Prospects of utilizing seawater as a reaction medium for pretreatment and saccharification of rice straw

Dev, Binita; Bakshi, Arindam; Balasubramanian, P.

doi:10.1016/j.chemosphere.2022.133528

Cited by 13 publications

(3 citation statements)

References 61 publications

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“…Reducing sugar yield enhanced by five folds and 1.5 folds for A and D, respectively as compared to native RS which is due to the degradation of cellulose and hemicellulose 13 . Dev et al 21 used seawater as a reaction medium for microwave-NaOH pretreatment and saccharification of RS. This resulted in cellulose and sugar release of about 65.43% and 0.554 g/g, respectively.…”

Section: Resultsmentioning

confidence: 99%

Rice straw structure changes following green pretreatment with petha wastewater for economically viable bioethanol production

Kumari

Singh

2022

Sci Rep

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Energy efficient and environment friendly pretreatment processes for the production of biofuel have remained elusive and the research is further compounded by the high cost of processing lignocellulosic biomass—an essential factor for producing sustainable biofuels. In the last few decades, a number of pretreatment methods have been proposed, specifically chemical pretreatments but are either expensive or harmful to the environment. To address this urgent need, we propose a green pretreatment method that utilises the highly alkaline by-product, petha wastewater to pretreat the lignocellulosic waste rice straw (RS). The effectiveness of the pretreatment was analysed by monitoring both enhanced cellulose content and reducing sugar yield along with removal of hemicellulose and lignin. We found that PWW pretreatment yielded five times more reducing sugar than native RS with 10.12% increment in cellulose content. SEM and EDX studies further revealed that our process enhanced surface roughness and carbon content (from 32.19% increased to 41.59% and 41.66% for A and D, respectively) along with reduction in silica content (from 8.68% in RS to 4.30% and 7.72% for A and D, respectively). XRD and FTIR analyses indicate crystallinity index (CI) and alteration in lignocellulosic structure of RS, respectively. Decrease in CI was about 43.4% in A whereas only 4.5% in D as compared to native RS (CI 54.55%). Thereby we found PWW to be better substitute of an alkali for pretreatment of RS with negligible environmental impacts.

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Section: Resultsmentioning

confidence: 99%

Rice straw structure changes following green pretreatment with petha wastewater for economically viable bioethanol production

Kumari

Singh

2022

Sci Rep

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“…A similar trend was found in the alkali pretreatment of CS (Table 1). The distortion of surface morphology of biomass by pretreatment can improve the accessibility of enzymes to cellulose [20], and in fact, it was previously found that the enzymatic digestibility of KpCS was improved by approximately 3.1-fold compared to raw CS [12].…”

Section: Discussionmentioning

confidence: 99%

Physicochemical Characterization of Potassium Hydroxide Pretreated Chestnut Shell and Its Bioconversion to Lactic Acid by Lacticaseibacillus rhamnosus

Lee,

Kim,

Son

et al. 2023

Processes

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Lactic acid (LA) is an important platform chemical with a wide range of applications, including bioplastic materials, and demand for it is growing rapidly. However, the high cost of feedstock for LA production is a major barrier to industrial production. This study designed a process to produce LA from chestnut shell (CS), a low-cost biomass. The entire process includes KOH pretreatment, enzymatic saccharification, and fermentation. This study investigated the chemical compositions and physicochemical properties of raw CS and KOH pretreated CS (KpCS) to evaluate the impact of the pretreatment process that enhances the conversion of cellulose into glucose. The results showed that KOH affected the lignin removal and surface morphological changes of CS, and FT-IR and TGA patterns correlated to increased cellulose fractions were found. In the fermentation process, Lacticaseibacillus rhamnosus was selected as a prominent LA producer, and the fermentation using KpCS hydrolysate was carried out. As a result, cell growth (27%), glucose consumption (23%), and LA production (21%) were all achieved higher than the control group. The LA production yield from our suggested process was estimated to be 187 g/kg CS, and we concluded that CS has a high potential as a feedstock for LA production.

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“…Early investigations demonstrating the seawater based biorefining concept have ranged from fermentations targeting ethanol production from Kluyveromyces marxianus and artichoke in seawater mixtures 27 to more conventional approaches using seawater-based sugarcane molasses fermentations 28 . The concept has been assessed in the context of more complex lignocellulosic feedstocks such as rice straw, palm residues, sugarcane bagasse, papaya and the use of macroalgae and microalgae 13,21,[29][30][31][32][33][34] . However, significant further comprehensive research is required to advance the seawater-based biorefinery concept.…”

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confidence: 99%

Functional characterisation of a new halotolerant seawater active glycoside hydrolase family 6 cellobiohydrolase from a salt marsh

Leadbeater,

Bruce

2024

Sci Rep

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Realising a fully circular bioeconomy requires the valorisation of lignocellulosic biomass. Cellulose is the most attractive component of lignocellulose but depolymerisation is inefficient, expensive and resource intensive requiring substantial volumes of potable water. Seawater is an attractive prospective replacement, however seawater tolerant enzymes are required for the development of seawater-based biorefineries. Here, we report a halophilic cellobiohydrolase SMECel6A, identified and isolated from a salt marsh meta-exo-proteome dataset with high sequence divergence to previously characterised cellobiohydrolases. SMECel6A contains a glycoside hydrolase family 6 (GH6) domain and a carbohydrate binding module family 2 (CBM2) domain. Characterisation of recombinant SMECel6A revealed SMECel6A to be active upon crystalline and amorphous cellulose. Mono- and oligosaccharide product profiles revealed cellobiose as the major hydrolysis product confirming SMECel6A as a cellobiohydrolase. We show SMECel6A to be halophilic with optimal activity achieved in 0.5X seawater displaying 80.6 ± 6.93% activity in 1 × seawater. Structural predictions revealed similarity to a characterised halophilic cellobiohydrolase despite sharing only 57% sequence identity. Sequential thermocycling revealed SMECel6A had the ability to partially reversibly denature exclusively in seawater retaining significant activity. Our study confirms that salt marsh ecosystems harbour enzymes with attractive traits with biotechnological potential for implementation in ionic solution based bioprocessing systems.

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Prospects of utilizing seawater as a reaction medium for pretreatment and saccharification of rice straw

Cited by 13 publications

References 61 publications

Rice straw structure changes following green pretreatment with petha wastewater for economically viable bioethanol production

Rice straw structure changes following green pretreatment with petha wastewater for economically viable bioethanol production

Physicochemical Characterization of Potassium Hydroxide Pretreated Chestnut Shell and Its Bioconversion to Lactic Acid by Lacticaseibacillus rhamnosus

Functional characterisation of a new halotolerant seawater active glycoside hydrolase family 6 cellobiohydrolase from a salt marsh

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