Proposal of Oscillochloridaceae fam. nov. on the basis of a phylogenetic analysis of the filamentous anoxygenic phototrophic bacteria, and emended description of Oscillochloris and Oscillochloris trichoides in comparison with further new isolates.

Abstract

The nucleotide sequences of the genes of 16S rRNAs were determined for the type strain Oscillochloris trichoides DG-6 T and three new strains of Oscillochloris-like mesophilic filamentous green bacteria. Two major clusters have been found within the family Chloroflexaceae by phylogenetic analysis : one cluster includes thermophilic species of Chloroflexus and the second includes mesophilic strains of Oscillochloris. The degree of relatedness of these clusters was below an intergeneric level, having only 825-865 % of 16S rDNA sequence similarity. These phylogenetic data correlate well with the significant physiological, biochemical and chemotaxonomical differences between members of both groups. Therefore, the Oscillochloris and Chloroflexus clusters should be considered as two separate families. The description of the new family, Oscillochloridaceae fam. nov., and emended descriptions of the genus Oscillochloris and the species Oscillochloris trichoides are presented.

Roseiflexus castenholzii gen. nov., sp. nov., a thermophilic, filamentous, photosynthetic bacterium that lacks chlorosomes.

“…In addition, absorption maxima at 479 and Symbols : j, positive ; k, negative ; , not determined. Data were taken from the following references : Chloroflexus spp., Pierson & Castenholz (1974) and Hanada et al (1995) ; O. trichoides, Keppen et al (2000) ; H. oregonensis, Pierson et al (1984Pierson et al ( , 1985. …”

“…This photosynthetic group includes some other genera : Oscillochloris (Keppen et al, 1994(Keppen et al, , 2000, Chloronema (Dubinina & Gorlenko, 1975) and Heliothrix (Pierson et al, 1985). In addition, several Chloroflexus-like bacteria have been observed in marine environments (Pierson et al, 1994).…”

Anaerolinea thermophila gen. nov., sp. nov. and Caldilinea aerophila gen. nov., sp. nov., novel filamentous thermophiles that represent a previously uncultured lineage of the domain Bacteria at the subphylum level

“…aggregans. Species of the genus Oscillochloris (Keppen et al, 1993(Keppen et al, , 1994(Keppen et al, , 2000Gorlenko & Pivovarova, 1977): Osc. chrysea (type species), Osc.…”

Reconstruction of bacteriochlorophyll biosynthesis pathways in the filamentous anoxygenic phototrophic bacterium Oscillochloris trichoides DG-6 and evolution of anoxygenic phototrophs of the order Chloroflexales

“…Autotrophic CO 2 assimilation by the representatives of family Chloroflexaceae (Chloroflexus aurantiacus) is performed through the 3-hydroxypropionate cycle (Herter et al, 2002;Zarzycki et al, 2009). The representatives of the family Oscillochloridaceae are able to grow as photo-organotrophs and photolithotrophs, and they are strict anaerobes (Keppen et al, 1994(Keppen et al, , 2000. Autotrophic CO 2 assimilation by the representatives of family Oscillochloridaceae is performed through the reductive pentose phosphate cycle (Berg et al, 2005;Ivanovsky et al, 1999;Turova et al, 2006).…”