Prospective analysis of circulating metabolites and breast cancer in EPIC

His, Mathilde; Viallon, Vivian; Dossus, Laure; Gicquiau, Audrey; Achaintre, David; Scalbert, Augustin; Ferrari, Pietro; Romieu, Isabelle; Onland‐Moret, N. Charlotte; Weiderpass, Elisabete; Dahm, Christina C.; Overvad, Kim; Olsen, Anja; Tjønneland, Anne; Fournier, Agnès; Rothwell, Joseph A.; Severi, Gianluca; Kühn, Tilman; Fortner, Renée T.; Boeing, Heiner; Trichopoulou, Antonia; Karakatsani, Anna; Martimianaki, Georgia; Masala, Giovanna; Sieri, Sabina; Tumino, Rosario; Víneis, Paolo; Panico, Salvatore; Gils, Carla H. van; Nøst, Therese Haugdahl; Sandanger, Torkjel M.; Skeie, Guri; Quiŕos, J. Ramón; Agudo, Antonio; Sánchez, María José; Amiano, Pilar; Huerta, José María; Ardanáz, Eva; Schmidt, Julie A.; Travis, Ruth C.; Riboli, Elio; Tsilidis, Konstantinos K.; Christakoudi, Sofia; Gunter, Marc J.; Rinaldi, Sabina

doi:10.1186/s12916-019-1408-4

Cited by 99 publications

(139 citation statements)

References 40 publications

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“…There are clear understanding on the heightened choline metabolism in cancer cells and tissues that support the progression, invasion and metastasis of cancer cells (9)(10)(11)(12)(13)(14)(15)(16)(17). In other way, cancer cells are known to divert the dietary source including choline to support the metabolic needs of breast carcinoma and other cancer cell types (11)(12)(13)(14)(15)(16).…”

Section: Level Of Choline and Phosphorylcholine In Nailsmentioning

confidence: 99%

“…Therefore, comprehensive and novel approaches are warranted to meet the requirements of early diagnosis, monitoring of drug response and new modalities of treatment options (3)(4)(5)(6). Therefore, new area of genomics, proteomics, transcriptomics, epigenomic and metabolomics is set to make advancement in exploring novel classes of biomarkers that can assist in the early detection and drug response monitoring avenues in a global context and Indian setting (3)(4)(5)(6)(7)(8)(9)(10).…”

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

“…The importance of metabolic heterogeneity is seen as one of key factors that contribute towards biomarkers development in breast cancer patients (5)(6)(7)(8)(9)(10)(11). Metabolic heterogeneity encompasses various metabolic adaptations including lipid remodeling by breast cancer cells to suffice the metabolic requirements in the tumor microenvironment (9)(10)(11)(12)(13)(14)(15)(16)(17). Therefore, appreciable attempts were made to determine lipid profiles mostly in blood, as it is the viable option to detect early and late stage physiological and pathological changes in breast cancer patients (10)(11)(12)(13)(14)(15)(16)(17).…”

Section: Introductionmentioning

confidence: 99%

“…Among key lipid metabolites, levels of choline derived lipids such as phosphatidylcholine (PC), phosphorylcholine and lyso-PC are suggested to decrease in breast cancer and other cancer types and these metabolites are seen as potential biomarkers (13)(14)(18)(19)(20)(21). In the current approach of lipid metabolomics, LC-HRMS, GC-MS and NMR spectroscopy were widely employed on whole metabolite extracts from blood and other biological fluid samples (10)(11)(12)(13)(14)(15)(16)(17)(18)(19)(20)(21).…”

Section: Introductionmentioning

confidence: 99%

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Novel approach reveals lipid metabolite reduction in nails of breast cancer patients as potential biomarker

Mitruka

Gore

Kumar

et al. 2020

Preprint

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BACKGROUND: Molecular adaptations in intracellular and extracellular microenvironment of breast cancer cells promote pro-tumor metabolic reprogramming. Hence, metabolic reprogramming is seen as a crucial factor in various tumor hallmarks including drug resistance, invasiveness and metastasis. Among well-known metabolic features of breast carcinoma including Warburg effects, altered amino acid metabolism, lipid remodeling is considered as key factors in achieving pro-tumor microenvironment. Therefore, a better understanding on molecular aspects of lipid remodeling is highly appreciated that may contribute towards future therapeutics and diagnostics purpose including the need of potential biomarkers. The identification and validation of lipid biomarkers are reported in the literature, but evidence on lipid metabolites as biomarkers in nails of breast cancer patients is completely unexplored. METHODS: This study reported a novel and specifically designed vertical tube gel electrophoresis (VTGE) system to assist in the purification of metabolites in the range of (~100-1000 Da) from nail samples. Fingernail clippings of breast cancer patients (N=10), and healthy subjects (N-12) were used for extraction and purification of metabolites. The VTGE system uses 15% polyacrylamide under non-denaturing and non-reducing conditions that makes eluted metabolites directly compatible with LC-HRMS and other analytical techniques. The characterization of lipid metabolites in nail lysates was done by positive ESI mode of Agilent LC-HRMS platform. RESULTS: Data suggest a novel observation that healthy and breast cancer patients show distinct accumulation of lipid metabolites specifically choline-based lipids. This is a first report that suggests that levels of choline, phosphorylcholine and lyso-PC are highly reduced and undetectable in nails of breast cancer patients over healthy subject. Furthermore, the potential use of reduced level of choline, phosphorylcholine and lyso-PC in nails of breast cancer patients is in line with current notion that these lipids are diverted to meet the pro-tumor activities in the tumor microenvironment. CONCLUSION: Data strongly provide a proof of concept for the potential use of lipid metabolites including choline, phosphorylcholine and lyso-PC as a set of biomarkers in nails of breast cancer patients. However, the authors propose that validity of these lipid biomarkers may be extended to large population size of breast cancer patients for future applications in early detection, grading, staging, predicting prognosis and therapeutic targeting of breast carcinoma.

