Prospects and Pitfalls of Clonal Fishes in the Postgenomic Era

Robison, Barrie D.; Thorgaard, Gary H.

doi:10.1002/9780470963159.ch4

Cited by 1 publication

(2 citation statements)

References 56 publications

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“…The first is due to the gynogenetic technique itself, often called the treatment effect. 41 The fish used in this study were many generations removed from the parental descendent of the C32 line, and therefore, treatment effect cannot be considered a factor in this study. Reduced fitness of isogenic homozygous fish can also be due to the fixation of a sub-lethal allele as a result of the cloning process.…”

Section: Variability Of Performance Measured In Ihc and Wt Zebrafishmentioning

confidence: 99%

“…Reduced fitness of isogenic homozygous fish can also be due to the fixation of a sub-lethal allele as a result of the cloning process. 41 However, as previously discussed, the C32 line was selected for favorable laboratory conditions, and the lower hatching scores may just be due to strain differences among zebrafish.…”

Section: Variability Of Performance Measured In Ihc and Wt Zebrafishmentioning

confidence: 99%

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A Comparison of Isogenic Homozygous Clone and Wildtype Zebrafish (Danio rerio): Survival and Developmental Responses to Low pH Conditions

et al. 2020

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The value of bioassays as analytical methods for assessing the potency of particular stressors on live animal models depends on the precision of their results, which are greatly influenced by the choice of test subjects. The genetic makeup of experimental subjects varies, and, as such, so will their responses to the test environment. Genetic diversity of test populations may contribute to statistical variability; therefore, the use of genetically similar subjects may enhance the utility of bioassays. This study addresses the efficacy of using isogenic homozygous zebrafish (Danio rerio) as subjects for bioassays. Stress responses (acidic conditions) were compared during early development for gynogenetically produced isogenic homozygous line of zebrafish (C32) and wildtype (WT) zebrafish. Experiments evaluated early life stage milestones after exposure to low pH in water of a different electrolyte composition. Because the isogenic homozygous clonal (IHC) fish possessed far less genetic variability than the WT fish tested, it was predicted that the IHC fish would exhibit less variability in their response to stress. Although we found no significant differences in the variability between the responses of the IHC and WT fish, pH and water hardness level had a differential effect on the two groups. Simple strain differences may be the probable cause of the response differences to environmental stress. Factors that may affect stress response, such as heterogeneity, co-adapted gene complexes, and domestication, are discussed. Our findings and review of recent zebrafish literature stress the need for researchers to carefully consider breeding histories and trait characteristics for each potential test subject to maximize the sensitivity of the assay.

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Section: Variability Of Performance Measured In Ihc and Wt Zebrafishmentioning

confidence: 99%

Section: Variability Of Performance Measured In Ihc and Wt Zebrafishmentioning

confidence: 99%

A Comparison of Isogenic Homozygous Clone and Wildtype Zebrafish (Danio rerio): Survival and Developmental Responses to Low pH Conditions

et al. 2020

View full text Add to dashboard Cite

show abstract

Prospects and Pitfalls of Clonal Fishes in the Postgenomic Era

Cited by 1 publication

References 56 publications

A Comparison of Isogenic Homozygous Clone and Wildtype Zebrafish (Danio rerio): Survival and Developmental Responses to Low pH Conditions

A Comparison of Isogenic Homozygous Clone and Wildtype Zebrafish (Danio rerio): Survival and Developmental Responses to Low pH Conditions

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