We present observations of fifty square degrees of the Mopra carbon monoxide (CO) survey of the Southern Galactic Plane, covering Galactic longitudes l = 300-350 • and latitudes |b| ≤ 0.5 • . These data have been taken at 0.6 arcminute spatial resolution and 0.1 km s −1 spectral resolution, providing an unprecedented view of the molecular clouds and gas of the Southern Galactic Plane in the 109-115 GHz J = 1-0 transitions of 12 CO, 13 CO, C 18 O and C 17 O.We present a series of velocity-integrated maps, spectra and position-velocity plots that illustrate Galactic arm structures and trace masses on the order of ∼10 6 M per square degree; and include a preliminary catalogue of C 18 O clumps located between l = 330-340 • . Together with information about the noise statistics of the survey these data can be retrieved from the Mopra CO website, the PASA data store and the Harvard Dataverse.