Prospects of Red King Crab Hepatopancreas Processing: Fundamental and Applied Biochemistry

Ponomareva, Tatyana; Timchenko, Maria; Filippov, Michael; Lapaev, Sergey; Sogorin, Evgeny

doi:10.3390/recycling6010003

Cited by 13 publications

(7 citation statements)

References 36 publications

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“…The rate of HA cleavage is an order of magnitude higher than the rate of hydrolysis by a commercial analog (Lydazum) when normalized to the total protein in the studied preparations of hyaluronidase. Since red king crab catch amounts to large volumes in different countries generating hepatopancreas as a waste product of crab processing it is safe to assume that it generates enough of a raw material for production of a new potential medical drug hyaluronidase on industrial scale 20 .…”

Section: Introductionmentioning

confidence: 99%

β-elimination of hyaluronate by red king crab hyaluronidase

Sliadovskii¹,

Ponomareva²,

Молчанов

et al. 2021

Sci Rep

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Crustacean hyaluronidases are poorly understood both in terms of their enzymatic properties and in terms of their structural features. In this work, we show that the hepatopancreas homogenate of the red king crab has a hyaluronidase activity that is an order of magnitude higher than its commercial counterpart. Zymography revealed that the molecular weight of a protein with hyalorunidase activity is 40–50 kDa. Analysis of the hepatopancreas transcriptome and results of cloning and sequencing of cDNA revealed a hyaluronidase sequence with an expected molecular weight of 42.5 kDa. Further analysis showed that hyaluronat enzymatic cleavage follows the $$\beta $$ β -elimination mechanism, which is well known for bacterial hyaluronidases. The results of ion-exchange chromatography showed that the final product of hyaluronate degradation is unsaturated tetrasaccharide. Thus, we identified a new hyaluronidase of higher eukaryotes, which is not integrated into the modern classification of hyaluronidases.

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Section: Introductionmentioning

confidence: 99%

β-elimination of hyaluronate by red king crab hyaluronidase

Sliadovskii¹,

Ponomareva²,

Молчанов

et al. 2021

Sci Rep

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“…Our research has revealed that the muscles are a structural tissue characterized by a high protein content (19.41%) and low fat content (0.87%), which aligns with previous findings reported for this species by Chen et al [ 17 ] and Wang et al [ 19 ]. The hepatopancreas, a multifunctional organ responsible for lipid and cholesterol absorption and storage [ 35 , 36 ], exhibits a high lipid content ranging from 15.01% to 45.09% in Chinese mitten crabs [ 19 , 24 ], while the protein content is relatively low, ranging from 6.29% to 10.28% [ 19 , 31 ]. In the examined crabs from the Odra River estuary, the fat content in the hepatopancreas was lower (11.67%), while the protein content was higher (13.62%).…”

Section: Discussionmentioning

confidence: 99%

Nutritional Quality and Fatty Acids Composition of Invasive Chinese Mitten Crab from Odra Estuary (Baltic Basin)

Czerniejewski,

Bienkiewicz,

Tokarczyk

2023

Foods

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The Chinese mitten crab (Eriocheir sinensis) is recognized as an invasive species in Europe but increasing fishing efforts may hold economic benefits and yield positive ecological and nutritional outcomes. The objective of this study was to determine the yield of edible parts and the compositional and nutritional characteristics of this crab, especially as a source of n-3 PUFA. The overall yield of edible parts amounted to 38.09%, with males (27.72%) exhibiting a higher meat content compared to females (25.30%). The gonads displayed the highest protein content (24.12%), while the hepatopancreas (11.67%) showcased the highest fat content. Furthermore, the fatty acid composition varied depending on the distribution within different crab segments and gender and individual size. Notably, the gonad lipids contained the most nutritionally valuable n-3 fatty acids, followed by muscle and hepatopancreas lipids. The determined index of atherogenicity (IA) from 0.2 for gonadal lipids to 0.42 for hepatopancreas lipids, index of thrombogenicity (IT) in the range of 0.10 for gonads to 0.41 for hepatopancreas, and flesh lipid quality (FLQ) from 6.9 for hepatopancreas to 23 for muscle lipids indicate their pro-health properties. The ratio of n-3 to n-6 fatty acids showed Chinese mitten crab as an excellent source of oil that can be used for food fortification and dietary supplement production.

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“…It is considered a delicacy that is valued by food markets and has a stable demand supporting high prices. Moreover, by-products of P. camtschaticus such as hepatopancreas, hemolymph, and shells are also considered important sources for chitin, chitosan, proteolytic enzymes, fatty acids, and other substances [16][17][18].…”

Section: Introductionmentioning

confidence: 99%

Prey Selectivity in Juvenile Red King Crabs from the Coastal Barents Sea

Павлова

Dvoretsky

2022

Diversity

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The invasive red king crab, Paralithodes camtschaticus, has become an abundant and important component in the food web of the coastal Barents Sea and can affect the structure and functioning of the local benthic communities through competition and predation. Although dietary composition and feeding behavior of the crab have been intensively studied, prey selectivity in this species under natural conditions remains poorly defined. For this reason, juvenile red king crabs and benthic samples were collected simultaneously at five coastal sites in Kola Bay to reveal the species composition and structure of feeding habits and the diet of red king crabs. The results of stomach and gut content analyses coupled with calculated Ivlev’s indices indicated that 2–5-year-old crabs frequently consumed bivalve mollusks in relative proportions to prey field biomasses. At all sites, juveniles selectively rejected polychaetes. In soft-bottom communities, when the average density of Bivalvia decreased, the crabs showed increased preference for Gastropoda, Crustacea, and Echinodermata. As a result of selective feeding focused on infaunal suspension-feeding mollusks, juvenile red king crabs have altered the structure of benthic communities in the mouth of Kola Bay. Our results may have important implications for evaluating the consequences of the crab introduction and its population management.

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Prospects of Red King Crab Hepatopancreas Processing: Fundamental and Applied Biochemistry

Cited by 13 publications

References 36 publications

β-elimination of hyaluronate by red king crab hyaluronidase

β-elimination of hyaluronate by red king crab hyaluronidase

Nutritional Quality and Fatty Acids Composition of Invasive Chinese Mitten Crab from Odra Estuary (Baltic Basin)

Prey Selectivity in Juvenile Red King Crabs from the Coastal Barents Sea

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