Prospects of using Metarhizium anisopliae to check the breeding of insect pest, Oryctes rhinoceros L. in coconut leaf vermicomposting sites

Gopal, Madhuban; Gupta, Alka; Thomas, George V.

doi:10.1016/j.biortech.2005.09.005

Cited by 29 publications

(9 citation statements)

References 8 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Coconut water in the intact coconut is free from microbial contamination and is highly nutritious, rich in many amino acids, vitamins and minerals (Nandakumar 1990). Coconut water from the mature coconut is a waste product from processing the nuts, though it has been used for growing microbes and in plant tissue culture (Anandaraj and Sarma 1997;Gopal et al 2006;Survase et al 2007;Unagul et al 2007;Prabhakaran et al 2008). It is an excellent multiplication medium for plant growth promoting rhizobacteria (Anith 2009).…”

Section: Introductionmentioning

confidence: 98%

The growth of tomato seedlings inoculated with co-cultivated Piriformospora indica and Bacillus pumilus

Anith

Sreekumar

2015

Symbiosis

View full text Add to dashboard Cite

A novel co-culture method using the beneficial root endophytic fungus Piriformospora indica and a plant-growth promoting rhizobacterial strain of Bacillus pumilus to promote the growth of tomato seedlings in plug trays, is described. Coconut water, a waste product from the coconut industry, was used as medium for co-cultivation of the two biological agents. During the co-culture with the fungus, the bacterial strain showed a similar growth rate to that in monoculture in fresh autoclaved coconut water. In contrast, co-culture in potato dextrose broth (PDB), a medium routinely used for cultivation of the endophytic fungus, did not support bacterial growth. Inoculation with the co-culture or a mixture of P. indica and B. pumilus promoted tomato seedling growth significantly when compared with individual application of the two biological agents. No difference was observed with respect to the degree of root colonization in tomato seedlings whether it was done with a monoculture or a co-culture with rhizobacterium. The co-culture system developed reduces the cost of inoculum production as it uses coconut water, a cheap and locally available waste product and a single fermentation vessel. Further research is required to know the endophytic nature of the rhizobacterial strain and its possible role in helping the fungus in root colonization.

show abstract

Section: Introductionmentioning

confidence: 98%

The growth of tomato seedlings inoculated with co-cultivated Piriformospora indica and Bacillus pumilus

Anith

Sreekumar

2015

Symbiosis

View full text Add to dashboard Cite

show abstract

“…M. anisopliae invades and infects the body of an insect by direct penetration of the cuticle or breathing apertures, ingestion into the digestive tract, or wounds [19]. The infected insects lose their appetite and exhibit somewhat sluggish behavior.…”

Section: Discussionmentioning

confidence: 99%

Efficacy of Metarhizium anisopliae isolate MAX-2 from Shangri-la, China under desiccation stress

Chen

Yang

et al. 2014

BMC Microbiol

View full text Add to dashboard Cite

BackgroundMetarhizium anisopliae, a soil-borne entomopathogen found worldwide, is an interesting fungus for biological control. However, its efficacy in the fields is significantly affected by environmental conditions, particularly moisture. To overcome the weakness of Metarhizium and determine its isolates with antistress capacity, the efficacies of four M. anisopliae isolates, which were collected from arid regions of Yunnan Province in China during the dry season, were determined at different moisture levels, and the efficacy of the isolate MAX-2 from Shangri-la under desiccation stress was evaluated at low moisture level.ResultsM. anisopliae isolates MAX-2, MAC-6, MAL-1, and MAQ-28 showed gradient descent efficacies against sterile Tenebrio molitor larvae, and gradient descent capacities against desiccation with the decrease in moisture levels. The efficacy of MAX-2 showed no significant differences at 35% moisture level than those of the other isolates. However, significant differences were found at 8% to 30% moisture levels. The efficacies of all isolates decreased with the decrease in moisture levels. MAX-2 was relatively less affected by desiccation stress. Its efficacy was almost unaffected by the decrease at moisture levels > 25%, but slowly decreased at moisture levels < 25%. By contrast, the efficacies of other isolates rapidly decreased with the decrease in moisture levels. MAX-2 caused different infection characteristics on T. molitor larvae under desiccation stress and in wet microhabitat. Local black patches were found on the cuticles of the insects, and the cadavers dried without fungal growth under desiccation stress. However, dark black internodes and fungal growth were found after death of the insects in the wet microhabitat.ConclusionsMAX-2 showed significantly higher efficacy and superior antistress capacity than the other isolates under desiccation stress. The infection of sterile T. molitor larvae at low moisture level constituted a valid laboratory bioassay system in evaluating M. anisopliae efficacy under desiccation stress.

