Prospective Study ofCRMP4Promoter Methylation in Prostate Biopsies as a Predictor For Lymph Node Metastases

Abstract

CRMP4 promoter methylation in diagnostic biopsies could be a robust biomarker for LNM in PCa.

SEC-induced activation of ANXA7 GTPase suppresses prostate cancer metastasis

“…7, A and B). The lymph node is a frequent metastatic site for prostate cancer[38]. Then, aortic lymph nodes were isolated and followed by bioluminescence in vitro imaging.…”

Calpain‐2 triggers prostate cancer metastasis via enhancing CRMP4 promoter methylation through NF‐κB/DNMT1 signaling pathway

“…Hypermethylation of the 5′ CpG island of CRMP4 promoter is responsible for CRMP4 repression in metastatic PCa, which was validated by locus‐specific manipulation of CpG methylation . Recently, we performed a multicenter prospective study and discovered that the levels of CRMP4 promoter methylation in biopsy specimens using pyrosequencing could accurately predict lymph nodes metastasis and poor prognosis after radical prostatectomy in cases with clinically localized PCa, which provided clinical evidence for the critical role of CRMP4 in PCa metastasis . Therefore, it reinforces our understanding of the mechanisms on how the methylation on CRMP4 promoter occurs.…”

“…Moreover, our results confirmed that S276 phosphorylation is essential for NF‐κB RelA/p65 in direct binding to CRMP4 promoter and interaction with DNMT1. To validate the clinical value of DNA methylation of CRMP4, we accomplished a prospective multicenter study and reported that determination on the level of CRMP4 promoter methylation with a cutoff value of 15% in prostate biopsies could accurately predict lymph node metastases . In addition, patients with higher level of promoter methylation tend to have worse prognosis.…”

CRMP4a suppresses cell motility by sequestering RhoA activity in prostate cancer cells

“…CRMP4a is the shorter splicing variant compared with CRMP4b and has been identified as a tumor metastasis suppressor in prostate cancers 8 . Proteomic and genomic analyses revealed that CRMP4a expression was significantly reduced in metastatic tumor tissues mainly due to promoter methylation [22][23][24] . CRMP4 expression could be enhanced at the transcriptional level by Figure 6.…”