Prospects of ultraviolet resonance Raman spectroscopy in supramolecular chemistry on proteins

Kumar, Vikas; Holtum, Tim; Voskuhl, Jens; Giese, Michael; Schräder, Thomas; Schlücker, Sebastian

doi:10.1016/j.saa.2021.119622

Cited by 8 publications

(9 citation statements)

References 29 publications

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“…Examples from supramolecular chemistry are artificial carboxylate binders such as guanidiniocarbonylpyrrole (GCP)-based ligands, [5][6][7][8][9] which exhibit electronic resonances around 280 to 310 nm range. [10] In addition to the neat artificial ligand itself, the monitoring of molecular recognition by target peptides and proteins containing aromatic amino acids is prevented by the UV-autofluorescence of the latter. Therefore, there is a need for an approach to suppress UV-autofluorescence when Raman excitation wavelengths around 280 nm are employed.…”

Section: Introductionmentioning

confidence: 99%

Picosecond UV Kerr‐gated Raman spectroscopy with 280 nm laser excitation

Holtum

Reichenauer

Kumar

et al. 2023

J Raman Spectroscopy

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Raman spectra obtained by UV laser excitation above 260 nm are contaminated by spectrally overlapping UV‐excited fluorescence. Here, we demonstrate a picosecond UV Kerr‐gated Raman spectroscopy setup to suppress the fluorescence in Raman spectra excited by 280 nm laser pulses. Specifically, we use a model sample that consists of acetonitrile contaminated by a small amount of toluene for generating UV‐excited fluorescence. We were able to acquire a Raman spectrum of acetonitrile by suppressing the UV‐excited fluorescence of toluene by the action of a 1‐mm thick fused silica‐based UV Kerr gate operated by 1030 nm, ~1 ps gating pulses. These results also indicate the challenges that must be overcome in the future for realizing UV‐fluorescence‐free UV resonance Raman (UVRR) spectroscopy studies of molecules exhibiting electronic resonances around 280 nm using laser excitation wavelengths right at or close to this resonance.

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Section: Introductionmentioning

confidence: 99%

Picosecond UV Kerr‐gated Raman spectroscopy with 280 nm laser excitation

Holtum

Reichenauer

Kumar

et al. 2023

J Raman Spectroscopy

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“…In biological samples, resonant excitation in the ultraviolet (UV) has been shown to be very useful in the Raman spectroscopic characterization of microorganisms [11][12][13] and recently individual eukaryotic cells. 14,15 Based on the rich body of knowledge on the RR spectra of proteins [16][17][18][19] and amino acids, [20][21][22] there is great potential in structural studies of albumins using ultraviolet RR (UVRR) spectroscopy. 23,24 When the deep UV region around 200 nm is used for RR excitation, the selective enhancement of the signals of amide vibrations occurs, enabling the characterization of the protein secondary structure.…”

Section: Introductionmentioning

confidence: 99%

Ultraviolet Resonance Raman Spectra of Serum Albumins

Spedalieri

Plaickner

Speiser³

et al. 2023

Appl Spectrosc

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The ultraviolet resonance Raman (UVRR) spectra of the two proteins bovine serum albumin (BSA) and human serum albumin (HSA) in an aqueous solution are compared with the aim to distinguish between them based on their very similar amino acid composition and structure and to obtain signals from tryptophan that has only very few residues. Comparison of the protein spectra with solutions of tryptophan, tyrosine, and phenylalanine in comparative ratios as in the two proteins shows that at an excitation wavelength of 220 nm, the spectra are dominated by the strong resonant contribution from these three amino acids. While the strong enhancement of two and one single tryptophan residue in BSA and HSA, respectively, results in pronounced bands assigned to fundamental vibrations of tryptophan, its weaker overtones and combination bands do not play a major role in the spectral range above 1800 cm–1. There, the protein spectra clearly reveal the signals of overtones and combination bands of phenylalanine and tyrosine. Assignments of spectral features in the range of Raman shifts from 3800 to 5100 cm–1 to combinations comprising fundamentals and overtones of tyrosine were supported by spectra of amino acid mixtures that contain deuterated tyrosine. The information in the high-frequency region of the UVRR spectra could provide information that is complementary to near-infrared absorption spectroscopy of the proteins.

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“…For example, defense organizations have begun to use UVRR for standoff detection of explosives. 3,4 UVRR has been used to probe the primary, secondary, and tertiary structures of proteins to study the vibrational modes associated with aromatic amino acid side chains, 5,6 the peptide backbone, 7,8 and interactions between side chains. 9,10 Materials science applications have used UVRR to probe the hybridization of carbon in diamonds and amorphous carbon structures.…”

Section: Introductionmentioning

confidence: 99%

Continuously wavelength tunable, continuous wave laser ideal for UV Raman spectroscopy

Roppel,

Asher

2023

J Raman Spectroscopy

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We utilize a novel, high‐power, tunable, continuous wave (CW) deep UV laser to measure resonance Raman spectra of phenolate solutions with high signal‐to‐noise ratios (SNR). In UV resonance Raman (UVRR), increased coupling of the excitation light with a chromophore can transfer molecules into excited states that cause increased heating and photochemistry. Deep UV lasers have traditionally utilized high peak powers to enable efficient single‐pass nonlinear conversion from visible into near infrared light. Nonlinear phenomena such as the formation of transient radical species, Raman saturation, thermal heating, and dielectric breakdown can introduce extraneous light sources that can complicate the interpretation of the Raman spectrum. Dielectric breakdown can increase the baseline, increase noise, and sometimes saturate the detector, preventing Raman detection. Spontaneous Raman scattering intensities should scale linearly with the excitation light intensity. However, this linear behavior does not always occur with pulsed laser excitation. This occurs because stimulated Raman scattering can cause a superlinear intensity response, or transient absorption can cause sublinear intensity responses. CW laser excitation excites samples with electric fields that are much lower than typical pulsed laser excitation. This eliminates the nonlinear responses. The geometry of our new CW laser enables high gain in the harmonic generation cavities that achieve high harmonic generation efficiencies. Average power in the deep UV is >30 mW for wavelengths as short as 206 nm. In the work here, we demonstrate that CW excitation is ideal for resonance Raman measurements in general to reduce spectral complexity.

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Prospects of ultraviolet resonance Raman spectroscopy in supramolecular chemistry on proteins

Cited by 8 publications

References 29 publications

Picosecond UV Kerr‐gated Raman spectroscopy with 280 nm laser excitation

Picosecond UV Kerr‐gated Raman spectroscopy with 280 nm laser excitation

Ultraviolet Resonance Raman Spectra of Serum Albumins

Continuously wavelength tunable, continuous wave laser ideal for UV Raman spectroscopy

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