show abstract

“…major dapat dikembangkan pada media air kelapa dan dapat menyebabkan mortalitas larva Oryctes rhinoceros mencapai 100% sedangkan mortalitas imago bervariasi antara 61,7 -76,7% (Sambiran dan Hosang, 2007a,b;Salim dan Hosang, 2013). Aplikasi M. anisopliae di lapangan dapat mengurangi populasi larva O. rhinoceros (Gopal et al, 2006). Dilaporkan juga bahwa cendawan entomopatogen M. anisopliae var.…”

Section: Musuh Alami P Cumingiiunclassified

Serangan Hama Kelapa Promecotheca cumingii Baly

et al. 2016

View full text Add to dashboard Cite

ABSTRAKLedakan populasi hama Promecotheca cumingii Baly (= nucifera Maul.) (Coleoptera : Chrysomelidae) di Sulawesi Utara, pertama kali terjadi pada tahun 2015 di Kecamatan Modayag, Kabupaten Bolaang Mongondow Timur, Sulawesi Utara. Larva dari hama ini merusak daun dengan cara menggerek masuk kedalam lamina daun (leafminers = pengorok daun). Selama hidup larva dan pupa berada dalam liang gerekannya di daun. Serangga dewasa merusak dan meninggalkan bekas gigitan memanjang pada daun. Pada kerusakan berat, tanaman kelapa kelihatan seperti terbakar, sehingga dapat mengurangi produksi kelapa. Penelitian ini bertujuan untuk mempelajari tingkat kerusakan tanaman, populasi hama dan musuh alaminya. Dalam penelitian ini dipilih 3 lokasi di Kecamatan Modayag kemudian masing-masing lokasi dipilih 5 pohon contoh secara acak dalam areal serangan. Dari setiap pohon diambil satu pelepah daun di bagian tengah tajuk, selanjutnya diambil setiap anak daun ke-10 dan dihitung populasi larva, pupa, imago, dan musuh alami. Hasil penelitian menunjukkan populasi larva dan pupa dari hama P. cumingii di Modayag rata 2,2 individu/anak daun atau sekitar 517 individu/pelepah daun kelapa. Kerusakan hama pada kategori serangan berat dan sangat berat dapat mencapai 94,3% dari 140 tanaman contoh yang diamati dengan rata-rata kerusakan antara 70 -85%. Situasi serangan seperti ini, diperkirakan dapat menyebabkan penurunan produksi sampai 95%. Pemanfaatan musuh alami yang menginfeksi hama ini seperti cendawan entomopatogen Beauveria sp. dan Metarhizium sp. mempunyai peluang untuk menekan populasi hama ini dalam jangka panjang dan ramah lingkungan. Konsepsi pengendalian hama terpadu merupakan salah satu alternatif yang tepat dalam pengendalian hama P. cumingii di lapangan, dengan memanfaatkan semua komponen pengendalian yang sesuai, supaya dapat meningkatkan sistem usahatani kelapa yang lebih baik. Kata kunci: Promecotheca cumingii, kerusakan tanaman, musuh alami. ABSTRACTCoconut pest Promecotheca cumingii Baly (= nucifera Maul.) (Coleoptera: Chrysomelidae) outbreaks in North Sulawesi, firstly occurred in 2015 in Modayag sub district, East Bolaang Mongondow Regency, North Sulawesi Province. Larvae of this leafminers chew and burrow into the leaf tissue. Larvae and pupae spend their entire life inside the leaflets. Adults feed on young leaflets and make characteristic thin longitudinal lines on the lower surfaces of the leaflets. Serious damage can cause scorched fronds and might lead to a significant coconut yield losses. The purposes of this research were to study palm damage, pest population and their natural enemies. Three locations were selected on the Modayag Sub District. In each location 5 palms were selected randomly in the area of the pest attack. At each palm one coconut frond was taken in the middle of the canopy, and the leaf damage, population of larvae, pupae, adult, and natural enemies were measured and counted on the leaflet samples that was taken at every 10 th leaflet. The results showed that the average population of larvae and pup...

show abstract

Prospects of using Metarhizium anisopliae to check the breeding of insect pest, Oryctes rhinoceros L. in coconut leaf vermicomposting sites

Cited by 29 publications

References 8 publications

The growth of tomato seedlings inoculated with co-cultivated Piriformospora indica and Bacillus pumilus

The growth of tomato seedlings inoculated with co-cultivated Piriformospora indica and Bacillus pumilus

Efficacy of Metarhizium anisopliae isolate MAX-2 from Shangri-la, China under desiccation stress

Serangan Hama Kelapa Promecotheca cumingii Baly

Contact Info

Product

Resources

About