Prospective study of SEVA TB peroxidase assay for cocktail antigen and antibody in the diagnosis of Tuberculosis in suspected patients attending a tertiary care hospital located in rural area

Majumdar, Anindita; Kamble, Pranita D; Badole, CM; Harinath, B. C.

doi:10.1016/s1995-7645(10)60086-7

Cited by 4 publications

(4 citation statements)

References 8 publications

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“…Similar observations were made for detection of antibody and antigen in patients of extra pulmonary tuberculosis [30,31]. In a prospective study, the in house developed SEVA TB ELISA using cocktail of ES-31 and EST-6 antigens showed 100% correlation (42 cases) with AFB positivity and ATT treatment [32]. The study further showed importance of ELISA with 80% positivity in AFB negative TB but clinically diagnosed and ATT treated cases [32,33].…”

Section: Diagnostically Important Excretory Secretory (Es) Proteinsmentioning

confidence: 61%

“…In a prospective study, the in house developed SEVA TB ELISA using cocktail of ES-31 and EST-6 antigens showed 100% correlation (42 cases) with AFB positivity and ATT treatment [32]. The study further showed importance of ELISA with 80% positivity in AFB negative TB but clinically diagnosed and ATT treated cases [32,33]. This can make an important contribution in confirming TB and correlating with clinical decision in smear negative TB cases in PTB and EPTB.…”

Section: Diagnostically Important Excretory Secretory (Es) Proteinsmentioning

confidence: 93%

“…A newly developed peroxidase enzyme immunoassay was explored using cocktail of ES-31, ES-43 and EST-6 antigens for antibody detection and affinity purified antibody for antigen detection in tuberculosis patients at Kasturba Hospital [33]. This assay on prospective analysis showed 100% correlation of PTB and EPTB acid fast bacilli positivity and anti TB treatment in 11 cases [32]. The same cocktails of antigens and antibodies were explored and found significant correlation (91/95) with ELISA negativity and no ATT advised to clinically suspected bone and joint and abdominal etc.…”

Section: Diagnostically Important Excretory Secretory (Es) Proteinsmentioning

confidence: 99%

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Excretory Secretory Proteins Released during Growth of Mycobacterium tuberculosis (H37Ra), With Diagnostic Potential in Pulmonary and Extra Pulmonary Tuberculosis

Waghmare¹,

Wankhade²,

Jena³

et al. 2016

Mycobact Dis

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TB immunodiagnostics based on antibody and antigen detection for early detection and monitoring tubercular infections at low cost and flexible to adapt to field laboratories are a boon to developing countries. In this context we have explored in-house developed penicillinase based ELISA assays for the detection of antigen, antibody as well as immune-complexed antigen using various excretory secretory antigenic proteins and specific antibodies. Excretory secretory protein antigens such as ES-31, ES-41, ES-43, ES-6, ES-20, ES-100 and EST-6 were studied extensively and found to be useful in various pulmonary and extra pulmonary cases of tuberculosis infection. ES-31 has shown its diagnostic potential in chronic PTB cases, ES-43 in relapse cases and ES-41 in bone and joint TB. Elevated level of ES-20 antigen was observed in patients with weak immune response in TB lymphadenitis. Detection of ES-100 antigen and antibody by penicilinase ELISA was observed to be useful in detection of TB meningitis. ES-6 antigen was shown to be useful in detection of latent infection. These proteins with antigenic properties are utilized for production of specific antibodies against them and observed to be useful in immune diagnostics for detection of specific circulating and immune complexed antigens. Further user friendly peroxidase immunoassay has been standardised and is being routinely used for suspected TB cases attending 1000 bedded Kasturba Hospital attached to medical institute on physician's request.

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Section: Diagnostically Important Excretory Secretory (Es) Proteinsmentioning

confidence: 61%

Section: Diagnostically Important Excretory Secretory (Es) Proteinsmentioning

confidence: 93%

Section: Diagnostically Important Excretory Secretory (Es) Proteinsmentioning

confidence: 99%

See 1 more Smart Citation

Excretory Secretory Proteins Released during Growth of Mycobacterium tuberculosis (H37Ra), With Diagnostic Potential in Pulmonary and Extra Pulmonary Tuberculosis

Waghmare¹,

Wankhade²,

Jena³

et al. 2016

Mycobact Dis

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show abstract

“…bacilli from M. chelonae, Nocardia farcinicum and Escherichia coli using Fluorescein isothiocyanate (FITC) conjugated anti-ES-31 antibody has been reported (12). A cocktail of affinity purified antibodies against ES-31, ES-43 and EST-6 antigens was shown to be useful for circulating antigen detection in TB by Penicillinase ELISA (13). Diagnostic potential of affinity purified antibodies against ES-20 antigen was also found useful in detection of tuberculosis cases (14,15).…”

Section: Introductionmentioning

confidence: 99%

Potential of mycobacterial excretory secretory protein antigens (sevatb ES-31, ES-43, EST-6 and ES-20) as biomarkers to detect Mycobacterium tuberculosis bacilli

Anindita

Thamke

Mendiratta

et al. 2010

Indian J Clin Biochem

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There is a need for a simple and reliable method to identify Mycobacterium tuberculosis from nontuberculous mycobacteria (NTM). The utility of mycobacterial ES-31, ES-43, EST-6 or ES-20 antigen as a biomarker for differentiation of Mycobacterium tuberculosis bacilli from nontuberculous mycobacteria was explored using Fluorescein isothiocyanate conjugated antibodies against these antigens. Detection of these antigens was done from M.tb H(37)Ra and H(37)Rv DSS antigen. The presence of antigen in bacilli using FITC labelled antibody was indicated by green fluorescence on the cell surface while, its absence by no fluorescence under microscope. In M.tb H(37)Ra and H(37)Rv bacilli, fluorescence was observed on addition of FITC labelled anti ES-31 and anti ES-43 antibody; whereas no fluorescence was observed in case of EST-6 and ES-20 antibody conjugates. However all the antigens were detected in detergent soluble sonicate antigen of tubercle bacilli on addition of FITC conjugates. Fluorescence was not observed for ES-31, ES-43, EST-6 and ES-20 antigen in any of the tested NTM as well as in Escherichia coli. SEVA TB ES-31 and ES-43 may be used as biomarkers to distinguish M.tuberculosis bacilli from NTM.

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Improved laboratory diagnosis of tuberculosis – The Indian experience

Haldar

Bose²,

Chakrabarti

et al. 2011

Tuberculosis

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Prospective study of SEVA TB peroxidase assay for cocktail antigen and antibody in the diagnosis of Tuberculosis in suspected patients attending a tertiary care hospital located in rural area

Cited by 4 publications

References 8 publications

Excretory Secretory Proteins Released during Growth of Mycobacterium tuberculosis (H37Ra), With Diagnostic Potential in Pulmonary and Extra Pulmonary Tuberculosis

Excretory Secretory Proteins Released during Growth of Mycobacterium tuberculosis (H37Ra), With Diagnostic Potential in Pulmonary and Extra Pulmonary Tuberculosis

Potential of mycobacterial excretory secretory protein antigens (sevatb ES-31, ES-43, EST-6 and ES-20) as biomarkers to detect Mycobacterium tuberculosis bacilli

Improved laboratory diagnosis of tuberculosis – The Indian experience

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