Prospects for Bio‐Industrial Application of an Extremely Alkaline Mannanase From<i>Bacillus subtilis</i>subsp.<i>inaquosorum</i>CSB31

Regmi, Sudip; Yoo, Hah Young; Choi, Yun Hee; Choi, Yoon Seok; Yoo, Jin Cheol; Kim, Seung Wook

doi:10.1002/biot.201700113

Cited by 31 publications

(23 citation statements)

References 40 publications

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“…CS428 (pH 12.5) [ 22 ], B. subtilis subsp. inaquosorum CSB31 (pH 12.5) [ 53 ], B. halodurans PPKS-2 (pH 11.0) [ 49 ], Bacillus sp. JAMB-750 (pH 10.0) [ 50 ] and B. cereus N1 (pH 10.0) [ 54 ].…”

Section: Resultsmentioning

confidence: 99%

“…An extremely alkaline mannanase (MnB31) from B. subtilis subsp. inaquosorum CSB31 was found to have the highest optimal pH of 12.5 and to be stable at pH range from 5.8 to 12.5, which was the most alkaline β-mannanase reported to date [ 53 ]. In addition, almost all the reported Bacillus β-mannanases displayed optimal pH at the range from 6.0 to 12.5 (Table 2 ), which indicated that Bacillus strains were the main sources of neutral and alkaline β-mannanases.…”

Section: Resultsmentioning

confidence: 99%

“…Although the K m value of BcManA for the standard substrate LGB was higher than that of some β-mannanases from B. subtili s subsp. inaquosorum CSB31 (0.043 g l −1 ) [ 53 ], Bacillus sp. CSB39 (0.082 g l −1 ) [ 68 ], Bacillus sp.…”

Section: Resultsmentioning

confidence: 99%

“…MG-33 [ 69 ] and B. subtilis 5H [ 72 ], but different from those from B. subtilis WY34 [ 67 ] and B. subtilis subsp. inaquosorum CSB31 [ 53 ] which did not produce mannose.

Fig.…”

Section: Resultsmentioning

confidence: 99%

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Characterization and high-efficiency secreted expression in Bacillus subtilis of a thermo-alkaline β-mannanase from an alkaliphilic Bacillus clausii strain S10

Zhou

Xue

2018

Microb Cell Fact

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Backgroundβ-Mannanase catalyzes the cleavage of β-1,4-linked internal linkages of mannan backbone randomly to produce new chain ends. Alkaline and thermostable β-mannanases provide obvious advantages for many applications in biobleaching of pulp and paper, detergent industry, oil grilling operation and enzymatic production of mannooligosaccharides. However, only a few of them are commercially exploited as wild or recombinant enzymes, and none heterologous and secretory expression of alkaline β-mannanase in Bacillus subtilis expression system was reported. Alkaliphilic Bacillus clausii S10 showed high β-mannanase activity at alkaline condition. In this study, this β-mannanase was cloned, purified and characterized. The high-level secretory expression in B. subtilis was also studied.ResultsA thermo-alkaline β-mannanase (BcManA) gene encoding a 317-amino acid protein from alkaliphilic Bacillus clausii strain was cloned and expressed in Escherichia coli. The purified mature BcManA exhibited maximum activity at pH 9.5 and 75 °C with good stability at pH 7.0–11.5 and below 80 °C. BcManA demonstrated high cleavage capability on polysaccharides containing β-1,4-mannosidic linkages, such as konjac glucomannan, locust bean gum, guar gum and sesbania gum. The highest specific activity of 2366.2 U mg−1 was observed on konjac glucomannan with the Km and kcat value of 0.62 g l−1 and 1238.9 s−1, respectively. The hydrolysis products were mainly oligosaccharides with a higher degree of polymerization than biose. BcManA also cleaved manno-oligosaccharides with polymerization degree more than 3 without transglycosylation. Furthermore, six signal peptides and two strong promoters were used for efficiently secreted expression optimization in B. subtilis WB600 and the highest extracellular activity of 2374 U ml−1 with secretory rate of 98.5% was obtained using SPlipA and P43 after 72 h cultivation in 2 × SR medium. By medium optimization using cheap nitrogen and carbon source of peanut meal and glucose, the extracellular activity reached 6041 U ml−1 after 72 h cultivation with 6% inoculum size by shake flask fermentation.ConclusionsThe thermo-alkaline β-mannanase BcManA showed good thermal and pH stability and high catalytic efficiency towards konjac glucomannan and locust bean gum, which distinguished from other reported β-mannanases and was a promising thermo-alkaline β-mannanase for potential industrial application. The extracellular BcManA yield of 6041 U ml−1, which was to date the highest reported yield by flask shake, was obtained in B. subtilis with constitutive expression vector. This is the first report for secretory expression of alkaline β-mannanase in B. subtilis protein expression system, which would significantly cut down the production cost of this enzyme. Also this research would be helpful for secretory expression of other β-mannanases in B. subtilis.Electronic supplementary materialThe online version of this article (10.1186/s12934-018-0973-0) contains supplementary material, which is available to authorized users.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

“…MG-33 [ 69 ] and B. subtilis 5H [ 72 ], but different from those from B. subtilis WY34 [ 67 ] and B. subtilis subsp. inaquosorum CSB31 [ 53 ] which did not produce mannose.

