ProstaCaid™ inhibits tumor growth in a xenograft model of human prostate cancer

Abstract

Abstract.We have recently demonstrated that the dietary supplement ProstaCaid™ (PC) inhibits growth and invasive behavior of PC-3 human prostate cancer cells in vitro. In the present study, we evaluated toxicity and whether PC suppresses growth of prostate cancer in a xenograft model of human prostate cancer cells implanted in mice. Here, we show that an oral administration of PC (100, 200 and 400 mg/kg) did not affect body weight or activity of liver enzymes (ALT, AST) and did not show any sign of toxicity in liver, spleen, kidney, lung and heart tissues in mice. In addition, PC treatment resulted in the inhibition of tumor volumes (1024.6±378.6 vs. 749.3±234.3, P<0.001) in a xenograft model of prostate cancer with human hormone refractory (independent) PC-3 prostate cancer cells. Moreover, qRT-PCR analysis demonstrated significant upregulation of expression of CDKN1A (p21) and inhibition of expression of IGF2, NR2F2 and PLAU (uPA) genes by an oral administration of PC in prostate cancer xenografts. Our study demonstrates that the concentrations of the dietary supplement ProstaCaid tested did not show signs of toxicity, and its oral application has significant anticancer activity in vivo and can be considered as an alternative treatment for prostate cancer patients.

Comprehensive characterization of chemotherapeutic efficacy on metastases in the established gastric neuroendocrine cancer patient derived xenograft model

“…Statistical analysis was similar to that previously published [ 40 ]. Nonparametric Mann-Whitney U test was applied to compare enumerated metastatic nodules and CTCs in GA0087 PDX mice of vehicle vs cisplatin treated group.…”

Thermally targeted p21 peptide enhances bortezomib cytotoxicity in androgen-independent prostate cancer cell lines

“…Whether overexpressed ectopically or induced by various therapeutic agents p21 WAF1/CIP1 was shown to induce apoptosis in various cancer cell lines as well as xenograft models regardless of p53 status [29,30]. Incubation with the p21-ELP1-Bac polypeptide led to induction of apoptosis only in the DU-145 cell line, which could be the result of a different molecular pathway, since these cells do not have a functional Rb protein.…”

The Nutraceutical Role of Pumpkin Seed and its Health Eff ect: A Review

“…34 Prostate cancer: due to the change of DNA, cells in the prostate develop, especially in women, it has been found that in postmenopausal women, they tend to reduce risk of breast cancer 35 and also in the treatment. 36 It has been said so because the phytoestrogens present in the seeds stimulate estrogen-like behavior, which impacts the proliferation of the cells, contradicting the statement. Still, it helps to prevent breast cancer due to the estrogenic-like effect only.…”

“…10 Furthermore, they also help treat prostate cancer as they can slow down the growth of these cancer cells. 36,37 According to the research, people who consume more lignans are less prone to cancer. They help by blocking those enzymes involved in hormone metabolism and tumor cell growth and spreading.…”