Prospects for Improving African Trypanosomiasis Chemotherapy

Moore, Anne

doi:10.1086/429935

Cited by 20 publications

(15 citation statements)

References 25 publications

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“…2 Moreover, increasing melarsoprol failure rates have been reported in several sites. [3][4][5][6][7] Efl ornithine (α-difl uoromethylornithine or DFMO) is the only new treatment registered for HAT in the past six decades. A trypanostatic drug, efl ornithine acts by suicide inactivation of ornithine decarboxylase, an enzyme essential for polyamine synthesis that is needed for cell multiplication and diff erentiation.…”

Section: Introductionmentioning

confidence: 99%

Nifurtimox-eflornithine combination therapy for second-stage African Trypanosoma brucei gambiense trypanosomiasis: a multicentre, randomised, phase III, non-inferiority trial

Priotto¹,

Kasparian²,

Mutombo³

et al. 2009

The Lancet

469

387

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Section: Introductionmentioning

confidence: 99%

Nifurtimox-eflornithine combination therapy for second-stage African Trypanosoma brucei gambiense trypanosomiasis: a multicentre, randomised, phase III, non-inferiority trial

Priotto¹,

Kasparian²,

Mutombo³

et al. 2009

The Lancet

469

387

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“…Trypanosomiasis is fatal if left untreated and chemotherapy which forms the most important and major aspect of control and eradication of the disease in African countries is beset with problems of toxicity and increasing incidence of resistance among the trypanosomes to the existing drugs (Kioy & Mattock, 2005;Moore, 2005). The search for new drugs and formulations that are safe, affordable and effective against both early and late stages of the disease is recommended (Jannin and Cattand, 2004;Chibale, 2005;Pink et al, 2005).…”

Section: Introductionmentioning

confidence: 99%

Some biochemical and haematological effects of black seed (Nigella sativa) oil on T. brucei-infected rats

Ekanem¹,

Yusuf²

2010

African Journal of Biomedical Research

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The effect of black seed oil (Nigella sativa oil) on parasitaemia, some serum and liver enzymes as well as some haematological parameters in Trypanosoma brucei-infected rats was investigated. The results show there was low parasitaemia and extension of life span of rats from 12 days of the infected untreated (control) rats to 22 days for the infected black seed oil-treated rats. Results also show significant increases in activities of serum alkaline phosphatase as well as glutamate oxaloacetate and glutamate pyruvate transaminases with decreases in the liver enzyme activities. Protein concentrations show significant decreases in the serum and increases in the liver. There were also significant increases in the haemoglobin (Hb) concentration, packed cell volume (PCV), red blood cell (RBC), white blood cell (WBC) and platelet counts of infected oil-treated rats when compared with the infected untreated. We suggest that black seed oil has trypanocidal properties and probably stimulate the host immune system to control parasite proliferation thereby making it a possible agent for managing African sleeping sickness despite possible gradual damage to host organs as shown by increases in some serum enzymes.

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“…2 The combination of drugs used to treat HAT are generally either toxic or have difficult administration regimens attached to them. The second stage of HAT (resulting in neurologic symptoms) is only treatable with the drugs melarsoprol and elfornithine.…”

Section: Introductionmentioning

confidence: 99%

Development of an Alamar Blue™ Viability Assay in 384-Well Format for High Throughput Whole Cell Screening of Trypanosoma brucei brucei Bloodstream Form Strain 427

Sykes¹,

Avery²

2009

The American Journal of Tropical Medicine and Hygiene

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Abstract. There is an urgent need for new compounds for the drug development pipeline for treatment of patients with African sleeping sickness. One approach for identifying such compounds is by high throughput screening (HTS) of compound collections. For time and cost considerations, there is a need for the development of an assay that uses at least 384-well formats. To our knowledge, there are currently no viability assays for whole cell screening of trypanosomes in the 384-well plate format. We have developed and optimized an Alamar Blue viability assay in a 384-well format for Trypanosoma brucei brucei bloodstream form strain 427 (BS427). The assay had a Z′ > 0.5 and tolerated a final dimethylsulfoxide concentration of 0.42%. Drug sensitivity was compared with those reported from previously developed 96-well methods and was found to be comparable. The sensitivity and cost benefit of the Alamar Blue assay make it an excellent candidate for HTS application.

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Prospects for Improving African Trypanosomiasis Chemotherapy

Cited by 20 publications

References 25 publications

Nifurtimox-eflornithine combination therapy for second-stage African Trypanosoma brucei gambiense trypanosomiasis: a multicentre, randomised, phase III, non-inferiority trial

Nifurtimox-eflornithine combination therapy for second-stage African Trypanosoma brucei gambiense trypanosomiasis: a multicentre, randomised, phase III, non-inferiority trial

Some biochemical and haematological effects of black seed (Nigella sativa) oil on T. brucei-infected rats

Development of an Alamar Blue™ Viability Assay in 384-Well Format for High Throughput Whole Cell Screening of Trypanosoma brucei brucei Bloodstream Form Strain 427

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