Prospects of Improving Nitrogen Use Efficiency in Potato: Lessons From Transgenics to Genome Editing Strategies in Plants

Tiwari, Jagesh Kumar; Buckseth, Tanuja; Singh, Rajesh Kumar; Kumar, Manoj; Kant, Surya

doi:10.3389/fpls.2020.597481

Cited by 29 publications

(11 citation statements)

References 55 publications

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“…To enhance NUE, it’s essential to grasp the plant response to N treatments, particularly to N limitation at each the physiological and transcriptomic levels. During this study, the candidate genes selected were supported by the previous studies, and can have a potential role in rising NUE [ 1 , 3 , 44 ].…”

Section: Discussionmentioning

confidence: 99%

“…The NRTs , auxin -induced, auxin -regulated, NRT 1.1, chloroplast pigment-binding, chlorophyll a-b binding, ATP synthase, wall-associated kinase, and NADPH genes kept consistent between RNA-Seq and qPCR detection. The candidate genes could be used for genetic manipulation for increasing NUE in potatoes via transgenic or CRISPR/Cas9 or base-editing technologies [ 44 ]. Exploring the molecular functions of these genes requires further experimental verification.…”

Section: Discussionmentioning

confidence: 99%

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Transcriptome analysis reveals multiple effects of nitrogen accumulation and metabolism in the roots, shoots, and leaves of potato (Solanum tuberosum L.)

Guo

Jia

et al. 2022

BMC Plant Biol

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Background Nitrogen (N) is a major element and fundamental constituent of grain yield. N fertilizer plays an essential role in the roots, shoots, and leaves of crop plants. Here, we obtained two N-sensitive potato cultivars. Results The plants were cultivated in the pots using N-deficient and N-sufficient conditions. Crop height, leaf chlorophyll content, dry matter, and N-accumulation significantly decreased under N-deficient conditions. Furthermore, we performed a comprehensive analysis of the phenotype and transcriptome, GO terms, and KEGG pathways. We used WGCNA of co-expressed genes, and 116 differentially expressed hub genes involved in photosynthesis, nitrogen metabolism, and secondary metabolites to generate 23 modules. Among those modules, six NRT gene families, four pigment genes, two auxin-related genes, and two energy-related genes were selected for qRT-PCR validation. Conclusions Overall, our study demonstrates the co-expressed genes and potential pathways associated with N transport and accumulation in potato cultivars’ roots, shoots, and leaves under N-deficient conditions. Therefore, this study provides new ideas to conduct further research on improving nitrogen use efficiency in potatoes.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Transcriptome analysis reveals multiple effects of nitrogen accumulation and metabolism in the roots, shoots, and leaves of potato (Solanum tuberosum L.)

Guo

Jia

et al. 2022

BMC Plant Biol

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“…Considerable research work on abiotic stress has been reported in cereals and other crops, but not in potato. Recently, we have proposed the use of CRISPR/Cas to manipulate N metabolism genes for improving nitrogen use efficiency in potato (Tiwari et al, 2020).…”

Section: Application Of Crispr/cas In Potato Biotic and Abiotic Stres...mentioning

confidence: 99%

CRISPR/Cas Genome Editing in Potato: Current Status and Future Perspectives

et al. 2022

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“…Researchers have engineered crops for enhanced NUE, which improved the transport of nutrients, nutrient use, growth, and productivity. 63 For instance, the rice nitrate transporter gene NRT1.1B was edited via the CRISPR-based cytosine base editing system (CBE), and the resulting mutant showed improved NUE compared with wild-type rice. 53 The CBE generated a C → T substitution in NRT1.1B and changed the Thr amino acid present at position 327 into Met.…”

Section: Can Crispr Technology Achieve the End Hunger And Resolve The...mentioning

confidence: 99%

CRISPR-Based Crop Improvements: A Way Forward to Achieve Zero Hunger

Ahmad

Tang

Shahzad

et al. 2021

J. Agric. Food Chem.

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Zero hunger is one of the sustainable development goals set by the United Nations in 2015 to achieve global food security by 2030. The current harvest of crops is insufficient; feeding the world’s population and meeting the goal of zero hunger by 2030 will require larger and more consistent crop production. Clustered regularly interspaced short palindromic repeats-associated protein (CRISPR-Cas) technology is widely used for the plant genome editing. In this review, we consider this technology as a potential tool for achieving zero hunger. We provide a comprehensive overview of CRISPR-Cas technology and its most important applications for food crops’ improvement. We also conferred current and potential technological breakthroughs that will help in breeding future crops to end global hunger. The regulatory aspects of deploying this technology in commercial sectors, bioethics, and the production of transgene-free plants are also discussed. We hope that the CRISPR-Cas system will accelerate the breeding of improved crop cultivars compared with conventional breeding and pave the way toward the zero hunger goal.

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Prospects of Improving Nitrogen Use Efficiency in Potato: Lessons From Transgenics to Genome Editing Strategies in Plants

Cited by 29 publications

References 55 publications

Transcriptome analysis reveals multiple effects of nitrogen accumulation and metabolism in the roots, shoots, and leaves of potato (Solanum tuberosum L.)

Transcriptome analysis reveals multiple effects of nitrogen accumulation and metabolism in the roots, shoots, and leaves of potato (Solanum tuberosum L.)

CRISPR/Cas Genome Editing in Potato: Current Status and Future Perspectives

CRISPR-Based Crop Improvements: A Way Forward to Achieve Zero Hunger

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