Prospective study of urinary excretion of 7‐methylguanine and the risk of lung cancer: Effect modification by <i>mu</i> class glutathione‐<i>S</i>‐transferases

Loft, Steffen; Svoboda, Peter; Kasai, Hiroshi; Tjønneland, Anne; Möller, Peter; Sørensen, Mette; Overvad, Kim; Autrup, Herman; Raaschou-Nielsen, Ole

doi:10.1002/ijc.22863

Cited by 33 publications

(23 citation statements)

References 48 publications

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“…Lifestyle has a more significant effect on urinary m 7 Gua than 8-OH-dG, based on all statistical analyses. The urinary excretion of m 7 Gua has not been extensively investigated as a biomarker, (58) except for the influences of smoking. (29,54) In this study, urine samples from male working subjects at a specific company were analyzed.…”

Section: Discussionmentioning

confidence: 99%

Effect of age, smoking and other lifestyle factors on urinary 7‐methylguanine and 8‐hydroxydeoxyguanosine

Tamae¹,

Kawai²,

Yamasaki³

et al. 2009

Cancer Science

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Urinary 8-hydroxydeoxyguanosine (8-OH-dG) and 7-methylguanine (m 7 Gua) were measured by a column-switching high performance liquid chromatography method as markers of oxidative and methylating DNA damage, respectively. We investigated the associations between urinary 8-OH-dG or m 7 Gua and various lifestyle and demographic factors, such as age and sex. The urinary 8-OH-dG excretion level was positively correlated with cigarette smoking, but inversely correlated with fruit consumption, physical activity and total energy consumed per day. A multiple regression analysis revealed that daily physical activity and healthy meal combinations decreased the urinary 8-OH-dG level, whereas alcohol consumption increased it. In terms of the urinary m 7 Gua measurement, cigarette smoking, age and consumption of meat, fish, egg, soybean, etc. were positively correlated with the urinary m 7 Gua level, whereas body weight, BMI, physical activity, and dietary index score, which indicates good nutritional balance, were negatively correlated with the amount of m 7 Gua. Based on a multiple regression analysis, cigarette smoking and age correlated with the m 7 Gua level, while high BMI and healthy meal combinations have significant reducing effects on m 7 Gua level. Therefore, the urinary m 7 Gua level is considered to be a useful marker of DNA methylation, not only from smoking, but also from aging and unhealthy dietary habits. (Cancer Sci 2009; 100: 715-721) O xygen radicals are formed in cells by oxygen metabolism and various environmental agents, and they damage DNA, RNA, and proteins.(1) Among the many types of oxidative DNA damage, 8-hydroxydeoxyguanosine (8-OH-dG) is a major product and is frequently analyzed as a marker of cellular oxidative stress related to carcinogenesis, (2,3) because 8-OH-dG induces mutations, (4,5) is excreted in the urine, and it has been analyzed by high performance liquid chromatography-electrochemical detection (HPLC-ECD), (6,7) liquid chromatography-tandem mass spectrometry (LC-MS), (8) gas chromatography-mass spectrometry (GC-MS),and enzyme linked immunosorbent assay (ELISA).(10) However, the reproducibility and accuracy of its measurement are much higher with the HPLC-ECD and LC-MS/MS methods, as compared to the ELISA method. (11,12) We have reported that higher 8-OH-dG levels were observed in the lung DNA of smokers, (13) the liver DNA of chronic hepatitis patients, (14) and in the stomach DNA of patients infected with Helicobacter pylori.(15) It has also been reported that the urinary 8-OH-dG level is higher in cancer patients than in healthy people, (16) higher in smokers than in nonsmokers, (17) and lower in people who exercise moderately. (17) In addition, the urinary 8-OH-dG level was higher in men than in women, (7) and it negatively correlated to body mass index (BMI).(7) As an explanation for the relationship between a lean BMI and high urinary 8-OH-dG excretion, it has been suggested that lean persons have a higher metabolic rate than obese persons, (18) and therefore have higher oxidat...

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Section: Discussionmentioning

confidence: 99%

Effect of age, smoking and other lifestyle factors on urinary 7‐methylguanine and 8‐hydroxydeoxyguanosine

Tamae¹,

Kawai²,

Yamasaki³

et al. 2009

Cancer Science

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“…Mutagenic changes in DNA sequences and epigenetic modifications can cause an incorrect DNA replication and transcription, and this is well known to be linked with several types of diseases and metabolic disorders [2][3][4]. Epigenetic modifications are defined as covalent chemical changes in DNA nucleic bases with no sequence modifications.…”

Section: Introductionmentioning

confidence: 99%

Boron-doped diamond electrodes explored for the electroanalytical detection of 7-methylguanine and applied for its sensing within urine samples

