Prospective Evaluation of the Defecatory Functional Results in Patients Following Aorto-Aortic Reconstruction Surgery for an Abdominal Aortic Aneurysm

Dobrowolski, Sebastian; Wojciechowski, Jacek; Dobosz, Marek; Hać, Stanisław; Śledziński, Zbigniew

doi:10.1007/s00595-007-3511-y

Cited by 4 publications

(1 citation statement)

References 24 publications

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“…Here it might be hypothesized, that gelatin (as derivative from collagen) would be recognized as part of the subendothelial matrix, inducing intrinsic coagulation pathways [8]. While Jensen et al [17] described a comparable thrombogenicity of gelatin and collagen, some recent studies revealed that gelatin is a low thrombogenic biomaterial, which is frequently used as a hemocompatible coating for scientific or commercial cardiovascular applications [9,42]. It can be assumed that the material processing plays a role for the accessibility of thrombogenic recognition sides on the gelatin surface.…”

Section: Secretion Of Vasoactive Mediators and Cytokinesmentioning

confidence: 99%

Monolayer formation and shear- resistance of human vein endothelial cells on gelatin-based hydrogels with tailorable elasticity and degradability

Schulz

Vukićević

Krüger‐Genge

et al. 2017

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The formation of a functionally-confluent and shear-resistant endothelial cell (EC) monolayer on cardiovascular implants is a promising strategy to prevent thrombogenic processes after implantation. On the basis of existing studies with arterial endothelial cells adhering after two hours on gelatin-based hydrogels in marked higher numbers compared to tissue culture plates, we hypothesized that also venous endothelial cells (HUVEC) should be able to adhere and form an endothelial monolayer on these hydrogels after days. Furthermore, variation of the hydrogel composition, which slightly influences the materials elasticity and even more the degradation behaviour, should have no considerable effect on HUVEC. Therefore, the monolayer formation and shear resistance of HUVEC were explored on two gelatin-based hydrogels differing in their elasticity (Young's moduli between 35 and 55 kPa) in comparison to a positive control (HUVEC on glass cover slips) and a negative control (HUVEC on glass cover slips activated with interleukin-1β) after 9 days of culturing. HUVEC density after 9 days of culturing under static conditions was lower on the hydrogels compared to both controls (p < 0.05 each). On G10_LNCO8 slightly more EC adhered than on G10_LNCO5. Staining of the actin cytoskeleton and VE-cadherin revealed a pronounced cell-substrate interaction while the cell-cell interaction was comparable to the controls (HUVEC on glass). The secretion of vasoactive and inflammatory mediators did not differ between the hydrogels and the controls. Adherent HUVEC seeded on the hydrogels were able to resist physiological shear forces and the release of cyto- and chemokines in response to the shear forces did not differ from controls (HUVEC on glass). Therefore, both gelatin-based hydrogels are a suitable substrate for EC and a promising candidate for cardiovascular applications.

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Section: Secretion Of Vasoactive Mediators and Cytokinesmentioning

confidence: 99%

Monolayer formation and shear- resistance of human vein endothelial cells on gelatin-based hydrogels with tailorable elasticity and degradability

Schulz

Vukićević

Krüger‐Genge

et al. 2017

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New insights in the neuroanatomy of the human adult superior hypogastric plexus and hypogastric nerves

Kraima

Schaik

Susan

et al. 2015

Autonomic Neuroscience

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Pilot Study on Feasibility and Outcome of a Nerve-Preserving Aortoiliac Exposure Technique

Schaik

Witt

Albers

et al. 2024

Annals of Vascular Surgery

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Prospective Evaluation of the Defecatory Functional Results in Patients Following Aorto-Aortic Reconstruction Surgery for an Abdominal Aortic Aneurysm

Abstract: An injury to the intermesenteric, inferior mesenteric, and superior hypogastric plexuses does not significantly influence the defecatory functions in patients following abdominal reconstruction surgery for an abdominal aortic aneurysm.

Cited by 4 publications

References 24 publications

Monolayer formation and shear- resistance of human vein endothelial cells on gelatin-based hydrogels with tailorable elasticity and degradability

Monolayer formation and shear- resistance of human vein endothelial cells on gelatin-based hydrogels with tailorable elasticity and degradability

New insights in the neuroanatomy of the human adult superior hypogastric plexus and hypogastric nerves

Pilot Study on Feasibility and Outcome of a Nerve-Preserving Aortoiliac Exposure Technique

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