Prospective evaluation of rapid antimicrobial susceptibility testing by disk diffusion on Mueller-Hinton rapid-SIR directly on blood cultures

Périllaud, Claire; Pilmis, Benoît; Diep, Julien; Ponfilly, Gauthier Péan de; Vidal, Bárbara; Couzigou, C.; Mizrahi, Assaf; Lourtet-Hascoët, Julie; Monnier, Alban Le; Van, Jean-Claude Nguyen

doi:10.1016/j.diagmicrobio.2018.07.016

Cited by 26 publications

(19 citation statements)

References 24 publications

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“…However, direct antimicrobial susceptibility testing with reading of inhibition zones after 4–8 h of incubation has been used since the 1980s. 26 , 27 Also, attempts have been made to standardize an inoculum from 4–6 h subcultures or from positive BC broth 28–30 but without shortened incubation time, early reading and adapted breakpoints.…”

Section: Discussionmentioning

confidence: 99%

EUCAST rapid antimicrobial susceptibility testing (RAST) in blood cultures: validation in 55 European laboratories

Åkerlund

Jonasson

Matuschek

et al. 2020

Journal of Antimicrobial Chemotherapy

109

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Objectives When bloodstream infections are caused by resistant bacteria, rapid antimicrobial susceptibility testing (RAST) is important for adjustment of therapy. The EUCAST RAST method, directly from positive blood cultures, was validated in a multi-laboratory study in Europe. Methods RAST was performed in 40 laboratories in northern Europe (NE) and 15 in southern Europe (SE) from clinical blood cultures positive for Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, Staphylococcus aureus or Streptococcus pneumoniae. Categorical results at 4, 6 and 8 h of incubation were compared with results for EUCAST standard 16–20 h disc diffusion. The method, preliminary breakpoints and the performance of the laboratories were evaluated. Results The total number of isolates was 833/318 in NE/SE. The number of zone diameters that could be read (88%, 96% and 99%) and interpreted (70%, 81% and 85%) increased with incubation time (4, 6 and 8 h). The categorical agreement was acceptable, with total error rates in NE/SE of 2.4%/4.9% at 4 h, 1.1%/3.5% at 6 h and 1.1%/3.3% at 8 h. False susceptibility at 4, 6 and 8 h of incubation was below 0.3% and 1.1% in NE and SE, respectively, and the corresponding percentages for false resistance were below 1.9% and 2.8%. After fine-tuning breakpoints, more zones could be interpreted (73%, 89% and 93%), with only marginally affected error rates. Conclusions The EUCAST RAST method can be implemented in routine laboratories without major investments. It provides reliable antimicrobial susceptibility testing results for relevant bloodstream infection pathogens after 4–6 h of incubation.

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Section: Discussionmentioning

confidence: 99%

EUCAST rapid antimicrobial susceptibility testing (RAST) in blood cultures: validation in 55 European laboratories

Åkerlund

Jonasson

Matuschek

et al. 2020

Journal of Antimicrobial Chemotherapy

109

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“…Antimicrobial assay was performed in triplicate 31. , 40. , and the data are expressed as means ± SD ( Table 2 ).…”

Section: Methodsmentioning

confidence: 99%

“…Streptomycin and tinidazole (Sigma–Aldrich, USA) were co-assayed as positive controls for the pathogenic bacteria and fungi, respectively. Antimicrobial assay was performed in triplicate31., 40., and the data are expressed as means ± SD (Table 2).…”

Section: Methodsmentioning

confidence: 99%

Cytotoxic and antibacterial polyketide-indole hybrids synthesized from indole-3-carbinol by Daldinia eschscholzii

