Prospective study of infectious and noninfectious diseases in oysters and fishes in three Gulf of Mexico estuaries

Ja, Couch

doi:10.3354/dao001059

Cited by 54 publications

(42 citation statements)

References 29 publications

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“…Papillomas have been observed in the skin offish from industrially polluted water (Smith and Zaidlik 1987). Their occurrence indicates that cadmium is carcinogenic at high concentrations (Couch 1985).…”

Section: Capillariesmentioning

confidence: 99%

Effects of water-borne cadmium on the skin of the common carp (Cyprinus carpio)

Iger

Lock

Meij

et al. 1994

Arch. Environ. Contam. Toxicol.

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Section: Capillariesmentioning

confidence: 99%

Effects of water-borne cadmium on the skin of the common carp (Cyprinus carpio)

Iger

Lock

Meij

et al. 1994

Arch. Environ. Contam. Toxicol.

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“…Pathology is now a standard part of environmental monitoring programmes on pollution effects (Yevich & Barszcz 1976, Balouet & Poder 1981, Couch 1985. Based on an easily reproducible technique, histopathological studies yield basic information on tissue disorders related to the general state of organisms, and assess the host's susceptibility to infectious diseases and parasitic infestation.…”

Section: Methodsmentioning

confidence: 99%

Histopathological changes related to chemical contamination in Mytilus edulis from field and experimental conditions

Auffret¹

1988

Mar. Ecol. Prog. Ser.

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This comparative histopathological study, carried out at the GEEP Workshop, assesses tissue changes in mussels Mytilus edulis from a contaminated Norwegian fjord and from mesocosm basins where a contaminant gradient had been simulated. Parasitism by larval trematodes was observed in both circumstances, but was not related to contaminant levels. Mussels from the field exhibited granulocytomas in their interstitial tissues, with a greater incidence at polluted sites than at a reference site, suggesting that granulocytomas could be a consequence of chronic pollutant exposure. This pathological condition was not observed in experimentally treated mussels, which nevertheless had severe tissue alterations, especially in digestive tubules and gills, under exposure to high levels of a diesel oil and copper mixture. It is concluded that this type of histopathological analysis can provide useful information on the health of mussels, and that this information can be used successfully in the comparison of field samples.

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“…The Ulrich et al (2007) study did not include histological confirmation of the PCR results, whereas the negative results that we found in our PCR assay were consistent with those from histological examination of the same oysters, as well as previous histological surveys of Crassostrea virginica in the Gulf of Mexico, including the annual Mussel Watch analyses that began in 1995. Since at least 1978, several thousand oysters have been examined histologically without finding any recognizable haplosporidian (Couch 1985, Gauthier et al 1990, Fisher et al 1996, Kim et al 1998, Fisher et al 2000, Winstead et al 2004, Kim & Powell 2006. In addition, at least one other study has examined Gulf of Mexico oysters using both PCR and histology.…”

Section: Discussionmentioning

confidence: 99%

“…Dis Aquat Org 93: [251][252][253][254][255][256] 2011 C. virginica from Nova Scotia, Canada, to Biscayne Bay, Florida, USA (Haskin & Andrews 1988, Barber et al 1997, Stephenson et al 2003, but none of the several histological studies of oysters in the Gulf of Mexico have reported H. nelsoni or any other haplosporidian (Couch 1985, Gauthier et al 1990, Fisher et al 1996, Kim et al 1998, Winstead et al 2004, Kim & Powell 2006. The most comprehensive of these is the annual status and trends (S&T) …”

Section: Resale or Republication Not Permitted Without Written Consenmentioning

confidence: 99%

Widespread survey finds no evidence of Haplosporidium nelsoni (MSX) in Gulf of Mexico oysters

Ford

Paterno²,

Scarpa³

et al. 2011

Dis. Aquat. Org.

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The advent of molecular detection assays has provided a set of very sensitive tools for the detection of pathogens in marine organisms, but it has also raised problems of how to interpret positive signals that are not accompanied by visual confirmation. PCR-positive results have recently been reported for Haplosporidium nelsoni (MSX), a pathogen of the oyster Crassostrea virginica in 31 of 40 oysters from 6 sites in the Gulf of Mexico and the Caribbean Sea. Histological confirmation of the PCR results was not undertaken, and no haplosporidian has been reported from the numerous histological studies and surveys of oysters in the region. To further investigate the possibility that H. nelsoni is present in this region, we sampled 210 oysters from 40 sites around the Gulf of Mexico and Puerto Rico using PCR and 180 of these using tissue-section histology also. None of the oysters showed evidence of H. nelsoni by PCR or of any haplosporidian by histology. We cannot, therefore, confirm that H. nelsoni is present and widespread in the Gulf of Mexico and the Caribbean Sea. Our results do not prove that H. nelsoni is absent from the region, but taken together with results from previous histological surveys, they suggest that for the purposes of controlling oyster importation, the region should continue to be considered free of the parasite. KEY WORDS: PCR · Oyster · Crassostrea virginica · Molecular detection · Infection · Shellfish importation · Haplosporidian Resale or republication not permitted without written consent of the publisherDis Aquat Org 93: [251][252][253][254][255][256] 2011 C. virginica from Nova Scotia, Canada, to Biscayne Bay, Florida, USA (Haskin & Andrews 1988, Barber et al. 1997, Stephenson et al. 2003, but none of the several histological studies of oysters in the Gulf of Mexico have reported H. nelsoni or any other haplosporidian (Couch 1985, Gauthier et al. 1990, Fisher et al. 1996, Kim et al. 1998, Winstead et al. 2004, Kim & Powell 2006. The most comprehensive of these is the annual status and trends (S&T) MATERIALS AND METHODSCrassostrea virginica were collected between December 2007 and February 2008 at 32 sites representing 14 bay systems from lower Laguna Madre, Texas, to Florida Bay, Florida, USA, and C. rhizophorae were collected from 2 bays in Puerto Rico (Fig. 1, Table 1). In May and July 2010, C. virginica were sampled from 6 additional sites, representing 2 bay systems in Texas and 1 in Florida (Fig. 1, Table 1). All samples were express shipped directly to HSRL. Upon receipt, 12 oysters from each sample were opened using a shucking knife, which was rinsed in 10% bleach between each oyster. A section of gill about 4 mm wide and encompassing all 4 demibranchs approximately 6 to 7 mm posterior to the gill-palp junction was excised and preserved in 70% ethanol (EtOH) for PCR analysis. Dissecting instruments were scraped clean in a bleach-sand mixture, dipped in EtOH and flamed between each oyster. Between the PCR section and the palps, and adjacent to the piece of gill ta...

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Prospective study of infectious and noninfectious diseases in oysters and fishes in three Gulf of Mexico estuaries

Cited by 54 publications

References 29 publications

Effects of water-borne cadmium on the skin of the common carp (Cyprinus carpio)

Effects of water-borne cadmium on the skin of the common carp (Cyprinus carpio)

Histopathological changes related to chemical contamination in Mytilus edulis from field and experimental conditions

Widespread survey finds no evidence of Haplosporidium nelsoni (MSX) in Gulf of Mexico oysters

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