Prospective randomized controlled study comparing cell block method and conventional smear method for bile cytology

Noda, Yutaka; Fujita, Naotaka; Kobayashi, Go; Ito, Kei; Horaguchi, Jun; Hashimoto, Shinichi; Koshita, Shinsuke; Ishii, Shotaro; Kanno, Yoshihide; Ogawa, Takahisa; Masu, Kaori; Tsuchiya, Takashi; Oikawa, Masaya; Honda, Hiroshi; Sawai, Takashi; Uzuki, Miwa; Fujishima, Fumiyoshi

doi:10.1111/j.1443-1661.2012.01404.x

Cited by 14 publications

(15 citation statements)

References 42 publications

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“…The mutated tumors that have been identified were in intrahepatic cholangiocarcinoma . Further studies are needed to evaluate the possibility of using cell blocks of brushing and bile samples for these molecular analyses …”

Section: Discussionmentioning

confidence: 99%

Monocentric study of bile aspiration associated with biliary brushing performed during endoscopic retrograde cholangiopancreatography in 239 patients with symptomatic biliary stricture

Fior‐Gozlan

Giovannini

Rabeyrin

et al. 2015

Cancer Cytopathology

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Section: Discussionmentioning

confidence: 99%

Monocentric study of bile aspiration associated with biliary brushing performed during endoscopic retrograde cholangiopancreatography in 239 patients with symptomatic biliary stricture

Fior‐Gozlan

Giovannini

Rabeyrin

et al. 2015

Cancer Cytopathology

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“…The cell block method can also generate multiple sections for staining and immunohistochemistry[ 14 ]. The efficacy of cell block method has been reported in the bile duct cytology and endoscopic ultrasound-guided fine needle aspiration of pancreas and gastrointestinal solid neoplastic lesions[ 7 - 9 ].…”

Section: Discussionmentioning

confidence: 99%

Case of pancreatic metastasis from colon cancer in which cell block using the Trefle^® endoscopic scraper enables differential diagnosis from pancreatic cancer

Naitoh

Kato

Hayashi

et al. 2018

WJGO

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Endoscopic transpapillary brush cytology and forceps biopsy during endoscopic retrograde cholangiopancreatology are generally used to obtain pathological evidence of biliary strictures. Recently, the new endoscopic scraper Trefle® has been reported and demonstrated high cancer detectability in malignant biliary strictures. This device is used to scrape the stricture over the guidewire, and, in the original method, the tissue and/or cell samples obtained are subjected to histological and/or cytological analysis separately. However, discrimination of chunks of tissue is hampered by the opacity of the surrounding fluid. We have developed a cell block technique for the Trefle® device without dividing obtained specimens into tissue and cellular components, which is the simplest method and enables immunohistochemical analysis. We present a case of obstructive jaundice diagnosed immunohistochemically as pancreatic metastasis from colon cancer using cell block sections obtained with the Trefle® device, which procedure is as easy as conventional brush cytology.

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“…To improve the sensitivity and specificity, many investigators employ various sampling techniques, new devices, the cell block method and molecular methods, such as k-ras point mutation analysis or FISH [7,8,9,10,11,12,13,14,15,16,17,18,19,20]. In some hospitals, to facilitate smear sampling, rapid on-site evaluation is used to prevent air-drying and insufficient sampling [20].…”

Section: Discussionmentioning

confidence: 99%

Brushing Cytology of the Biliary Tract: Bile Juice from the ERCP Sheath Tube Provides Cell-Rich Smear Samples

Wakasa

Inayama²,

Honda³

et al. 2014

Acta Cytologica

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Objectives: To improve the diagnostic accuracy of bile smear cytology, we assessed two cell-yielding procedures. Study Design: One hundred and forty-one patients with biliary stricture underwent endoscopic retrograde cholangiopancreatography (ERCP) and conventional brush sampling. The cytologist cut the brush head off the support wire, centrifuged it directly in tissue culture medium for 1 min at 3,000 rpm, centrifuged the medium again and then smeared the cell pellet onto slides. The remaining sheath tube was then cut into 12-cm segments, which were centrifuged in a centrifuge tube for 1 min at 3,000 rpm, collected and submitted for cytospin preparation. Results: The final histopathological diagnoses based on surgery, biopsy or clinical progression were evaluated for sensitivity, specificity and accuracy. Using conventional smears alone, the sensitivity, specificity and accuracy in patients with biliary stricture were 66.1, 80.7 and 68.8%, respectively. For conventional smears, brush washing and sheath tube contents together, the sensitivity improved to 73.9%, specificity to 100% and accuracy to 78.7%. In the patients with bile duct carcinoma, the sensitivity, specificity and accuracy were 87.3, 100 and 90.7%, respectively. Conclusion: Superior diagnostic accuracy was achieved when conventional smear procedures were combined with the two new procedures.

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Prospective randomized controlled study comparing cell block method and conventional smear method for bile cytology

Abstract: The CB method for bile cytology showed a higher diagnostic yield than smear cytology. Its diagnostic sensitivity was satisfactory in cases of bile duct cancer.

Cited by 14 publications

References 42 publications

Monocentric study of bile aspiration associated with biliary brushing performed during endoscopic retrograde cholangiopancreatography in 239 patients with symptomatic biliary stricture

Monocentric study of bile aspiration associated with biliary brushing performed during endoscopic retrograde cholangiopancreatography in 239 patients with symptomatic biliary stricture

Case of pancreatic metastasis from colon cancer in which cell block using the Trefle^® endoscopic scraper enables differential diagnosis from pancreatic cancer

Brushing Cytology of the Biliary Tract: Bile Juice from the ERCP Sheath Tube Provides Cell-Rich Smear Samples

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Prospective randomized controlled study comparing cell block method and conventional smear method for bile cytology

Abstract: The CB method for bile cytology showed a higher diagnostic yield than smear cytology. Its diagnostic sensitivity was satisfactory in cases of bile duct cancer.

Cited by 14 publications

References 42 publications

Monocentric study of bile aspiration associated with biliary brushing performed during endoscopic retrograde cholangiopancreatography in 239 patients with symptomatic biliary stricture

Monocentric study of bile aspiration associated with biliary brushing performed during endoscopic retrograde cholangiopancreatography in 239 patients with symptomatic biliary stricture

Case of pancreatic metastasis from colon cancer in which cell block using the Trefle® endoscopic scraper enables differential diagnosis from pancreatic cancer

Brushing Cytology of the Biliary Tract: Bile Juice from the ERCP Sheath Tube Provides Cell-Rich Smear Samples

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Case of pancreatic metastasis from colon cancer in which cell block using the Trefle^® endoscopic scraper enables differential diagnosis from pancreatic cancer