Prospecting of essential oils in combination with florfenicol against motile Aeromonas isolated from tambaqui (Colossoma macropomum)

Silva, Ana Maria Souza da; Chagas, Edsandra Campos; Chaves, F. C. M.; Sebastião, Fernanda de Alexandre

doi:10.1007/s00203-022-03015-4

Cited by 2 publications

(2 citation statements)

References 47 publications

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“…The access to the botanical material was registered on the National System of Management of Genetic Heritage and Associated Traditional Knowledge (SISGEN) under the number AD070A3. The red sacaca essential oil was obtained by hydrodistillation in a modified Clevenger apparatus (LCR Cientifica; Cosmopolis, Brasil) for 4 h, from plants collected between 08:00–09:00 a.m., as reported previously ( Azevedo et al, 2016 ; Silva et al, 2022 ).…”

Section: Methodsmentioning

confidence: 99%

Red sacaca essential oil-loaded nanostructured lipid carriers optimized by factorial design: cytotoxicity and cellular reactive oxygen species levels

Chura,

Memória,

Lopes

et al. 2023

Front. Pharmacol.

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Amazonian flora includes several species with the potential to develop pharmaceutical and biotechnological products. The essential oils from Amazonian species possess some biological properties, such as antioxidant, antibacterial, and cytotoxic activities. The essential oil of red sacaca (RSO), Croton cajucara Benth., contains metabolites characterized by antioxidant and anti-inflammatory activities. Nanostructured lipid carriers (NLC) are an advantageous alternative for the effective delivery of drugs because they can solubilize lipophilic actives and reduce their cytotoxicity. This study aimed to optimize the synthesis of RSO-loaded nanostructured lipid carriers (NLC-RSO) using a 23 factorial design and investigate their antioxidant and cytotoxic effects. The red sacaca essential oil (RSO) metabolite profile was characterized using gas chromatography coupled with a mass spectrometer (GC-MS), identifying 33 metabolites, with linalool and 7-hydroxy-calamenene as the major ones, as reported in the literature. The optimized NLC-RSO formulation had a particle size less than 100 nm and a polydispersity index lower than 0.25. After characterizing NLC-RSO using Fourier-transform infrared spectroscopy, powder X-ray diffraction, zeta potential, moisture content, and wettability, in vitro cytotoxicity were performed in A549 and BEAS-2B cell lines using the resazurin metabolism assay. The data indicated a lower IC50 for RSO than for NLC-RSOs in both cell lines. Furthermore, low cytotoxicity of blank nanoparticles (blank NP) and medium chain triglycerides-loaded nanostructured lipid carriers (NLC-MCT) towards both pulmonary cell lines was noted. At a concentration of 50–100 μg/mL, free RSO exhibited higher cytotoxicity than NLC-RSO, demonstrating the protective effect of this lipid carrier in reducing cytotoxicity during metabolite delivery. Similarly, free RSO showed higher 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging than NLC-RSO, also indicating this protective effect. The 2′,7′-dichlorofluorescein diacetate (DCFH-DA) intracellular reactive oxygen species (ROS) level assay did not show differences between the treatments at higher but non-cytotoxic dosages. Taken together, our results suggest that NLC-RSOs are potential RSO delivery systems for applications related to cancer treatment.

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Section: Methodsmentioning

confidence: 99%

Red sacaca essential oil-loaded nanostructured lipid carriers optimized by factorial design: cytotoxicity and cellular reactive oxygen species levels

Chura,

Memória,

Lopes

et al. 2023

Front. Pharmacol.

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“…Paralelamente ao uso inadequado de agentes antimicrobianos na prática médica humana, a aplicação desses medicamentos em animais de criação e na piscicultura está levantando preocupações substanciais, uma vez que é um dos principais impulsionadores da disseminação da resistência antimicrobiana (SANTOS et al, 2018). Dessa forma, a utilização indevida e indiscriminada de medicamentos tem contribuído para o surgimento de cepas resistentes, impactando tanto o ecossistema quanto a saúde e segurança da população em geral (SILVA et al, 2022).…”

Section: A Resistênciaunclassified

O Uso De Antibióticos Na Piscicultura

Israel,

Matos

2023

REASE

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O Brasil é um país com enorme potencial para a produção aquícola, destaca-se pela importância da piscicultura como uma das indústrias de produção de alimentos mais promissoras em âmbito nacional e global. Entretanto, o aumento da intensificação na produção de peixes, tem sido acompanhado pelo uso de antibióticos, tanto para profilaxia quando para a terapêutica de doenças bacterianas. No entanto, esse uso indiscriminado e excessivo de antibióticos na piscicultura traz consigo preocupações significativas, incluindo o desenvolvimento de resistência bacteriana e impactos negativos para o meio ambiente. Diante da problemática o presente trabalho tem por objetivo realizar uma revisão bibliográfica, abordando sobre as diversas facetas do uso de antibióticos na piscicultura brasileira, além disso, busca-se analisar as principais doenças bacterianas que afetam a piscicultura, identificar os agentes bacterianos envolvidos, compreender a resistência bacteriana que pode surgir devido ao uso de antibióticos e os impactos ambientais decorrentes dessa prática, como também abordar alternativas viáveis e sustentáveis ao uso indiscriminado de antibióticos. Desse modo, este estudo reforça a importância da gestão responsável do uso de antibióticos na piscicultura como um fator crítico para manter o equilíbrio entre a saúde dos peixes, a segurança alimentar e a preservação dos ecossistemas aquáticos. A compreensão abrangente dessas questões é fundamental para orientar políticas e práticas mais sustentáveis na indústria da piscicultura brasileira, garantindo sua prosperidade a longo prazo.

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Prospecting of essential oils in combination with florfenicol against motile Aeromonas isolated from tambaqui (Colossoma macropomum)

Cited by 2 publications

References 47 publications

Red sacaca essential oil-loaded nanostructured lipid carriers optimized by factorial design: cytotoxicity and cellular reactive oxygen species levels

Red sacaca essential oil-loaded nanostructured lipid carriers optimized by factorial design: cytotoxicity and cellular reactive oxygen species levels

O Uso De Antibióticos Na Piscicultura

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