Lin

Jiang

Mei

et al. 2019

Acta Pharmaceutica Sinica B

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Two skeletally undescribed polyketide-indole hybrids (PIHs), named indolchromins A and B, were generated from indole-3-carbinol (I3C) in the fungal culture ( Daldinia eschscholzii ). The indolchromin structures were elucidated mainly by their 1D and 2D NMR spectra with the former confirmed by the single-crystal X-ray crystallographic analysis. Each indolchromin alkaloid was chirally separated into four isomers, whose absolute configurations were assigned by comparing the recorded circular dichroism (CD) spectra with the electronic CD (ECD) curves computed for all optional stereoisomers. Furthermore, the indolchromin construction pathways in fungal culture were clarified through enzyme inhibition, precursor feeding experiment, and energy calculation. The cascade reactions, including decarboxylative Claisen condensation catalyzed by 8-amino-7-oxononanoate synthase (AONS), C( sp 3 )-H activation, double bond migration, and Michael addition, all undergone compatibly during the fungal cultivation. In an MIC range of 1.3–8.6 μmol/L, (2 S ,4 R )- and (2 R ,4 S )-indolchromin A and (2 R ,4 S )-indolchromin B are inhibitory against Clostridium perfringens , Clostridium difficile , Veillonella sp., Bacteroides fragilis , and Streptococcus pyogenes . (2 R ,4 S )-Indolchromin A and (2 S ,4 S )-indolchromin B were cytotoxic against the human breast cancer cell line MDA-MB-231 with IC 50 values of 27.9 and 131.2 nmol/L, respectively, with the former additionally active against another human breast cancer cell line MCF-7 (IC 50 94.4 nmol/L).

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“…The Accelerate Pheno system has been previously cited and allows phenotypic antimicrobial susceptibility testing by morpho-kinetic cellular analysis with time to result of approximately 7 h. Other rapid AST phenotypic methods include the automated broth microdilution method by Phoenix and Vitek, disk diffusion methods, and gradient strips using Etest (bioMérieux) or MIC Strip (Liofilchem) [ 61 , 62 , 63 ]. Rapid AST can be also performed directly on blood culture samples tested on Mueller–Hinton Rapid agar (MHR-SIR) with a time delay of 6–8 h associated with a significant time saving (17 h), according to the appropriateness of the antibiotic prescription [ 64 , 65 ]. AST by Alfred AST system performed directly from positive blood bottles has also been recently reported.…”

Section: Other Rapid Antimicrobial Susceptibility Testing (Ast) Phmentioning

confidence: 99%

Rapid Detection of Methicillin-Resistant Staphylococcus aureus Directly from Blood for the Diagnosis of Bloodstream Infections: A Mini-Review

et al. 2020

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Staphylococcus aureus represents a major human pathogen able to cause a number of infections, especially bloodstream infections (BSI). Clinical use of methicillin has led to the emergence of methicillin-resistant S. aureus (MRSA) and MRSA-BSI have been reported to be associated with high morbidity and mortality. Clinical diagnosis of BSI is based on the results from blood culture that, although considered the gold standard method, is time-consuming. For this reason, rapid diagnostic tests to identify the presence of methicillin-susceptible S. aureus (MSSA) and MRSA isolates directly in blood cultures are being used with increasing frequency to rapidly commence targeted antimicrobial therapy, also in the light of antimicrobial stewardship efforts. Here, we review and report the most common rapid non-molecular and molecular methods currently available to detect the presence of MRSA directly from blood.

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Prospective evaluation of rapid antimicrobial susceptibility testing by disk diffusion on Mueller-Hinton rapid-SIR directly on blood cultures

Cited by 26 publications

References 24 publications

EUCAST rapid antimicrobial susceptibility testing (RAST) in blood cultures: validation in 55 European laboratories

EUCAST rapid antimicrobial susceptibility testing (RAST) in blood cultures: validation in 55 European laboratories

Cytotoxic and antibacterial polyketide-indole hybrids synthesized from indole-3-carbinol by Daldinia eschscholzii

Rapid Detection of Methicillin-Resistant Staphylococcus aureus Directly from Blood for the Diagnosis of Bloodstream Infections: A Mini-Review

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