Prospecting Filamentous Fungi for Amylase Production: Standardization of Aspergillus japonicus Culture Conditions

Abstract

Aims: Prospecting different filamentous fungi for high production of amylases in standard conditions for future application in biotechnology industries. Methodology: Samples were collected in different field areas in the state of Bahia, Brazil, for isolating filamentous fungi. Fungi were grown in Petri dishes in a culture medium containing 4% Quaker® oatmeal and 2% bacteriological agar. Fungi screening was carried out in liquid medium containing 1% starch at 30°C and pH6.0 under static conditions for 4 days. Proteins and enzyme activities were determined by Bradford and DNS methods, respectively. A submerged fermentation was performed with different liquid media in order to obtain the best growth composition and enzyme production for the selected fungus. Several conditions such as time course of inactivation, pH, temperature, carbon and nitrogen sources were determined in the culture medium with the aim of

Sugarcane bagasse as a source of carbon for enzyme production by filamentous fungi1

“…The characteristics of the β-glucosidase produced by A. japonicus have been previously assessed for a potential use in biomass saccharification (Korotkova et al 2009). Pasin et al (2014) reported high levels of amylase activity in A. japonicus when cultivated in different agro-industrial residues. The presence of this activity in the cultures might be due to the fact that amylases are constitutive enzymes, which are generally located in the fungal cell walls (Benassi et al 2012).…”

Avaliação Microbiológica De Uma Cerveja Tipo Pilsen Com Adição De Chá Verde Na Etapa De Maturação

“…O aumento de infecções microbianas e a resistência dos patógenos a medicamentos disponíveis no mercado tem despertado grande interesse na pesquisa por novas drogas de origem vegetal (JENSEN et al, 2015 (UMAR et al, 2016). Em concordância com os resultados desta pesquisa, autores citam que o extrato etanólico das cascas dos frutos de romã é capaz de inibir o crescimento da espécie Staphylococcus aureus (MOREIRA et al, 2014).…”

Análise Da Temperatura De Crescimento De Fungos Filamentosos Produtores De Amilases

“…O Brasil é um dos países com maior diversidade de organismos vivos, incluindo os fungos filamentosos que são encontrados em abundância nos distintos habitats (PEREIRA, 2017). A prospecção de fungos filamentos vem se destacando, visto que estes representam uma rica fonte em recursos genéticos para aplicação em inúmeros processos e produtos biotecnológicos (PASIN, 2014). O uso das enzimas se destaca entre os metabólitos fúngicos explorados.…”