Prospective Comparison of Blood Culture Bottles and Conventional Swabs for Microbial Identification of Suspected Periprosthetic Joint Infection

Differences in Diagnostic Properties Between Standard and Enrichment Culture Techniques Used in Periprosthetic Joint Infections

“…In the current study, the observation that several inflammatory markers, all of which are important parameters for diagnosing PJI [21], were significantly lower in the enrichment culture group than in the standard culture group has important clinical implications. In agreement with our results, Geller et al [18] also reported that the synovial fluid WBC, synovial WBC neutrophil percentage, and CRP levels of samples tested positive by a blood culture bottle method only were significantly lower than those in the culture swabpositive group. This demonstrates that cases with a lower level of systemic inflammation that are culture-negative by standard methods are detectable using enriched culture media, suggesting that this is an important approach for the diagnosis of cases that prove inconclusive by standard methods [2].…”

“…Blackmur et al [20] reported that enrichment culture showed a sensitivity of 95% and specificity of 91% in patients suspected of arthritis or PJI, and it was more effective than standard culture practice. In addition, Geller et al [18] reported that the blood culture bottle system was more effective in identifying infecting organisms in PJI than traditional culture by swab samples. Minassian et al [17] also reported the utility of a blood culture system for the diagnosis of PJI in terms of higher sensitivity, specificity, and speed.…”

Escherichia vulneris como agente etiológico de artritis séptica en una niña

“…Cabe destacar, que dado el carácter fastidioso de algunos de los agentes etiológicos de artritis séptica en niños y a que las muestras de líquido articular pueden ser de escaso volumen, se requieren métodos altamente sensibles para la detección del microorganismo causal. En este contexto, el uso de frascos de hemocultivos provee de un medio enriquecido para el desarrollo bacteriano 11 . Varios autores han demostrado que los viales de hemocultivo presentan un rendimiento superior a otros métodos convencionales.…”

“…Varios autores han demostrado que los viales de hemocultivo presentan un rendimiento superior a otros métodos convencionales. Además, la mayoría de los patógenos causales de artritis séptica se desarrollan dentro del período de incubación de un hemocultivo automatizado (cinco días o menos) 11,12 . Por esto, el uso de sistemas automatizados de hemocultivos para el cultivo de líquidos normalmente estériles es cada vez más aceptado 12 .…”

New Technologies for Diagnosis of Periprosthetic Hip Infection

“…64 This has been partially mitigated by the inoculation of fluid into blood culture bottles. 67,68 There are several other topics that have been the subject of much debate surrounding culture, with the optimal number of tissue samples obtained chief among those. There is an informal consensus that one sample is far from ideal, as it lacks the sensitivity to detect PJI, while a single positive culture, particularly with a known contaminant, can cause significant diagnostic uncertainty.…”