Prospects of Biotechnology in Horticultural Breeding

Marrewijk, G.A.M. van

doi:10.17660/actahortic.1994.369.17

Cited by 2 publications

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“…Thus, the search for an alternative breeding method is imperative. Genetic transformation offers an alternative approach for introducing desirable traits, such as resistance to herbicides, diseases, and insects; or developing new floral colors (van Marrewijk, 1994).…”

Section: Suspension Cultures Cell Suspension Cultures Of Iris Germanicamentioning

confidence: 99%

Genetic Transformation of Iris germanica Mediated by Agrobacterium tumefaciens

Jeknić¹,

Lee²,

Davis³

et al. 1999

jashs

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A protocol was developed for production of transgenic iris plants (Iris germanica L. `Skating Party') from regenerable suspension cultures via Agrobacterium-mediated transformation. We tested a series of selection agents, and identified hygromycin and geneticin as the most suitable for selecting transformed iris cells. Suspension cultures of iris were cocultured for 3 days with A. tumefaciens LBA 4404(pTOK233) carrying an intron-interrupted uidA (GUS) gene encoding β-glucuronidase, and hpt (hygromycin) and nptII (geneticin) selectable marker genes. Hygromycin- or geneticin-resistant calli having GUS enzyme activity were identified and used to induce plant regeneration. More than 300 morphologically normal transgenic iris plants were obtained in ≈6 months. About 80% of the transformants were GUS-positive and NPTII-positive (paromomycin-resistant). Integration of transgenes into the nuclear genome of iris plants was confirmed by Southern blot analysis. We have, therefore, developed an efficient A. tumefaciens-mediated transformation system for Iris germanica, which will allow future improvement of this horticulturally important ornamental monocot via genetic engineering.

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Section: Suspension Cultures Cell Suspension Cultures Of Iris Germanicamentioning

confidence: 99%

Genetic Transformation of Iris germanica Mediated by Agrobacterium tumefaciens

Jeknić¹,

Lee²,

Davis³

et al. 1999

jashs

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show abstract

“…In addition to conventional breeding, genetic transformation offers an alternative approach to improving traits such as resistance to herbicides, diseases, and insects, or obtaining new floral colors (van Marrewijk, 1994). Genetic transformation of iris has not been reported, probably due in part to the lack of an efficient plant regeneration system.…”

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confidence: 99%

Improved Plant Regeneration from Suspension-cultured Cells of Iris germanica L. `Skating Party'

Wang¹,

Jeknić²,

Ernst³

et al. 1999

HortSci

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To improve the efficiency of iris plant regeneration, we tested the influence of several combinations of Kin and NAA in culture media on the induction of morphogenesis and the subsequent development of plantlets. The highest rates of regeneration (67%) were consistently observed in induction media containing 0.5 μm NAA and either 2.5 or 12.5 μm Kin. Developing medium containing 1.25 μm BA was optimal for high regeneration rates and a high percentage of plantlets simultaneously developing shoots and roots. Rooted plantlets were easily acclimatized and transplanted to various soil mixtures, then grown in the greenhouse. Under optimal conditions as many as 8000 plantlets could be regenerated from 1 g of cells in ≈4 months. Chemical names used: kinetin (Kin); 1-naphthaleneacetic acid (NAA); N6-benzyladenine (BA).

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Prospects of Biotechnology in Horticultural Breeding

Cited by 2 publications

References 0 publications

Genetic Transformation of Iris germanica Mediated by Agrobacterium tumefaciens

Genetic Transformation of Iris germanica Mediated by Agrobacterium tumefaciens

Improved Plant Regeneration from Suspension-cultured Cells of Iris germanica L. `Skating Party'

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