Prospective study of the duration and magnitude of viraemia in children hospitalised during the 1996-1997 dengue-2 outbreak in French Polynesia

Murgue, Bernadette; Roche, Claudine; Chungue, Eliane; Deparis, Xavier

doi:10.1002/(sici)1096-9071(200004)60:4<432::aid-jmv11>3.0.co;2-7

Cited by 132 publications

(105 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Previously, we found variation from subject to subject in DV-specific T cell responses after primary infection (41), but those subjects were not subsequently challenged with DV and there is no information about subsequent natural exposures to DV in that group. Recently, studies from our group and others have shown that plasma titers of infectious DV or DV RNA are higher in subjects with DHF than in those with DF (45)(46)(47). We have also found that immune activation, as measured by plasma levels of soluble TNF receptors, soluble IL-2 receptors, IFN-␥, and IL-10, correlated with peak plasma DV RNA levels and that the peak plasma DV RNA level was the only one of these factors independently associated with the severity of plasma leakage by multiple regression analysis (47).…”

Section: Discussionmentioning

confidence: 91%

T Cell Responses to an HLA-B*07-Restricted Epitope on the Dengue NS3 Protein Correlate with Disease Severity

Zivna

Green

Vaughn

et al. 2002

The Journal of Immunology

128

117

View full text Add to dashboard Cite

Dengue hemorrhagic fever (DHF), the severe manifestation of dengue virus (DV) infection characterized by plasma leakage, is more common in secondary DV infections in previously infected individuals and is associated with high levels of immune activation. To determine the Ag specificity of this immune response, we studied the response to an HLA-B*07-restricted T cell epitope, residues 221–232 of the DV NS3 protein, in 10 HLA-B*07+ Thai children who were studied during and after acute DV infections. Peptide-specific T cells were detected in 9 of 10 subjects. The frequency of peptide-specific T cells was higher in subjects who had experienced DHF than in those who had experienced DF. We also detected peptide-specific T cells in PBMC obtained at the time of the acute DV infection in 2 of 5 subjects. These data suggest that the NS3 (221–232) epitope is an important target of CD8+ T cells in secondary DV infection and that the activation and expansion of DV-specific T cells is greater in subjects with DHF than in those with dengue fever. These findings support the hypothesis that activation of DV-specific CD8+ T cells plays an important role in the pathogenesis of DHF.

show abstract

Section: Discussionmentioning

confidence: 91%

T Cell Responses to an HLA-B*07-Restricted Epitope on the Dengue NS3 Protein Correlate with Disease Severity

Zivna

Green

Vaughn

et al. 2002

The Journal of Immunology

128

117

View full text Add to dashboard Cite

show abstract

“…that 3.9% of patients were primary infections and the other 96.1% were re-infection cases [14,15]. These reports suggested that dengue infections in primary infections produced slight symptoms or were asymptomatic, while that in re-infection generated more acute symptoms [3,33]. It was considered that ADE could influence the increased risks of progress to a symptomatic state and increased transmission probability for re-infection.…”

Section: Figure 9 Figure 10mentioning

confidence: 99%

A dengue transmission model in Thailand considering sequential infections with all four serotypes

Chikaki

Ishikawa

2009

J Infect Dev Ctries

View full text Add to dashboard Cite

Background: Dengue fever/dengue haemorrhagic fever is prevalent in Thailand, where all serotypes are found and the dominant serotype has changed irregularly. Although almost all primary infections present with slight symptoms or are asymptomatic, little is known about the infectiousness of dengue fever. Methodology: A mathematical model of the transmission for dengue virus was constructed covering the possibility of sequential infections with all four different serotypes. The model was combined with the seasonal population dynamics of Aedes aegypti, the principal vectors of dengue virus in Thailand. The contributions of inapparent cases in the transmission to mosquito vectors and antibody-dependent enhancement were incorporated into the model. Moreover, the hypothesis of an "unnatural" infection route was examined, where a person acquires immunity by infection during a cross-immunity period, through model simulations. Results: A comparative study on the transmission probabilities of inapparent cases to mosquito vectors showed that the prevalence of dengue infection could be immediately stamped out after a severe outbreak if inapparent cases had no infectiousness. The simulation under an "unnatural" infection route assumption resulted of yearly changes in the dominant serotype and sharp, irregular variations in outbreaks. Conclusion: The supposition that inapparent cases had no infectiousness was not in accord with the actual situation in Thailand. Furthermore, the simulation result supported the "unnatural" infection route as having an influence on epidemics of dengue.

