This study aims to improve soldiers' mission performance and protect them from safety accidents by establishing an optimal sizing system that considers the fit of the tactical gloves and the efficiency of production and supply. First, the wearing condition of tactical gloves was investigated through in-depth interviews and surveys. The optimal glove fit and loss coefficient ratio was then analyzed through a glove size selection experiment. Finally, the sizing system was optimized and verified by comparing the coverage rate to the current sizing system. Results indicated that the empirically derived loss coefficient ratio was 0.075 and the optimal sizing system for tactical gloves was S-hand length: 168, hand width: 81, M-hand length: 177, hand width: 83, L-hand length: 184, hand width: 86, XL-hand length: 191, hand width: 89, respectively. The coverage rate of the optimal sizing system proposed in this study was 86.4%, showing an improvement of approximately 21.1% compared to the current sizing system (65.3%). In conclusion, the optimal sizing system for tactical gloves proposed in this study can be a realistic solution, as it improved the coverage rate by 21.1% without incurring additional costs for production or hindering the efficiency of supply.