Prostaglandin E2 as stimulator of haemopoietic stem cell proliferation

Fehér, I.; Gidáli, J

doi:10.1038/247550a0

Cited by 84 publications

(32 citation statements)

References 10 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, investigation of these effects directly in the agar cultures is severely impaired by the semisolid agar matrix. The addition of prostaglandin E and acidic isoferritins to agar cultures at time points [12][13][14][15][16][17][18][19][20][21][22][23][24] h after initiation has no effect on CFU-GM proliferation (unpublished observation). This result may occur as a result of loss of CFU-GM Ia-antigen expression (8,11).…”

Section: Discussionmentioning

confidence: 99%

Association between colony forming units-granulocyte macrophage expression of Ia-like (HLA-DR) antigen and control of granulocyte and macrophage production. A new role for prostaglandin E.

Pelus¹

1982

J. Clin. Invest.

View full text Add to dashboard Cite

A B S T R A C T The expression of Ia-like antigens on human colony forming units-granulocyte macrophage (CFU-GM) is related to S-phase of the cell cycle, and associated with the control of normal granulocyte and macrophage production by prostaglandin E and acidic isoferritins in vitro. Ia-antigen expression by CFU-GM is lost within 3-6 h of culture at 370C and occurs simultaneously with loss of responsiveness to inhibition by these factors. Culture of bone marrow CFU-GM in a limited exposure suspension culture with 1 AM-lpM prostaglandin E (PGE1 or PGE2), but not prostaglandin F2a or dibutyryl-cyclic-3'-5'-AMP results in the detection of CFU-GM Ia-antigen after 24 h. Antigen expression is associated with an absolute increase in total and S-phase CFU-GM, and restoration of responsiveness to inhibition by prostaglandin E and acidic isoferritins. The detection of Ia-antigen on CFU-GM after suspension culture with prostaglandin E results both from Iaantigen reexpression as well as stimulation of noncycling cells to enter S-phase, express Ia-antigen and give rise to CFU-GM sensitive to inhibition by prostaglandin E and acidic isoferritins. The sensitivity of CFU-GM to inhibition by these factors after suspension culture with prostaglandin E is identical to that of the same cells tested prior to the suspension culture. These studies provide evidence for a direct regulatory association between Ia-antigen expression and control of myeloid progenitor cell differentiation, and suggest a role for prostaglandin E in the control of CFU-GM cell cycle, Ia-antigen expression, and growth regulation.

show abstract

Section: Discussionmentioning

confidence: 99%

Association between colony forming units-granulocyte macrophage expression of Ia-like (HLA-DR) antigen and control of granulocyte and macrophage production. A new role for prostaglandin E.

Pelus¹

1982

J. Clin. Invest.

View full text Add to dashboard Cite

show abstract

“…Prasad (1972) found that prostaglandin E1, and other agents which raise the cellular level of cyclic adenosine 3',5'-monophosphate (cyclic AMP) reduce in vitro radiation damage. This may be achieved by the depression of cell growth by cyclic AMP. Prostaglandins E could also protect from the effects of radiation by their ability to stimulate haemopoiesis (Feher & Gidali, 1974), and by acting as calcium ionophores (Kirtland & Baum, 1972) …”

Section: Discussionmentioning

confidence: 99%

Effect of Ionizing Radiation on Prostaglandin‐like Activity in Tissues

Eisen

Walker

1976

British J Pharmacology

View full text Add to dashboard Cite

One to 7 days after whole body exposure of mice to a single dose of 700 R of x‐rays, little or no change was detected in prostaglandin‐like activity in the brain, blood and seminal vesicles. Slight increases in intestinal and renal tissue were not significant. In the lung, mean activity rose from 62 ng/g to a transient peak of 145 ng/g wet weight on the fourth day (P > 0.05). In the spleen, mean levels rose steadily from 13.2 ng/g to 259 ng/g on the fourth day (P < 0.01), and were still 184.4 ng/g on the seventh day. Prostaglandin‐like activity was measured 4 days after single doses of 200–700 R. In the lung, a significant rise was produced by 600 and 700 R, and in the spleen by 200–700 R. Thin layer chromatography showed that part of the prostaglandin‐like activity in spleen extracts had an RF similar to that of [3H]‐prostaglandin E1, and part to that of [3H]‐prostaglandin F2α. Splenic tissue from mice exposed to 700 R four days earlier, inactivated prostaglandin E1 less potently than did tissue from non‐irradiated mice.

show abstract

“…Tumor-promoting phorbol esters stimulate the production of prostaglandins by macrophages and prostaglandins have been observed to induce quiescent CFU-S into cell cycle (19).…”

Section: Resultsmentioning

confidence: 99%

Tumor-promoting phorbol esters support the in vitro proliferation of murine pluripotent hematopoietic stem cells.

Spivak¹,

Hogans²,

Stuart³

1989

J. Clin. Invest.

View full text Add to dashboard Cite

The effect of tumor-promoting phorbol esters on the in vitro proliferation of mouse pluripotent hematopoietic stem cells (CFU-S) was examined using a short-term in vitro culture system and an 11-d spleen colony assay. Phorbol myristate acetate (PMA, 10-7 M), but not the inert compound phorbol, supported the in vitro survival of day 11 CFU-S for 72 h in a manner similar to IL 3. PMA also enhanced the effect of IL 3 on the in vitro survival of day 11 CFU-S and as little as 1 h of exposure to PMA was sufficient for this purpose. The effect of PMA on CFU-S survival in vitro was not mediated by prostaglandins, did not require an established adherent cell population, and was observed at a concentration of 10-M. PMA alone did not enhance the in vitro survival of day 11 CFU-S at very low concentrations of FCS but was still able to potentiate the effect of IL 3 on these cells. PMA also enhanced the in vitro survival of day 11 CFU-S from mice treated with 5-fluorouracil or from marrow cells exposed to merocyanine 540 and light. The interaction of PMA with day 11 CFU-S was not inhibited by a neutralizing antiserum to IL 3 but was inhibited by the protein kinase inhibitor H-7. Together, the data indicate that tumor-promoting phorbol esters interact with pluripotent hematopoietic stem cells. Like IL 3, their effect appears to be permissive and involves stem cells with marrow repopulating ability.

show abstract

Prostaglandin E2 as stimulator of haemopoietic stem cell proliferation

Cited by 84 publications

References 10 publications

Association between colony forming units-granulocyte macrophage expression of Ia-like (HLA-DR) antigen and control of granulocyte and macrophage production. A new role for prostaglandin E.

Association between colony forming units-granulocyte macrophage expression of Ia-like (HLA-DR) antigen and control of granulocyte and macrophage production. A new role for prostaglandin E.

Effect of Ionizing Radiation on Prostaglandin‐like Activity in Tissues

Tumor-promoting phorbol esters support the in vitro proliferation of murine pluripotent hematopoietic stem cells.

Contact Info

Product

Resources

About