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Section: Level Of Choline and Phosphorylcholine In Nailsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Novel approach reveals lipid metabolite reduction in nails of breast cancer patients as potential biomarker

Mitruka

Gore

Kumar

et al. 2020

Preprint

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Decoding chronic rhinosinusitis: A metabolomics‐based approach

Gong,

Fu,

Zhou

et al. 2024

Int Forum Allergy Rhinol

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BackgroundChronic rhinosinusitis (CRS) is a common and intractable disease in otorhinolaryngology, laying a heavy burden on healthcare systems. The worldwide researchers are making efforts to find solutions to this disease. Metabolomics has recently gained more and more traction, and might become a promising tool to unravel the complexity of CRS. This paper provides an overview of current studies on the metabolomics of various CRS subtypes.MethodsWe conducted a comprehensive literature search in PubMed, Web of Science, EMBASE, Google Scholar, and Cochrane Library, up to May 25, 2023. Search strategies incorporated key terms such as “chronic rhinosinusitis” and “metabolomics” with relevant synonyms and MeSH terms. Titles and abstracts of 86 screened articles were assessed for relevance to CRS and metabolomics. Methodological robustness, data reliability, and relevance were considered for shortlisted articles.ResultsAfter the refined process, a total of 26 articles were included in this study and sorted out by research themes, methodology and pivotal discoveries. These included studies identified the metabolic pathways and markers related to the pathophysiology in each subtype of CRS.ConclusionsMetabolomics helps to shed light on the complexity of CRS. The mentioned findings highlight the importance of specific metabolic pathways and markers in understanding the pathophysiology of CRS. Despite that, challenges and future directions in metabolomics research for CRS would be worth being further explored.

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Metabolic perturbations prior to hepatocellular carcinoma diagnosis: Findings from a prospective observational cohort study

Stępień

Keski‐Rahkonen

Kiss

et al. 2020

Intl Journal of Cancer

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Hepatocellular carcinoma (HCC) development entails changes in liver metabolism. Current knowledge on metabolic perturbations in HCC is derived mostly from case‐control designs, with sparse information from prospective cohorts. Our objective was to apply comprehensive metabolite profiling to detect metabolites whose serum concentrations are associated with HCC development, using biological samples from within the prospective European Prospective Investigation into Cancer and Nutrition (EPIC) cohort (>520 000 participants), where we identified 129 HCC cases matched 1:1 to controls. We conducted high‐resolution untargeted liquid chromatography‐mass spectrometry‐based metabolomics on serum samples collected at recruitment prior to cancer diagnosis. Multivariable conditional logistic regression was applied controlling for dietary habits, alcohol consumption, smoking, body size, hepatitis infection and liver dysfunction. Corrections for multiple comparisons were applied. Of 9206 molecular features detected, 220 discriminated HCC cases from controls. Detailed feature annotation revealed 92 metabolites associated with HCC risk, of which 14 were unambiguously identified using pure reference standards. Positive HCC‐risk associations were observed for N1‐acetylspermidine, isatin, p‐hydroxyphenyllactic acid, tyrosine, sphingosine, l,l‐cyclo(leucylprolyl), glycochenodeoxycholic acid, glycocholic acid and 7‐methylguanine. Inverse risk associations were observed for retinol, dehydroepiandrosterone sulfate, glycerophosphocholine, γ‐carboxyethyl hydroxychroman and creatine. Discernible differences for these metabolites were observed between cases and controls up to 10 years prior to diagnosis. Our observations highlight the diversity of metabolic perturbations involved in HCC development and replicate previous observations (metabolism of bile acids, amino acids and phospholipids) made in Asian and Scandinavian populations. These findings emphasize the role of metabolic pathways associated with steroid metabolism and immunity and specific dietary and environmental exposures in HCC development.

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Prospective analysis of circulating metabolites and breast cancer in EPIC

Cited by 99 publications

References 40 publications

Novel approach reveals lipid metabolite reduction in nails of breast cancer patients as potential biomarker

Novel approach reveals lipid metabolite reduction in nails of breast cancer patients as potential biomarker

Decoding chronic rhinosinusitis: A metabolomics‐based approach

Metabolic perturbations prior to hepatocellular carcinoma diagnosis: Findings from a prospective observational cohort study

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