show abstract

“…It has long been assumed, but not empirically verified, that people with inapparent infections fail to infect mosquitoes because they do not reach sufficiently high viremia levels (5). This assumption is based on the observation that disease severity is positively correlated with the magnitude of DENV viremia (10,11,14). To our knowledge, the only study that quantified viral RNA levels in a limited number of asymptomatic DENV infections in humans did not detect a significantly lower viremia (15), but infectiousness to mosquitoes was not evaluated.…”

mentioning

confidence: 99%

“…aegypti fed on viremic people were able to transmit virus to another person after at least 11 d of extrinsic incubation (8). Results from later studies indicated that, for naturally infected people with clinically apparent dengue, the duration of detectable viremia was on average 4-5 d after the onset of symptoms, but could range from 2 to 12 d (9,10). Investigators in Vietnam fed Ae.…”

mentioning

confidence: 99%

Asymptomatic humans transmit dengue virus to mosquitoes

Duong

Lambrechts

Rácz

et al. 2015

Proc. Natl. Acad. Sci. U.S.A.

408

367

View full text Add to dashboard Cite

Three-quarters of the estimated 390 million dengue virus (DENV) infections each year are clinically inapparent. People with inapparent dengue virus infections are generally considered dead-end hosts for transmission because they do not reach sufficiently high viremia levels to infect mosquitoes. Here, we show that, despite their lower average level of viremia, asymptomatic people can be infectious to mosquitoes. Moreover, at a given level of viremia, DENV-infected people with no detectable symptoms or before the onset of symptoms are significantly more infectious to mosquitoes than people with symptomatic infections. Because DENV viremic people without clinical symptoms may be exposed to more mosquitoes through their undisrupted daily routines than sick people and represent the bulk of DENV infections, our data indicate that they have the potential to contribute significantly more to virus transmission to mosquitoes than previously recognized.mosquito experimental infection | Cambodia | Aedes aegypti | human-to-mosquito transmission | dengue W ith 3.97 billion people living in 128 countries currently at risk for infection, dengue viruses (DENV-1 to -4) cause more human morbidity and mortality worldwide than any other arthropod-borne virus (1, 2). Aedes aegypti mosquitoes are the primary vectors of DENV throughout the tropics (3). Dengue prevention relies on the control of Ae. aegypti populations, which is failing in most parts of the world due to lack of resources, lack of political will, and/or ineffective implementation (4).Virus transmission from infected humans to mosquitoes is a critical step in dengue epidemiology, but due to logistical constraints it has been directly examined only in a handful of studies to date (5). In initial experimental infections of human volunteers during the 1920s (6, 7), the onset of clinical symptoms occurred 4-9 d after virus inoculation by mosquito bite (8). DENV-infected humans were infectious to mosquitoes from 2 d before to 2 d after the onset of symptoms, and Ae. aegypti fed on viremic people were able to transmit virus to another person after at least 11 d of extrinsic incubation (8). Results from later studies indicated that, for naturally infected people with clinically apparent dengue, the duration of detectable viremia was on average 4-5 d after the onset of symptoms, but could range from 2 to 12 d (9, 10). Investigators in Vietnam fed Ae. aegypti directly on 208 symptomatic, hospitalized dengue patients and reported that the probability of successful human-to-mosquito DENV transmission was coincident with the kinetics of viremia (11). Dengue patients were infectious up to 5 d after the onset of symptoms, which generally corresponded with "defervescence" (11).All previous studies on human-to-mosquito DENV transmission were limited to people with overt illness and did not consider subclinical infections. An estimated 300 million of the total 390 million DENV infections per year are clinically inapparent or mildly symptomatic, i.e., no illness that disrupted a person's ...

show abstract

Prospective study of the duration and magnitude of viraemia in children hospitalised during the 1996-1997 dengue-2 outbreak in French Polynesia

Cited by 132 publications

References 33 publications

T Cell Responses to an HLA-B*07-Restricted Epitope on the Dengue NS3 Protein Correlate with Disease Severity

T Cell Responses to an HLA-B*07-Restricted Epitope on the Dengue NS3 Protein Correlate with Disease Severity

A dengue transmission model in Thailand considering sequential infections with all four serotypes

Asymptomatic humans transmit dengue virus to mosquitoes

Contact Info

Product

Resources

About