Fig.…”

Section: Resultsmentioning

confidence: 99%

See 2 more Smart Citations

Characterization and high-efficiency secreted expression in Bacillus subtilis of a thermo-alkaline β-mannanase from an alkaliphilic Bacillus clausii strain S10

Zhou

Xue

2018

Microb Cell Fact

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“…inaquosorum CSB31," [6] the authors screen for a mannanase producer. An extreme alkaline mannanase (MnB31) enzyme was then purified and the biochemical and thermodynamic properties were examined for utilization in various biotechnological processes using different substrates.…”

mentioning

confidence: 99%

AFOB Special Issue on Industrial Biotechnology

Kim

Punnapayak

2017

Biotechnology Journal

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This Special issue of Biotechnology Journal (BTJ) industrial biotechnology, also called white biotechnology, which covers the technology for the production of biofuels, biochemicals, enzymes, biopharmaceuticals, as well as all involved bioprocesses. Industrial biotechnology can also be described as the manipulation of enzymes and microorganisms to produce energy, industrial chemicals, and consumer goods through renewable resources of plant-derived carbohydrates and other compounds. [1] It can reduce the reliance on fossil fuels and yield cost effective and sustainable industrial processes.We have witnessed an enormous development of this field, due to promising approaches to address the most challenging issues in our society, including climate change, resource conservation, cost reduction, and sustainability. [2] Advancement in the fields of industrial biotechnology will influence the world more than we can anticipate now. Through the use of natural resources and the development and manipulation of organisms, industrial biotechnology may provide us with new products and processes which seemed impossible in the past. It may also enable the industry to reduce cost and create new market opportunities while protecting the environment. [3] In addition, with this Special issue Biotechnology Journal continues its cooperation with the Asian Federation of Biotechnology (AFOB). Here are some highlight articles from this issue:A Research Article on "Formate and nitrate utilization in Enterobacter aerogenes for semi-anaerobic production of isobutanol" [4] reports on the engineering of isobutanol-producing E. aerogenes to increase its fitness via augmentation of the formate and nitrate metabolism during anaerobic cultivation. This was achieved by deletion of genes within the formate utilization pathway.Furthermore, another Research Article discusses the "Physiological and metabolomic analysis of Issatchenkia orientalis MTY1 with multiple tolerance for cellulosic bioethanol production." [5] Here, the alcohol producing yeast Issatchenkia orientalis MTY1 was isolated in a Korean winery and its tolerance against high temperature and acidic conditions was characterized in microaerobic batch cultures by metabolomic analysis.In the Research Article on "Prospects for bio-industrial application of an extremely alkaline mannanase from Bacillus subtilis subsp. inaquosorum CSB31," [6] the authors screen for a mannanase producer. An extreme alkaline mannanase (MnB31) enzyme was then purified and the biochemical and thermodynamic properties were examined for utilization in various biotechnological processes using different substrates.Moreover, a Research article on the "Bio-based production of dimethyl itaconate from rice wine waste-derived itaconic acid," [7] shows the potential for bio-based production of raw materials for copolymerization from renewable carbon sources. The authors demonstrate that dimethyl itaconate can be produced by engineered C. glutamicum, starting from itaconic acid produced from rice wine waste-derived carbohydra...

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Combinatorial Fine-Tuning of GNA1 and GlmS Expression by 5’-Terminus Fusion Engineering Leads to Overproduction of N-Acetylglucosamine inBacillus subtilis

Liu

Wang

et al. 2018

Biotechnol. J.

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Glucosamine-6-phosphate N-acetyltransferase (GNA1) that catalyzes acetyl transfer from acetyl-coenzyme A to glucosamine-6-phosphate (GlcN-6P), and glutamine-fructose-6-phosphate aminotransferase (GlmS) that catalyzes the formation of GlcN-6P from fructose-6-phosphate (Fru-6P), are two key enzymes in Bacillus subtilis for the bioproduction of N-acetylglucosamine (GlcNAc), a nutraceutical that has various applications in healthcare. In this study, the expression of GNA1 and GlmS is fine-tuned by 5'-terminus fusion engineering to improve GlcNAc production. Specifically, the expression level of GNA1 is enhanced at the translational level via fusion of an epitope tag to the 5'-terminus of GNA1 gene and ribosome binding site (RBS) sequence engineering. Next, enhanced expression of GlmS is achieved at the transcriptional and translational levels by fusing an mRNA stabilizer to the 5'-terminus of GlmS gene. Under the control of GNA1 (fusion with cMyc tag and with the optimum RBS M-Rm) and GlmS (fusion with mRNA stabilizer ΔermC+14/7A), the GlcNAc titer and yield in the shake flask increase to 18.5 g L and 0.37 g GlcNAc/g glucose, which are 2.9-fold and 2.3-fold that of the control, respectively. This synthetic pathway fine-tuning method at the transcriptional and translational levels by combinatorial modulation of regulatory elements, including epitope tag, RBS sequence, and mRNA stabilizer, might represent a general and effective approach for the construction of microbial cell factories.

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Prospects for Bio‐Industrial Application of an Extremely Alkaline Mannanase FromBacillus subtilissubsp.inaquosorumCSB31

Cited by 31 publications

References 40 publications

Characterization and high-efficiency secreted expression in Bacillus subtilis of a thermo-alkaline β-mannanase from an alkaliphilic Bacillus clausii strain S10

Characterization and high-efficiency secreted expression in Bacillus subtilis of a thermo-alkaline β-mannanase from an alkaliphilic Bacillus clausii strain S10

AFOB Special Issue on Industrial Biotechnology

Combinatorial Fine-Tuning of GNA1 and GlmS Expression by 5’-Terminus Fusion Engineering Leads to Overproduction of N-Acetylglucosamine inBacillus subtilis

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