2020
DOI: 10.1016/j.jmoldx.2020.07.001
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Proposal of RT-PCR–Based Mass Population Screening for Severe Acute Respiratory Syndrome Coronavirus 2 (Coronavirus Disease 2019)

Abstract: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) testing has lagged in many countries because of test kit shortages and analytical process bottlenecks. This study investigated the feasibility and accuracy of a sample pooling approach for wide-scale population screening for coronavirus disease 2019. A total of 940 nasopharyngeal swab samples (934 negative and 6 positive) previously tested for SARS-CoV-2 were deidentified and assigned random numbers for analysis, and 94 pools of 10 samples each were … Show more

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Cited by 28 publications
(22 citation statements)
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“… 7 , 8 This dilutes individual samples of the pools, and therefore might reduce the sensitivity of the RT-PCR, 7 , 9 especially in samples with low viral loads. 10 In our strategy, we combined two to seven individual swabs in one tube at the time of collection, and we observed no difference in the obtained Ct values between the pooled and individual NTS samples.…”
mentioning
confidence: 76%
“… 7 , 8 This dilutes individual samples of the pools, and therefore might reduce the sensitivity of the RT-PCR, 7 , 9 especially in samples with low viral loads. 10 In our strategy, we combined two to seven individual swabs in one tube at the time of collection, and we observed no difference in the obtained Ct values between the pooled and individual NTS samples.…”
mentioning
confidence: 76%
“…PerkinElmer's RT-PCR kit recently received US FDA EUA approval, and has a limit of detection of <20 copies ml −1 of SARS-CoV-2 (ref. 114 ). In another study, viability of pooling clinical naso-or oropharyngeal swabs during extraction of nucleic acid was seen without reducing the sensitivity of RT-PCR 115 .…”
Section: Discussionmentioning
confidence: 99%
“…Positivity rates would need to be adjusted with pooling numbers to balance the number of retests required. 39 , 40 …”
Section: Discussionmentioning
confidence: 99%