ProSAAS peptide of the granin protein family in biochemical diagnostics of pheochromocytoma

Abstract

methods PatientsTwenty-three patients (13 women aged 36-87 years, 10 men aged 41-81 years) with pheochromocytoma were recruited. Eighteen cases of benign pheochromocytoma and five cases of malignant form were diagnosed. The following were obtained from all patients: clinical history, basic laboratory tests and specialist biochemical tests (plasma metanephrine and normetanephrine levels, serum CgA concentration), abdominal imaging [computed tomography (CT) and/or magnetic resonance imaging (MRI)]. Also, in selected patients scintigraphy [ 123 I-metaiodobenzylguanidine scintigraphy ( 123 I-MIBG) and/or somatostatin receptor scintigraphy (SRS)] was also done. After pharmacological preparation, all patients were referred for surgical treatment (laparoscopic or classic surgery). The diagnosis of pheochromocytoma was confirmed by histopathological examination of tumour specimens and by immunohistochemical assays (CgA and synaptophysin). The control group consisted of 35 healthy volunteers (blood donors): 17 women aged 19-48 years and 18 men aged 20-52 years. In this group, plasma levels of metanephrine and normetanephrine as well as serum CgA levels were determined. In both groups of subjects (patients with pheochromocytoma and healthy participants) the plasma concentration of the proSAAS peptide was determined.

Chromogranin A as a diagnostic marker of pheochromocytoma and paraganglioma: A systematic review and meta‐analysis

“…After a thorough examination, we analyzed the remaining 81 articles, which were deemed possibly pertinent, and excluded 68 studies that did not meet our criteria. Finally, 13 articles, which encompassed a total of 1470 patients, were incorporated into this meta-analysis [11][12][13][14][15][16][17][18][19][20][21][22][23] (Figure 1).…”

“…In a prior study by Liu et al., it was observed that combined detection of MNs and CgA maintained high sensitivity but could potentially result in reduced specificity 18 . However, Glinicki et al 22 . further emphasized the potential of CgA by revealing that its combination with either MN or NMN could significantly enhance both sensitivity and specificity in PPGL diagnosis.…”

“…In a prior study by Liu et al, it was observed that combined detection of MNs and CgA maintained high sensitivity but could potentially result in reduced specificity. 18 However, Glinicki et al 22 further emphasized the potential of CgA by revealing that its combination with either MN or NMN could significantly enhance both sensitivity and specificity in PPGL diagnosis. Additionally, the determination of CgA proves beneficial for patients with plasma MNs negativity (normal), such as those with malignant tumors, although this scenario is infrequent.…”