Prospects for Genome Editing of Potato

Dev, Sona S.; Joseph, Jose; D’Rosario, Ligi Lambert

doi:10.5772/intechopen.99278

Cited by 2 publications

(2 citation statements)

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“…The simplicity of CRISPR/Cas9 system made it the most widely applied technology for potato genome editing, as well as for the rest of plant species (Gao, 2021). Since the first report on a CRISPR/Cas9-mediated genome editing in potato in 2015 (Wang et al, 2015), this system has been applied in number of basic research studies and in improving important traits in potato, including its nutritional quality, modification of tuber starch (Hameed et al, 2018;Nadakuduti et al, 2018;Dev et al, 2021) and are summarized in Table 2, together with more recently published reports.…”

Section: Genome Editing In Potatomentioning

confidence: 99%

“…There are many reports concerning successfully gene transfer of economically important genes in potato and GE varieties have been developed for a wide range of traits (Pribylova et al, 2006;Halterman et al, 2016;Zaheer and Akhtar, 2016;Hameed et al, 2018;Nadakuduti et al, 2018;Dev et al, 2021;Rakosy-Tican and Molnar, 2021). However, the use of biotechnology for potato improvement has been ultimately constrained by the regulation process and the public perception (Ghislain and Douches, 2020).…”

Section: Future Perspectives and Concluding Remarksmentioning

confidence: 99%

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State of the Art of Genetic Engineering in Potato: From the First Report to Its Future Potential

et al. 2022

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Potato (Solanum tuberosum L.) is a crop of world importance that produces tubers of high nutritional quality. It is considered one of the promising crops to overcome the challenges of poverty and hunger worldwide. However, it is exposed to different biotic and abiotic stresses that can cause significant losses in production. Thus, potato is a candidate of special relevance for improvements through conventional breeding and biotechnology. Since conventional breeding is time-consuming and challenging, genetic engineering provides the opportunity to introduce/switch-off genes of interest without altering the allelic combination that characterize successful commercial cultivars or to induce targeted sequence modifications by New Breeding Techniques. There is a variety of methods for potato improvement via genetic transformation. Most of them incorporate genes of interest into the nuclear genome; nevertheless, the development of plastid transformation protocols broadened the available approaches for potato breeding. Although all methods have their advantages and disadvantages, Agrobacterium-mediated transformation is the most used approach. Alternative methods such as particle bombardment, protoplast transfection with polyethylene glycol and microinjection are also effective. Independently of the DNA delivery approach, critical steps for a successful transformation are a rapid and efficient regeneration protocol and a selection system. Several critical factors affect the transformation efficiency: vector type, insert size, Agrobacterium strain, explant type, composition of the subculture media, selective agent, among others. Moreover, transient or stable transformation, constitutive or inducible promoters, antibiotic/herbicide resistance or marker-free strategies can be considered. Although great efforts have been made to optimize all the parameters, potato transformation protocols are highly genotype-dependent. Genome editing technologies provide promising tools in genetic engineering allowing precise modification of targeted sequences. Interestingly, transient expression of genome editing components in potato protoplasts was reported to generate edited plants without the integration of any foreign DNA, which is a valuable aspect from both a scientific and a regulatory perspective. In this review, current challenges and opportunities concerning potato genetic engineering strategies developed to date are discussed. We describe their critical parameters and constrains, and the potential application of the available tools for functional analyses or biotechnological purposes. Public concerns and safety issues are also addressed.

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Section: Genome Editing In Potatomentioning

confidence: 99%

Section: Future Perspectives and Concluding Remarksmentioning

confidence: 99%

State of the Art of Genetic Engineering in Potato: From the First Report to Its Future Potential

et al. 2022

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Genome Editing Technology for Genetic Amelioration of Fruits and Vegetables for Alleviating Post-Harvest Loss

et al. 2022

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Food security and crop production are challenged worldwide due to overpopulation, changing environmental conditions, crop establishment failure, and various kinds of post-harvest losses. The demand for high-quality foods with improved nutritional quality is also growing day by day. Therefore, production of high-quality produce and reducing post-harvest losses of produce, particularly of perishable fruits and vegetables, are vital. For many decades, attempts have been made to improve the post-harvest quality traits of horticultural crops. Recently, modern genetic tools such as genome editing emerged as a new approach to manage and overcome post-harvest effectively and efficiently. The different genome editing tools including ZFNs, TALENs, and CRISPR/Cas9 system effectively introduce mutations (In Dels) in many horticultural crops to address and resolve the issues associated with post-harvest storage quality. Henceforth, we provide a broad review of genome editing applications in horticulture crops to improve post-harvest stability traits such as shelf life, texture, and resistance to pathogens without compromising nutritional value. Moreover, major roadblocks, challenges, and their possible solutions for employing genome editing tools are also discussed.

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Prospects for Genome Editing of Potato

Cited by 2 publications

References 76 publications

State of the Art of Genetic Engineering in Potato: From the First Report to Its Future Potential

State of the Art of Genetic Engineering in Potato: From the First Report to Its Future Potential

Genome Editing Technology for Genetic Amelioration of Fruits and Vegetables for Alleviating Post-Harvest Loss

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