Prostacyclin Causes Splenic Dilation and Haematological Change in Dogs

Abstract

1. The effect of vasodilators on spleen volume and the blood storage function is not yet well elucidated. To this end, in the present study the effects of prostacyclin, a potent vasodilator, on splenic diameter and blood cell concentrations in arterial and splenic venous blood were evaluated in anaesthetized dogs. 2. The main splenic artery and vein were dissected for measurement of splenic arterial blood flow and intra-arterial administration and for sampling of splenic venous blood, respectively. The diameter of the spleen was measured continuously by sonomicrometry. Counts of white blood cells (WBC), red blood cells (RBC) and platelets in blood sampling from the aorta and splenic vein were estimated by an automatic blood cell counter. 3. Bolus injections of prostacyclin (1-100 ng/kg) into the splenic artery produced dose-dependent increases in splenic arterial blood flow and splenic diameter associated with significant decreases in splenic venous concentrations of WBC, RBC and platelets. When splenic blood flow was kept constant, similar changes in splenic diameter and blood cell counts were observed with prostacyclin injection. 4. Splenic dilation and haematological changes induced by prostacyclin were relatively more potent than those induced by prostaglandin E(2), acetylcholine, nitroglycerin or isoproterenol when doses producing a comparable increase in splenic blood flow were compared. 5. Infusion of prostacyclin (100 ng/kg per min) into the splenic artery caused a marked increase in splenic diameter, with immediate reductions in splenic venous concentrations of WBC, RBC and platelets, followed by significant reductions in these cell counts in the general circulation. 6. These results indicate that prostacyclin produces potent and flow-independent splenic dilation that may contribute to a decrease in circulating blood cell concentrations.

The effects of 1,4-dimethylpyridine in metastatic prostate cancer in mice

“…1h–j, Additional File 1: Figure S1e–g). This effect might be attributed to prostacyclin-dependent splenic dilation in the treated mice that leads to white blood cells pooling in the spleen, and in consequence, a systemic decrease in the white blood cell count [23]. The analysis of the biochemical parameters of platelet activity revealed no effect of the studied compounds on platelet activity in TRAMP mice (Fig.…”

Effects of Leukoreduction and Storage on Erythrocyte Phosphatidylserine Expression and Eicosanoid Concentrations in Units of Canine Packed Red Blood Cells

“…Additionally, eicosanoids can modulate the inflammatory response by interacting with signaling molecules, cytokines, and chemokines,16 potentially contributing to the development of transfusion reactions. Although it is unknown how eicosanoids within blood products affect the recipient during transfusion, the infusion of prostacyclin in dogs has been shown to cause immediate and marked vasodilatation 17. Additionally, other eicosanoids in blood products, such as thromboxane, which causes platelet activation and vasoconstriction, could adversely affect the transfusion recipient.…”

“…Although it is unknown how eicosanoids within blood products affect the recipient during transfusion, the infusion of prostacyclin in dogs has been shown to cause immediate and marked vasodilatation. 17 Additionally, other eicosanoids in blood products, such as thromboxane, which causes platelet activation and vasoconstriction, could adversely affect the transfusion recipient.…”