Prospective study of MMP7 serum levels in the diagnosis of cholangiocarcinoma

Leelawat, Kawin; Narong, Siriluck; Wannaprasert, Jerasak; Ratanashu-ek, Thawee

doi:10.3748/wjg.v16.i37.4697

Cited by 54 publications

(35 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…MMP-7 has also been shown to activate other MMPs, such as pro-MMP-2 and pro-MMP-9, and to inhibit E-cadherin function by inducing ectodomain shedding of E-cadherin (23,24). The results of several recent studies have indicated that MMP-7 is overexpressed in a variety of epithelial cancers, including esophagus, colon, pancreas, and cholangiocarcinoma, and is associated with cancer progression (25)(26)(27)(28). Thus, the invasive capacity of CD24 + cells not only depends on their adherent capabilities but also on increased MMP-7 expression.…”

Section: Discussionmentioning

confidence: 97%

Expression of CD24 in cholangiocarcinoma cells is associated with disease progression and reduced patient survival

Keeratichamroen

Leelawat

Thongtawee³

et al. 2011

Int J Oncol

Self Cite

View full text Add to dashboard Cite

Abstract. Cholangiocarcinoma is frequently found to invade local tissues and metastasize to distal organs. We investigated the expression of CD24 in cholangiocarcinoma samples and its prognostic significance. In addition, the cellular function of CD24 was studied in the RMCCA1 cholangiocarcinoma cell line. High CD24 expression significantly correlated with lymph node metastasis and positive surgical margins in cholangiocarcinoma patients. Univariate and multivariate analyses further demonstrated that CD24 expression was significantly associated with the overall survival of these patients (p=0.007 and p=0.040, respectively). For in vitro studies, the magnetic-activated cell sorting (MACS) system was used to isolate CD24 + and CD24 -cell populations from RMCCA1 cells. CD24 + RMCCA1 cells had increased chemoresistance, adhesion (p=0.004), motility (p<0.001), migration (p<0.001) and invasion (p<0.001) capabilities when compared to CD24 -cells. The matrix metalloproteinase (MMP)-7 was significantly elevated in CD24 + RMCCA1 cells (p=0.01). We found that inhibition of CD24 using siRNA silencing significantly decreased the invasive capacity of RMCCA1 cells. Both clinical and in vitro studies suggest that expression of CD24 is associated with cholangiocarcinoma disease progression. CD24 may thus serve as a new target for directed molecular therapy of cholangiocarcinoma. IntroductionCholangiocarcinoma is one of the most aggressive malignant tumors associated with local tissue invasion and a high rate of metastasis and is one of the most common causes of cancer death in Thailand (1). Three-year survival rates of 35-50% are achieved only in a subset of patients who have negative histological margins at the time of surgery (2-5). Palliative therapeutic approaches, including percutaneous and endoscopic biliary drainage, have generally been used for these patients because there is no effective chemotherapeutic treatment for cholangiocarcinoma. Therefore, identification of the molecules involved in cholangiocarcinoma cell progression is crucial for the development of novel drug treatments for this disease.CD24 is a small, heavily glycosylated mucin-like glycosylphosphatidylinositol (GPI)-linked cell surface protein that serves as a ligand for P-selectin, an adhesion receptor on activated endothelial cells and platelets (6). CD24 can be detected in granulocytes, pre-B-cells and keratinocytes (7) and its expression in non-small cell lung cancer, ovarian cancer and hepatocellular carcinoma is associated with poor prognoses (8,9). However, only limited studies have been published on CD24 expression in cholangiocarcinoma (10,11) and there are no data on the mechanism of CD24 involvement in cholangiocarcinoma cell progression. In the present study, we investigated the clinicopathological significance of CD24 expression in human cholangiocarcinoma samples. We also determined the cellular function of CD24 in cholangiocarcinoma cells by isolating CD24 + and CD24 -cells from the RMCCA1 cholangiocarcinoma cell line and assessing cell pro...

show abstract

Section: Discussionmentioning

confidence: 97%

Expression of CD24 in cholangiocarcinoma cells is associated with disease progression and reduced patient survival

