Prospects of chitosan-based scaffolds for growth factor release in tissue engineering

Sivashankari, P.; Prabaharan, M.

doi:10.1016/j.ijbiomac.2016.02.043

Cited by 107 publications

(46 citation statements)

References 71 publications

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“…[14] Moreover, CMC has other known beneficial properties such as high moisture retention, low inflammatory, toxicity and antimicrobial responses, and promotion of cell adhesion, migration and proliferation. [27,28] In summary, the ability to 3D print human iPSCs to then expand and generate cells of different lineages provides an unprecedented opportunity to form different, authentic, and renewable body tissues. To this end, the present body of work represents a first important step, with further refinement of method expected to enhance tissue identity, architecture and function to better model development and diseases, for pharmaceuticals screening and assessing in vivo function and safety in animal models toward transplantation therapies.…”

Section: Resultsmentioning

confidence: 99%

3D Bioprinting Human Induced Pluripotent Stem Cell Constructs for In Situ Cell Proliferation and Successive Multilineage Differentiation

Tomaskovic-Crook

Wallace

et al. 2017

Adv Healthcare Materials

188

164

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The ability to create 3D tissues from induced pluripotent stem cells (iPSCs) is poised to revolutionize stem cell research and regenerative medicine, including individualized, patient‐specific stem cell‐based treatments. There are, however, few examples of tissue engineering using iPSCs. Their culture and differentiation is predominantly planar for monolayer cell support or induction of self‐organizing embryoids (EBs) and organoids. Bioprinting iPSCs with advanced biomaterials promises to augment efforts to develop 3D tissues, ideally comprising direct‐write printing of cells for encapsulation, proliferation, and differentiation. Here, such a method, employing a clinically amenable polysaccharide‐based bioink, is described as the first example of bioprinting human iPSCs for in situ expansion and sequential differentiation. Specifically, we have extrusion printed the bioink including iPSCs, alginate (Al; 5% weight/volume [w/v]), carboxymethyl‐chitosan (5% w/v), and agarose (Ag; 1.5% w/v), crosslinked the bioink in calcium chloride for a stable and porous construct, proliferated the iPSCs within the construct and differentiated the same iPSCs into either EBs comprising cells of three germ lineages—endoderm, ectoderm, and mesoderm, or more homogeneous neural tissues containing functional migrating neurons and neuroglia. This defined, scalable, and versatile platform is envisaged being useful in iPSC research and translation for pharmaceuticals development and regenerative medicine.

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Section: Resultsmentioning

confidence: 99%

3D Bioprinting Human Induced Pluripotent Stem Cell Constructs for In Situ Cell Proliferation and Successive Multilineage Differentiation

Tomaskovic-Crook

Wallace

et al. 2017

Adv Healthcare Materials

188

164

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“…The absorption efficiency of IGF-1 and BMP-2 was found to be 90% ± 2% and 87% ± 2%, respectively. In vivo studies revealed that chitosan scaffolds loaded with IGF-1 showed significant osteoblastic differentiation than BMP-2-loaded chitosan scaffolds [17]. …”

Section: Fabrication Of Natural Biopolymersmentioning

confidence: 99%

Fabrication of Porous Materials from Natural/Synthetic Biopolymers and Their Composites

et al. 2016

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Biopolymers and their applications have been widely studied in recent years. Replacing the oil based polymer materials with biopolymers in a sustainable manner might give not only a competitive advantage but, in addition, they possess unique properties which cannot be emulated by conventional polymers. This review covers the fabrication of porous materials from natural biopolymers (cellulose, chitosan, collagen), synthetic biopolymers (poly(lactic acid), poly(lactic-co-glycolic acid)) and their composite materials. Properties of biopolymers strongly depend on the polymer structure and are of great importance when fabricating the polymer into intended applications. Biopolymers find a large spectrum of application in the medical field. Other fields such as packaging, technical, environmental, agricultural and food are also gaining importance. The introduction of porosity into a biomaterial broadens the scope of applications. There are many techniques used to fabricate porous polymers. Fabrication methods, including the basic and conventional techniques to the more recent ones, are reviewed. Advantages and limitations of each method are discussed in detail. Special emphasis is placed on the pore characteristics of biomaterials used for various applications. This review can aid in furthering our understanding of the fabrication methods and about controlling the porosity and microarchitecture of porous biopolymer materials.

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“…[58] Alginate exhibits excellent biocompatibility and biodegradability, as well as an ability to be readily processed for 3D scaffolding materials. [59] Chitosan is one of the few natural polymers with a polycationic nature, providing good adhesive properties and high biocompatibility, [60] while silk fibroin presents low immunogenicity, high mechanical strength, and controllable degradation behavior. [61] However, unlike ECM-derived molecules these do not provide biological interactive motifs, requiring combination with other materials or immobilization of specific ligands to effectively control cellular responses.…”

Section: Conventional Controlled Release Systemsmentioning

confidence: 99%

Biomaterials for Sequestration of Growth Factors and Modulation of Cell Behavior

Teixeira

Domingues

Shevchuk

et al. 2020

Adv Funct Materials

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Growth factors (GFs) are proteins secreted by cells that regulate a variety of biological processes. Although they have long been proposed as potent therapeutic agents, their administration in a soluble form has proven costly and ineffective due to their short half-lives in biological environments. Biomaterial-based approaches are increasingly sought as alternatives to improve the efficacy or, ideally, replace the need for exogenous administration of GFs in regenerative medicine strategies. The means by which these systems evolve from biomaterials for conventional controlled release of GFs to the recent extracellular matrix (ECM)-inspired approaches for sequestering these labile molecules and regulating their spatiotemporal activity and presentation are reviewed. Focus is placed on biomaterials functionalized either with ECM components, which show promiscuous GF binding, or with targeted GF ligands (antibodies, aptamers, or peptides). The potential of synthetic platforms with abiotic affinity as cost-effective alternatives to the current biological ligands is also discussed. Overall, the various GF sequestering systems developed so far have remarkably improved the activity of GFs at reduced doses and, in some cases, completely avoided the need for their exogenous administration to guide cell fates. These bioinspired concepts thus enable the rational exploration of the full therapeutic potential of GFs in regenerative medicine.

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Prospects of chitosan-based scaffolds for growth factor release in tissue engineering

Cited by 107 publications

References 71 publications

3D Bioprinting Human Induced Pluripotent Stem Cell Constructs for In Situ Cell Proliferation and Successive Multilineage Differentiation

3D Bioprinting Human Induced Pluripotent Stem Cell Constructs for In Situ Cell Proliferation and Successive Multilineage Differentiation

Fabrication of Porous Materials from Natural/Synthetic Biopolymers and Their Composites

Biomaterials for Sequestration of Growth Factors and Modulation of Cell Behavior

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