Prospective study of the enzymatic activities in urine of n-acetyl-β-d-glucosaminidase, α-d-mannosidase, α- and β-d-glucosidases, α-l- and β-d-fucosidases, and β-d-galactosidase in type I diabetes mellitus with early nephropathy

Miralles, José Manuel Pavía; Velasco, Javier; Villabona, Vicente; Sánchez-Bernal, Carmen; Pérez, Nieves; Corrales, J. J.; García-Díez, L C; Villar, Enrique

doi:10.1016/1056-8727(93)90045-z

Cited by 8 publications

(4 citation statements)

References 35 publications

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“…36 However, no significant correlation has been found between the examined enzyme activity of GAL and the level of albuminuria with T1DM in a prospective study. 37 Besides, GAL did not show a significant impact on albuminuria when adjusted by PSM method in our study. NAG, as a sensitive marker of MA, is found in patients with T2DM, 38 and the regression of MA in T1DM was associated with the lower NAG level at baseline in a cross-sectional study.…”

Section: Discussioncontrasting

confidence: 50%

Evaluation of urinary biomarkers for prediction of diabetic kidney disease: a propensity score matching analysis

Qin

Zhang

Shen

et al. 2019

Therapeutic Advances in Endocrinology

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Background: The aim of this study was to evaluate the diagnostic value of six urinary biomarkers for prediction of diabetic kidney disease (DKD). Methods: The cross-sectional study recruited 1053 hospitalized patients with type 2 diabetes mellitus (T2DM), who were categorized into the diabetes mellitus (DM) with normoalbuminuria (NA) group ( n = 753) and DKD group ( n = 300) according to 24-h urinary albumin excretion rate (24-h UAE). Data on the levels of six studied urinary biomarkers [transferrin (TF), immunoglobulin G (IgG), retinol-binding protein (RBP), β-galactosidase (GAL), N-acetyl-beta-glucosaminidase (NAG), and β2-microglobulin (β2MG)] were obtained. The propensity score matching (PSM) method was applied to eliminate the influences of confounding variables. Results: Patients with DKD had higher levels of all six urinary biomarkers. All indicators demonstrated significantly increased risk of DKD, except for GAL and β2MG. Single RBP yielded the greatest area under the curve (AUC) value of 0.920 compared with the other five markers, followed by TF (0.867) and IgG (0.867). However, GAL, NAG, and β2MG were shown to have a weak prognostic ability. The diagnostic values of the different combinations were not superior to the single RBP. Conclusions: RBP, TF, and IgG could be used as reliable or good predictors of DKD. The combined use of these biomarkers did not improve DKD detection.

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Section: Discussioncontrasting

confidence: 50%

Evaluation of urinary biomarkers for prediction of diabetic kidney disease: a propensity score matching analysis

Qin

Zhang

Shen

et al. 2019

Therapeutic Advances in Endocrinology

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“…More data are available with respect to urinary NAG in DM (especially type 1 DM) [21][22][23][24], albeit mostly on random or timed urine samples. An increased excretion of low molecular weight proteins and enzymes in the urine, including urinary NAG, occurs very early during the diabetic process, even predating the appearance of microal-buminuria [22][23][24].…”

Section: Discussionmentioning

confidence: 99%

Proteinuria and Renal Tubular Damage: Urinary N-Acetyl- β-D-Glucosaminidase and Isoenzymes in Dissimilar Renal Disease

Rustom¹,

Costigan

Shenkin

et al. 1998

Am J Nephrol

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Markers of renal tubular injury are difficult to interpret in patients with proteinura. The 24-hour urinary N-acetyl-β-D-glucosaminidase (NAG) concentration was measured in 167 patients with dissimilar renal disease, function, and proteinuria. NAG isoenzymes were also separated in 69 patients, using a modified fast protein liquid chromatography technique. The ‘A2’ isoenzyme predominated at all levels of renal function and in all diagnostic groups. Urinary NAG and proteinuria were well correlated at all levels of renal function, as was NAG ‘A2’ isoenzyme. Proteinuria and urinary NAG were similarly correlated in patients with different glomerulonephritides, hypertensive nephrosclerosis, and chronic pyelonephritis, but not in those with diabetic nephropathy.

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“…The mean urinary NAG activity in normal controls was 2.9 ± 1.2 U/g Cr (Fig. 3) , and the upper limit was Although increased urinary NAG excretion in diabetes has previously been shown [25,[44][45][46][47] , we did not find a significant difference between diabetics and controls (p = 0 .052) whereas mean urinary activity was higher in diabetics than in controls. This is partly due to the age of our subjects (40-49 years In summary, urinary DAP N was higher in NIDDM subjects without clinical proteinuria but NAG was not,…”

Section: Methodsmentioning

confidence: 49%

Urinary dipeptidyl aminopeptidase IV as a marker of early tubular damage in diabetic nephropathy

Nagata

Umeda

Urakami

et al. 1999

The Journal of Kansai Medical University

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The aim of this study was to assess the role of urinary dipeptidyl aminopeptidase N (DAP IV) in early diabetic nephropathy and impaired glucose tolerance (IGT). We compared urinary DAP N activity, N-acetyl-b-D-glucosaminidase (NAG) activity, and albumin levels between healthy subjects, non-insulin-dependent diabetic (NIDDM) subjects that did not show clinical proteinuria, and IGT subjects. In healthy subjects, a significant difference in the excretion of urinary DAP IV occurred with age. Therefore, we focused mainly on the results of the forties of each group. Urinary DAP IV activity in NIDDM subjects, and IGT subjects was significantly higher than that in controls (1 1.8 ± 5.3, 7.2 ± 1.4, 5.7 ± 0.8 U/g creatinine (Cr) , respectively, p < 0.0 0 1). No statistically significant difference in urinary NAG activity was observed compared with controls (NIDDM; 4.5 ± 2.7, IGT; 3.7 ± 1.7, control; 2.9 ± 1.2 U/g Cr). A significant correlation was observed between urinary DAP N and urinary NAG in NIDDM and IGT groups. In both groups, some subjects showed elevated DAP N activity despite normal NAG activity, while no subjects showed both normal DAP IV activity and higher NAG activity. Urinary albumin levels in NIDDM subjects also were higher than those in controls (p < 0.0 1), but this difference was less pronounced than that for DAP IV activity (p < 0.0 0 1). We conclude that measurement of urinary DAP N activity is a useful tool for detecting early diabetic nephropathy.

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Prospective study of the enzymatic activities in urine of n-acetyl-β-d-glucosaminidase, α-d-mannosidase, α- and β-d-glucosidases, α-l- and β-d-fucosidases, and β-d-galactosidase in type I diabetes mellitus with early nephropathy

Cited by 8 publications

References 35 publications

Evaluation of urinary biomarkers for prediction of diabetic kidney disease: a propensity score matching analysis

Evaluation of urinary biomarkers for prediction of diabetic kidney disease: a propensity score matching analysis

Proteinuria and Renal Tubular Damage: Urinary N-Acetyl- β-D-Glucosaminidase and Isoenzymes in Dissimilar Renal Disease

Urinary dipeptidyl aminopeptidase IV as a marker of early tubular damage in diabetic nephropathy

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