Sanjuán

Brotons

Hernández-Ibáñez

et al. 2016

Electrochimica Acta

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Please cite this article as: Ignacio Sanjuán, Ariadna Brotons, Naiara Hernández-Ibáñez, Christopher W.Foster, Craig E.Banks, Jesús Iniesta, Boron-doped diamond electrodes explored for the electroanalytical detection of 7-methylguanine and applied for its sensing within urine samples, Electrochimica Acta http://dx.doi.org/10.1016/j.electacta. 2015.11.026 This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.  SWV provides good resolution of the anodic peak for 7-mG and the main nucleic bases interferences, such as adenine and guanine. It is plausible the simultaneous determination of 7-mG and another of the most common modified nucleobases, 8-oxoguanine (8-oxoG). 7-mG electrochemical determination within a human urine sample is feasible with linear regression between peak current intensity versus 7-mG concentration. 3 AbstractEpigenetic modifications have been associated by many studies with several types of diseases and metabolic dysfunctions. Specifically, N7-methyl modification of guanine (7-mG) is well established to be used as a biomarker for the detection and determination of DNA methylation. The use of an electrochemical sensor has the potential to provide a simpler and more economic sensing methodology for the determination of 7-mG compared to traditionally utilised laboratory based approaches. In this paper we demonstrate the feasibility of an electrochemical sensor which could potentially be easily applied towards the determination of 7-mG within biological samples, such as human urine. A practical electrochemical configuration was employed consisting of a boron-doped diamond electrode (BDD) as the working electrode and a screen-printed graphite electrode (SPE) providing the counter electrode and the reference electrode.With this new protocol, the electrochemical behaviour of 7-mG has been investigated via cyclic voltammetry (CV) and square wave voltammetry (SWV) using a BDD electrode with a simplified electrochemical set-up The electrochemical behaviour of 7-mG within acetate buffer solutions at a BDD electrode has been compared and contrasted to a glassy carbon electrode with the following parameters studied: voltammetric scan rate, solution pH, 7-mG concentration and electrode surface pretreatment. The oxidative mechanism elucidation has been performed at controlled potential and such results have provided the dimer formation as the major product. The simultaneous electroanalytical identification of 7-mG together with the presence of guanine, adenine and 8-oxoguanine has been investigated under the optimum experimental conditions. Furthermore, the feasibility of using a BDD electrode for the ...

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“…If not repaired, apurinic sites can potentially cause G to T transversions (14). Previously, urinary excretion of N7-MeG has been found to be increased in laboratory animals after exposure to methylating agents (15), and the N7-MeG in urine has been shown to be higher among smokers than among nonsmokers, probably resulting from exposure to tobaccospecific nitrosamines (16,17). Although nitrosamines do exist in coke oven emissions, no information was available on the urinary N7-MeG levels of workers related to the occupational exposure to coke oven emissions.…”

Section: Introductionmentioning

confidence: 99%

Repeated Measurements of Urinary Methylated/Oxidative DNA Lesions, Acute Toxicity, and Mutagenicity in Coke Oven Workers

Chao¹,

Wang

et al. 2008

Cancer Epidemiology, Biomarkers &Amp; Prevention

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We conducted a repeated-measures cohort study of coke oven workers to evaluate the relationships between the traditional exposure biomarker, urinary 1-hydroxypyrene (1-OHP), and a series of biomarkers, including urinary 8-oxo-7,8-dihydro-2 ¶-deoxyguanosine (8-oxodG), N7-methylguanine (N7-MeG), acute toxicity, and mutagenicity. A total of eight spot urine samples were collected from each high-exposed (at topside oven area) and low-exposed workers (at side oven area) during the whole working cycle, which consisted of 6 consecutive days of working followed by 2 days off. Our results showed that the high-exposed workers had significantly higher urinary levels of 1-OHP, 8-oxodG, and N7-MeG compared with the low-exposed workers. Acute toxicity and mutagenicity of urine were also found to be markedly increased in the high-exposed workers, as determined by Microtox assay and Ames test, respectively. Multivariate regressions analysis revealed that the urinary 8-oxodG, N7-MeG, or acute toxicity was significantly correlated with 1-OHP concentrations. Overall, the present study showed that exposure to coke oven emissions increased oxidatively damaged DNA products and mutagenicity of urine, and for the very first time, such exposure was also found to increase DNA methylation and urinary acute toxicity. The potential source of methylating agents in coke oven emissions warrants further investigation. Additionally, with repeated measurements, the pattern of time course for urinary 1-OHP was found to be different from those of 8-oxodG and N7-MeG, as well as acute toxicity and mutagenicity. This finding implies that the single measurement that was often conducted in occupational healthy investigations should be used with certain precautions, because single measurement may fail to provide the proper information of interest. (Cancer Epidemiol Biomarkers Prev 2008;17(12):3381 -9)

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Prospective study of urinary excretion of 7‐methylguanine and the risk of lung cancer: Effect modification by mu class glutathione‐S‐transferases

Cited by 33 publications

References 48 publications

Effect of age, smoking and other lifestyle factors on urinary 7‐methylguanine and 8‐hydroxydeoxyguanosine

Effect of age, smoking and other lifestyle factors on urinary 7‐methylguanine and 8‐hydroxydeoxyguanosine

Boron-doped diamond electrodes explored for the electroanalytical detection of 7-methylguanine and applied for its sensing within urine samples

Repeated Measurements of Urinary Methylated/Oxidative DNA Lesions, Acute Toxicity, and Mutagenicity in Coke Oven Workers

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