In this research, a generalized tool life modelling for considering non-stationary cutting conditions was developed . In particular, for the first time in literature, the model was conceived for predicting the life of the tool when spindle speed variation SSV, one of the most effective techniques for suppressing regenerative chatter vibrations, is used. The proposed formulation takes into account the main cutting parameters and the parameters associated to the SSV. A dedicated experimental campaign of turning tests was executed and the data were used for modelling purposes. The model validation was carried out performing additional tool life tests. According to the analyzed technological scenario, it was found that the generalized formulation can be used for predicting the tool life both at constant spindle machining CSM and adopting SSV with the maximum estimating error of 6%.