Prospective Evaluation of Whole Genome MicroRNA Expression Profiling in Childhood Acute Lymphoblastic Leukemia

Duyu, Muhterem; Durmaz, Burak; Gündüz, Cumhur; Vergin, Canan; Karapınar, Deniz Yılmaz; Aksoylar, Serap; Kavaklı, Kaan; Çetingül, Nazan; İrken, Gülersu; Yaman, Yontem; Özkınay, Ferda; Çoğulu, Özgür

doi:10.1155/2014/967585

Cited by 52 publications

(58 citation statements)

References 36 publications

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“…If miR-146a is overexpressed in hematopoietic stem cells and bone marrow transplantation is conducted, instantaneous marrow expansion will occur, erythropoiesis will be reduced and lymphocytopoiesis will be suppressed (8). Increased miR-146a expression is deleterious for marrow reconstruction in mice and reduces the survival rate of hematopoietic stem cells (9).…”

Section: Discussionmentioning

confidence: 99%

The effect of miR-146a on STAT1 expression and apoptosis in acute lymphoblastic leukemia Jurkat cells

Wang¹,

Guo²,

Suo³

et al. 2016

Oncology Letters

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The effect of miR-146a-dependent regulation of STAT1 on apoptosis in acute lymphoblastic leukemia (ALL) Jurkat cells was investigated. The miR-146a mimic and miR-146a inhibitor vectors were constructed in vitro, and experimental grouping was as follows: Control group (untreated Jurkat cells), empty vector group (Jurkat cells transfected with empty vector), agonist group (Jurkat cells transfected with miR-146a mimic) and the inhibitor group (Jurkat cells transfected with miR-146a inhibitor). Western blot analysis was used to observe the expression, respectively, of STAT1, p-STAT1 and Bcl-xL, and flow cytometry was used to test apoptosis in Jurkat cells. STAT1 and p-STAT1 expression in the agonist group was higher than that in the control and empty vector groups, but lower in the inhibitor group, and differences were statistically significant (P<0.05). The rate of apoptosis in the agonist group was significantly higher than that of the control group and blank vector group, and it was significantly lower in the inhibitor group (P<0.05). As a tumor suppressor, miR-146a can regulate expression of apoptosis-promoting factor STAT1, and anti-apoptosis factor Bcl-xL, and is able to promote apoptosis of ALL Jurkat cells.

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Section: Discussionmentioning

confidence: 99%

The effect of miR-146a on STAT1 expression and apoptosis in acute lymphoblastic leukemia Jurkat cells

Wang¹,

Guo²,

Suo³

et al. 2016

Oncology Letters

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“…Later on, several studies reported dysregulated miRNA expression in pediatric ALL samples compared to normal cells. For example, a number of publications described increased expression of miR-21, miR-34, miR-128, miR-142, miR-146a, miR-181b, miR-195, and miR-708 [ 19 , 20 , 21 , 22 , 23 ], and decreased levels of miR-18, miR-181a, miR-99a, miR-100, miR-145, let-7, and miR-196b in ALL cells [ 19 , 21 , 24 , 25 ].…”

Section: Leukemiasmentioning

confidence: 99%

“…The expression of some miRNAs can be used to monitor disease progression, such as with miR-128, miR-146a, miR-155, miR-181a, and miR-195 [ 21 ]. In addition, miR-210 has been proposed as a good prognostic factor and a useful predictor of drug sensitivity [ 53 ].…”

Section: Leukemiasmentioning

confidence: 99%

MiRNA Dysregulation in Childhood Hematological Cancer

Oliveira

Roberto

Baroni

et al. 2018

IJMS

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For decades, cancer biology focused largely on the protein-encoding genes that have clear roles in tumor development or progression: cell-cycle control, apoptotic evasion, genome instability, drug resistance, or signaling pathways that stimulate growth, angiogenesis, or metastasis. MicroRNAs (miRNAs), however, represent one of the more abundant classes of cell modulators in multicellular organisms and largely contribute to regulating gene expression. Many of the ~2500 miRNAs discovered to date in humans regulate vital biological processes, and their aberrant expression results in pathological and malignant outcomes. In this review, we highlight what has been learned about the roles of miRNAs in some of the most common human pediatric leukemias and lymphomas, along with their value as diagnostic/prognostic factors.

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“… 66 Other groups also have performed analogous studies to discover miRNAs expression signatures of ALL. 67 – 70 …”

Section: The Function Of Mirnas In Allmentioning

confidence: 99%

The functional role of microRNA in acute lymphoblastic leukemia: relevance for diagnosis, differential diagnosis, prognosis, and therapy

Luan

Yang

Chen

2015

OTT

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MicroRNAs (miRNAs), a new class of noncoding RNAs, which can hybridize to target messenger RNAs and regulate their expression posttranscriptionally, express differentially in distinct stages of lymphopoiesis and influence the direction of lymphoid precursor maturation. Hence, there is aberrant expression of miRNAs involved in malignant lymphopoiesis, and these aberrations can be used as signatures of acute lymphoblastic leukemia (ALL) with different subtypes. In addition, changes in the expression of several miRNAs may have functional relevance with leukemogenesis or drug resistance. As a result, the reversal of the expression of these miRNAs may alleviate the disease to some extent and improve clinical outcomes. However, among the studies of miRNAs, there are still some problems that need to be solved to understand the function of miRNAs in ALL more thoroughly.

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Prospective Evaluation of Whole Genome MicroRNA Expression Profiling in Childhood Acute Lymphoblastic Leukemia

Cited by 52 publications

References 36 publications

The effect of miR-146a on STAT1 expression and apoptosis in acute lymphoblastic leukemia Jurkat cells

The effect of miR-146a on STAT1 expression and apoptosis in acute lymphoblastic leukemia Jurkat cells

MiRNA Dysregulation in Childhood Hematological Cancer

The functional role of microRNA in acute lymphoblastic leukemia: relevance for diagnosis, differential diagnosis, prognosis, and therapy

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