Keeratichamroen

Leelawat

Thongtawee³

et al. 2011

Int J Oncol

Self Cite

View full text Add to dashboard Cite

show abstract

“…Serum levels of MMP-7 are also elevated in CCA patients and have been suggested as a diagnostic biomarker for CCA [40]. For the discrimination between CCA patients and healthy controls, a diagnostic sensitivity of 75% with a specificity of 78% was described [41]. …”

Section: Resultsmentioning

confidence: 99%

Circulating Biomarkers for Cholangiocarcinoma

Loosen

Vucur

Roderburg

et al. 2018

Dig Dis

View full text Add to dashboard Cite

Background: Cholangiocarcinoma (CCA) represents the second most common primary liver malignancy. The incidence rate has constantly increased over the last decades and CCA patients face a dismal prognosis with a 5-year survival rate of less than 5% for advanced stage of disease. Surgical tumor resection has remained the only potentially curative treatment option in daily practice but is often not feasible due to advanced disease stage at initial diagnosis. Summary: The early detection of cholangiocarcinoma is essential to provide patients with a potentially curative treatment. Furthermore, prognostic biomarkers represent a valuable tool to offer patients a tailored therapeutic approach in accordance to their life expectancy. The clinically most established biomarker carbohydrate antigen 19–9 shows only a limited diagnostic and prognostic power, encouraging the evaluation of novel biomarkers for cholangiocarcinoma in the last years. Key Massage: In this review, we assess currently available and potential future biomarkers for the diagnosis and prognosis of cholangicarcinoma.

show abstract

“…The strongest evidence for such activity has been in vitro experiments concerning matrilysin (MMP-7) Vargo-Gogola et al (2002). MMP-7 has been found to be over-expressed in several tumors, such as those associated with esophageal, cholangiocarcinoma, gastric, colon, prostate and bladder cancer (Hashimoto et al,1998;Martinez-Fernandez et al, 2009;Leelawat et al, 2010;Yeh et al, 2010;Szarvas et al, 2011;Zhou et al, 2011). MMP-7 levels are included in the evaluation of cancer invasion and metastases.…”

Section: Introductionmentioning

confidence: 99%

“…Degradation by MMP-9 is required for both tumor invasion and metastases (Murray et al, 2004). Increased invasion and expression of MMP-9 in human colorectal lines by a CD44-dependent mechanism (Hashimoto et al, 1998;Murray et al, 2004;Martinez-Fernandez et al, 2009;Leelawat et al, 2010;Yeh et al, 2010;Szarvas et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

Preoperative Levels of Matrix Metalloproteinase-7 and -9 and Tissue Inhibitor of Matrix Metalloproteinase-1 Relation to Pathologic Parameters in Bladder Carcinoma Patients

Güneş¹,

Kemik²,

Pirinççi³

et al. 2013

Asian Pacific Journal of Cancer Prevention

View full text Add to dashboard Cite

Our aim was to test the hypothesis that preoperative serum levels of matrix metalloproteinase-7 (MMP-7) and -9 (MMP-9) and tissue inhibitor of matrix metalloproteinase (TIMP-1) levels correlate with pathological features. Serum levels of MMP-7, and MMP-9 and TIMP-1 were determined in 90 bladder cancer patients and 40 healthy controls using an enzyme linked immunosorbent assay. Preoperative serum MMP-7 and MMP-9 levels were significantly higher in cancer patients than control groups (p<0.001). In contast, serum TIMP-1 levels were lower (p<0.001). Alteration in MMP-7, and MMP-9, and TIMP-1 production may contribute to tumor angiogenesis and be associated with clinic-pathological features.

show abstract

Prospective study of MMP7 serum levels in the diagnosis of cholangiocarcinoma

Abstract: Serum values of MMP7 and CA19-9 appear to be useful biomarkers for differentiating cholangiocarcinoma from benign biliary tract obstructive diseases.

Cited by 54 publications

References 27 publications

Expression of CD24 in cholangiocarcinoma cells is associated with disease progression and reduced patient survival

Expression of CD24 in cholangiocarcinoma cells is associated with disease progression and reduced patient survival

Circulating Biomarkers for Cholangiocarcinoma

Preoperative Levels of Matrix Metalloproteinase-7 and -9 and Tissue Inhibitor of Matrix Metalloproteinase-1 Relation to Pathologic Parameters in Bladder Carcinoma Patients

Contact Info

Product

Resources

About