Prospects of Laser Doppler flowmetry application in assessment of skin microcirculation in diabetes

Куликов, Д. А.; Глазков, А. А.; Kovaleva, Yu. A.; Balashova, N. V.; Куликов, А. В.

doi:10.14341/dm8014

Cited by 24 publications

(3 citation statements)

References 51 publications

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“…Максимум информации о состоянии сосудов микроциркуляторного русла и капиллярного кровотока представляется возможным получить с использованием неинвазивного метода лазерной допплеровской флоуметрии (ЛДФ) [17,18]. Существует множество вариантов применения данного метода в медицинских исследованиях и практике.…”

Section: Introductionunclassified

Examination of the Microvasculature During Wound Process of the Oral Mucosa when Using Dual-Wavelength Photodynamic Therapy: a Pre-Clinical Experimental Randomized Study

Durnovo,

Tarakanova,

Shakhova

et al. 2023

Kuban. nauсh. med. vestnik

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Background. Healing of open wounds in the oral cavity is often accompanied by infection and constant traumatization, which lead to impaired blood supply to the tissues, lack of trophic support and low oxygen supply to increasing proliferative processes. Recently, photodynamic therapy has taken its place among the methods for stimulating regeneration of oral mucosal tissues. The authors consider it essential to study the influence of photodynamic treatment on the functional state of the microvasculature in the wound tissues, which determines the transcapillary exchange and the tissue respiration, providing the tissue regeneration.Objective. To study the photodynamic effect of sequential dual-wavelength radiation on the microvasculature in the wound tissues of the oral mucosa in vivo.Methods. The study was carried out on 36 female outbred Wistar rats, weighing 200–250 g. The animals were divided into two groups: control group (n = 18) and experimental group (n = 18). Wound surfaces healed by secondary intention were modeled on the cheek mucosa of the animals. Dual-wavelength photodynamic therapy was performed in the 1st postoperative day in experimental group. The microcirculatory function was assessed by Doppler flowmetry before, and on days 3, 7 and 14 after wounding. The results were processed using IBM SPSS Statistics 26.0.0.1 (IBM Inc., USA).Results. It has been established that dual-wavelength photodynamic therapy has a positive effect on the restoration of the microvasculature of the wound tissues. This therapy facilitated bypassing for microcirculatory flow on the 3rd day of the study, and increased the perfusion of tissues on the 7th. The experimental group did not have congestive-ischaemic phenomena in the wound region; the microcirculatory flow was augmented through the microcirculation regulation by the 14th day.Conclusion. Following the obtained results the use of dual-wavelength photodynamic therapy can be considered as a promising method for restoration of the microvasculature of tissues in the oral mucosal trauma.

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Section: Introductionunclassified

Examination of the Microvasculature During Wound Process of the Oral Mucosa when Using Dual-Wavelength Photodynamic Therapy: a Pre-Clinical Experimental Randomized Study

Durnovo,

Tarakanova,

Shakhova

et al. 2023

Kuban. nauсh. med. vestnik

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“…Currently, laser Doppler flowmetry is among the best modern non-invasive techniques that allow assessing the state of the microvasculature [5]. The novelty of the methodological approach implemented in the course of our study was the possibility of using laser Doppler flowmetry parameters for assessing the dynamics of reparative regeneration in skin wounds.…”

Section: Introductionmentioning

confidence: 99%

Features of microcirculatory responses in experimental wound area in white rats

Loiko,

Savkina,

Stepanova

et al. 2022

Saratov Med J

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Objective: to examine changes in the microcirculatory bed parameters via laser Doppler flowmetry in the course of wound healing and the possibility of their use for upgrading the technology of evaluating the effectiveness of wound healing agents. Materials and Methods. The studies were performed on 25 white rats distributed between two groups: 10 control animals (intact rats) and 15 animals with a full-thickness experimental skin defect. The microcirculation parameters in the skin of experimental wound edges in rats were evaluated using laser Doppler flowmetry, and histological preparations of tissues in the wound area were analyzed. Results. Changes in skin microcirculation at the wound edges were characterized by inflammatory hyperemia manifested by an increase in the perfusion index by 27% and augmented normalized amplitudes of myogenic, respiratory and cardiogenic oscillations. Changes in microcirculation were verified by the morphological picture of inflammation, which reflects an increase in the number of vessels fully filled with arterial and venous blood, as well as in leukocyte infiltration of the wound edges and bottom. Conclusion. Monitoring of microcirculatory disorders occurring in the area of skin wounds allows assessing the dynamics of the reparative process, which could be used for developing and evaluating the effectiveness of existing medicamentous and non-medicamentous methods of stimulating regeneration.

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“…Однако, такая оценка проводится далеко не во всех исследованиях. Достаточно часто авторы ограничиваются лишь анализом статистической значимости различий между группами [5][6][7]. Это не позволяет делать вывод о применимости описываемых методик в диагностических целях.…”

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Assessing Diagnostic Accuracy of Quantitative Data in Biomedical Studies Using Descriptive Statistics and Standardized Mean Difference

Глазков¹,

Kulikov²,

Глазкова³

2020

Math.Biol.Bioinf.

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ROC analysis is the most used method for analyzing the diagnostic accuracy of quantitative data in biomedical research. ROC analysis generates a curve describing the frequencies of true positive and false positive results for different degrees of the analyzed variable. However, in many publications devoted to the application of quantitative diagnostic methods, this analysis is not carried out: researchers report only analysis of statistical significance for the groups difference. In meta-analyses, the estimated parameter is the effect size expressed through standardized mean difference. The article describes the approach, which allows performing ROC analysis using cumulative normal distribution functions for studied and controlling groups. The proposed approach can be used to evaluate the diagnostic accuracy of quantitative variables on the base of one of the sets of descriptive statistics (mean and standard deviation, or median and quartiles) or the value of standardized mean difference. Examples of application of the proposed approach on model data, on data from literature sources, as well as on the authors' own observations are given as an example of assessment of diagnostic accuracy of quantitative variables analyzed in the microcirculation studies in various diseases. The results presented in the article can be used by medical and biological specialists to assess the diagnostic accuracy of various quantitative variables without access to primary data.

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Prospects of Laser Doppler flowmetry application in assessment of skin microcirculation in diabetes

Cited by 24 publications

References 51 publications

Examination of the Microvasculature During Wound Process of the Oral Mucosa when Using Dual-Wavelength Photodynamic Therapy: a Pre-Clinical Experimental Randomized Study

Examination of the Microvasculature During Wound Process of the Oral Mucosa when Using Dual-Wavelength Photodynamic Therapy: a Pre-Clinical Experimental Randomized Study

Features of microcirculatory responses in experimental wound area in white rats

Assessing Diagnostic Accuracy of Quantitative Data in Biomedical Studies Using Descriptive Statistics and Standardized Mean Difference

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