered as a sterile site uninhabited by microbes due to its hostile environmental conditions. Breaking this concept, Helicobacter pylori was the first pathogen reported to inhabit the stomach. Recent studies have suggested that the stomach harbours transient as well as certain commensal bacterial and fungal species. The five major microbial phyla in the stomach have been identified as Firmicutes, Bacteroidetes, Actinobacteria, Fusobacteria and Proteobacteria.The composition of gastric microbiota is dynamic and is affected by several factors. These include age group, dietary habits, medication use, inflammation of gastric mucosa and H. pylori colonization. Further, the role of host genetics has recently been studied in maintaining the stomach microbiota. Mutations in host genes may affect the host's immune response towards commensal bacteria and reduce their number and diversity.The essential multiple roles of gut microorganisms include maintaining homeostasis in the gut, contributing to immune function and extraction of nutrients and energy from our diets. Loss of the normal balance between the gut microbiota and host has been associated with several abnormal conditions and disorders such as obesity, malnutrition, inflammatory bowel diseases (IBD), neurological disorders, and cancer. In the stomach, the interaction between H. pylori and the gastric microbiota can also influence gastric disease progression. Further studies should focus on addressing the role of gastric dysbiosis in health and disease. Identifying gastric microbiota is essential to understand how the gut microbiota and H. pylori affect health and disease.

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“…The role of TNF‐α polymorphisms in severity of H. pylori‐ associated disease has not been studied in Sri Lanka. Previous histological studies done in Sri Lanka have suggested that most H. pylori‐ infected patients have mild chronic gastritis and that development of gastric atrophy is not a significant problem. Multiple factors can contribute to a shift from mild to atrophic gastritis; however, host genetic factors can play a major role.…”

mentioning

confidence: 98%

Association of tumor necrosis factor alpha gene polymorphisms with Helicobacter pylori infection in dyspeptic patients in Sri Lanka

Arachchi

Weerasekera

Senevirathna

et al. 2018

Microbiology and Immunology

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Single nucleotide polymorphisms present on the promoter sequence of the TNF-α gene may affect production of TNF-α, a pro-inflammatory cytokine, during immune responses. The presence of TNF-α polymorphisms is also reportedly associated with more severe manifestations of Helicobacter pylori infection. However, the frequency of TNF-α polymorphisms and the associated disease severity vary between different patient groups. In this study, gastric biopsies and blood specimens were collected from 138 patients with dyspepsia undergoing routine upper gastrointestinal endoscopy. Our institution's Ethics Review Committee approved the study and written informed consent was obtained from all participants. The presence of H. pylori was confirmed histologically in all patients. The frequency of TNF-α polymorphisms in the study cohort was investigated using PCR-restriction fragment length polymorphism and expression of serum TNF-α quantitated using a commercial ELISA assay. The proportions of selected TNF-α polymorphisms (TNF-α -238, -308 and -863) were similar in H. pylori-positive and -negative patients. Homozygous mutations of TNF-α polymorphisms were rarely detected in the study group. There was a significant difference in TNF-α concentrations between patients with mild chronic gastritis and TNF-α -308 GG genotype and patients with moderate to severe chronic gastritis (P = 0.008). It was not possible to identify an association between these genotypes and disease severity because of the low frequency of heterozygous and homozygous mutated genes in Sri Lankan patients with dyspepsia.

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Proportion of Helicobacter pylori Among Dyspeptic Patients Detected by Molecular Methods in a Teaching Hospital in Sri Lanka

Cited by 3 publications

References 13 publications

Helicobacter pylori infection and chronic gastritis; use of the Updated Sydney System in histopathological evaluation of gastritis

Helicobacter pylori infection and chronic gastritis; use of the Updated Sydney System in histopathological evaluation of gastritis

Human stomach microbiota: Effects on health and disease

Association of tumor necrosis factor alpha gene polymorphisms with Helicobacter pylori infection in dyspeptic patients in Sri Lanka

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