Propoxur-induced oxidative DNA damage in human peripheral blood mononuclear cells: protective effects of curcumin and α-tocopherol

Abstract

The present study enumerates the attenuating effects of curcumin and α-tocopherol against propoxur induced oxidative DNA damage in human peripheral blood mononuclear cells (PBMC). Cultured cells were isolated from peripheral blood of healthy volunteers, and were exposed to varying concentrations of propoxur (0-21 μg/ml) for 6, 12, and 24 h, and in combination with curcumin (9.2 μg/ml) or α-tocopherol (4.3 μg/ml) or both. Cytotoxic effect of propoxur was examined by MTT assay. The role of oxidative stress beneath the cytotoxicity of propoxur was evaluated by the measurement of reduced glutathione (GSH), malondialdehyde (MDA) and 8-hydroxy-2'-deoxyguanosine (8-OH-dG) levels in cell lysate. A concentration-dependent cell death, depletion of GSH, an increase in the level of both MDA and 8-OH-dG were observed. Co-treatment with curcumin or α-tocopherol significantly attenuates depleted GSH, decrease in MDA and 8-OH-dG levels in propoxur exposed cells (p < 0.05). The results of the present study provide experimental evidence of involvement of oxidative stress in propoxur-mediated genotoxicity in human PBMC and highlight the antioxidant role of curcumin and α-tocopherol following propoxur exposure.

Curcumin exerts its antioxidant and neuroprotective effects against aluminum-induced oxidative stress and neurotoxicity in male albino rats

“…These findings are consistent with those of Kar et al (2019), who said that curcumin administration resulted in a significant reduction in apoptotic, genotoxic, and oxidative stress-related indicators (Kumar, Sasmal, & Sharma, 2018;Nariya et al, 2018). According to Ahmed, Goel, and Banerjee (2018), curcumin may also protect DNA from damage by decreasing oxidative stress and apoptosis.…”

Antioxidant Effects of Vitamins C, E and Provitamin A Compounds as Monitored by Use of Biochemical Oxidative Indicators Linked to Atherosclerosis and Carcinogenesis

“…These findings agree with another human trial [66] in which 23 healthy subjects (after 2 weeks of washout) were supplemented daily with 40 mg lycopene (weeks 3 and 4), 22.3 mg βcarotene and 15.7mg α-carotene (weeks 5 and 6), and 11.3 mg lutein (weeks 7 and 8) This intervention has resulted into a significantly decreased DNA damage in lymphocytes. Kiokias & Gordon (2003) [61] conducted a double-blind, placebo-controlled cross over study with 30 healthy subjects.…”

“…Following a dietary supplementation with 30 mg carotenoid mixture /day (,-carotenes, lycopene, paprika, lutein, bixin, total amount) they reported a significant effect against production of urinary 8-OHdG estimated by use of ELISA test. Zou and co-authors (2014) [66] also highlighted that there could be a synergistic effect when carotenoids are mixed together because various compounds tend to arrange in different sites of membranes and lipoproteins, adding an enhanced protection against oxidative damage. More recently, a crosssectional study [60] supplemented 296 healthy middle-aged men with a mixture of all 3 provitamin A compounds and two more carotenoids (lycopene and lutein).…”

A symbiotic gut bacterium enhances Aedes albopictus resistance to insecticide

“…This also means that this may be a slow biological process, which explains why there was no difference in knockdown rate, but the survival rate became prominent after 24h of recovery. We did not study S. oryzae's ability to degrade other types of insecticides, such as propoxur due to its strong cytotoxicity causing oxidative damage to mammals [53]. One of the most significant targets of oxidative damage is DNA that can promote apoptosis [54].…”