Prostaglandin E2 Induces Expression of Mineralization Genes by Undifferentiated Dental Pulp Cells

Lorencetti-Silva, Francine; Pereira, Priscilla Aparecida Tártari; Meirelles, Alyne Fávero Galvão; Faccioli, Lúcia Helena; Paula-Silva, Francisco Wanderley Garcia

doi:10.1590/0103-6440201902542

Cited by 11 publications

(9 citation statements)

References 25 publications

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“…LTB 4 showed no cytotoxic to dental pulp cells, measured by the percentage of cell death of less than 30% and in accordance to the International Organization for Standardization guidelines [25]. Other studies that used the PLGA microspheres demonstrated that it is biocompatible and act as particulate adjuvants [17,24,[26][27][28][29]. All these studies showed that microspheres are a viable way to delivery mediators for prolonged time.…”

Section: Discussionmentioning

confidence: 79%

Leukotriene B 4 Loaded in Microspheres Regulate the Expression of Genes Related to Odontoblastic Differentiation and Biomineralization by Dental Pulp Stem Cells

Silva

Lamarque

Oliveira

et al. 2021

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Background: Leukotriene B4 (LTB4) is a potent lipid mediator that stimulate the immune response. Because dental pulp inflammation and dentin repair are intrinsically related responses, the aim of this research was to investigate the potential of LTB4 in inducing differentiation of dental pulp stem cells. Methods: Microspheres (MS) loaded with LTB4 were prepared using an oil emulsion solvent extraction evaporation process and sterility, characterization, efficiency of LTB4 encapsulation and in vitro LTB4 release assay were investigated. Mouse dental pulp stem cells (OD-21) were stimulated with soluble LTB4 or MS loaded with LTB4 (0.01 and 0.1 μM). Cytotoxicity and cell viability was determined by lactate dehydrogenase (LDH) and MTT (methylthiazol tetrazolium) assays . Gene expression were measured by quantitative reverse transcription polymerase chain reaction (qRT-PCR) after 3, 6, 24, 48 and 72 h. Results: Mineralized nodule formation was assessed after 28 days of OD-21 cell stimulation with LTB4. Groups were compared using the one-way ANOVA test followed by Dunnett's post-test (α = 0.05). Treatment with LTB4 or MS loaded with LTB4 (0.01 and 0.1 micrometer- μM) were not cytotoxic to OD-21 cells. Treatment with LTB4 modulated the expression of the Ibsp (integrin binding sialoprotein) and Runx2 (runt-related transcription factor 2) genes differently depending on the experimental period analyzed. Interestingly LTB4 loaded in microspheres (0.1 μM) allowed long term dental pulp cell differentiation and biomineralization. LTB4 loaded in MS was not cytotoxic and induced an odontoblastic cell phenotype differentiation. Conclusion: These findings shed light on a novel pharmacological strategy to induce dental pulp cell differentiation.

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Section: Discussionmentioning

confidence: 79%

Leukotriene B 4 Loaded in Microspheres Regulate the Expression of Genes Related to Odontoblastic Differentiation and Biomineralization by Dental Pulp Stem Cells

Silva

Lamarque

Oliveira

et al. 2021

Preprint

Self Cite

View full text Add to dashboard Cite

show abstract

“…As LTB 4 shows a half-life relatively short, in this study the use of microspheres had the aim to preserve its biological activities a longer time and protect the mediator from degradation [ 24 ]. LTB 4 showed no cytotoxic to dental pulp cells, measured by the percentage of cell death of less than 30% and in accordance to the International Organization for Standardization guidelines [ 25 ].…”

Section: Discussionmentioning

confidence: 99%

“…LTB 4 showed no cytotoxic to dental pulp cells, measured by the percentage of cell death of less than 30% and in accordance to the International Organization for Standardization guidelines [ 25 ]. Other studies that used the PLGA microspheres demonstrated that it is biocompatible and act as particulate adjuvants [ 17 , 24 , 26 – 29 ]. All these studies showed that microspheres are a viable way to delivery mediators for prolonged time.…”

Section: Discussionmentioning

confidence: 99%

Leukotriene B4 loaded in microspheres regulate the expression of genes related to odontoblastic differentiation and biomineralization by dental pulp stem cells

et al. 2022

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Background Leukotriene B4 (LTB4) is a potent lipid mediator that stimulate the immune response. Because dental pulp inflammation and dentin repair are intrinsically related responses, the aim of this research was to investigate the potential of LTB4 in inducing differentiation of dental pulp stem cells. Methods Microspheres (MS) loaded with LTB4 were prepared using an oil emulsion solvent extraction evaporation process and sterility, characterization, efficiency of LTB4 encapsulation and in vitro LTB4 release assay were investigated. Mouse dental pulp stem cells (OD-21) were stimulated with soluble LTB4 or MS loaded with LTB4 (0.01 and 0.1 μM). Cytotoxicity and cell viability was determined by lactate dehydrogenase and methylthiazol tetrazolium assays. Gene expression were measured by quantitative reverse transcription polymerase chain reaction after 3, 6, 24, 48 and 72 h. Mineralized nodule formation was assessed after 28 days of OD-21 cell stimulation with LTB4 in mineralized media or not. Groups were compared using one-way ANOVA test followed by Dunnett’s post-test (α = 0.05). Results Treatment with LTB4 or MS loaded with LTB4 (0.01 and 0.1 µm-μM) were not cytotoxic to OD-21 cells. Treatment with LTB4 modulated the expression of the Ibsp (integrin binding sialoprotein) and Runx2 (runt-related transcription factor 2) genes differently depending on the experimental period analyzed. Interestingly LTB4 loaded in microspheres (0.1 μM) allowed long term dental pulp cell differentiation and biomineralization. Conclusion LTB4, soluble or loaded in MS, were not cytotoxic and modulated the expression of the Ibsp and Runx2 genes in cultured OD-21 cells. When LTB4 was incorporated into MS, odontoblast differentiation and mineralization was induced in long term culture.

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“…On the other hand, studies have shown that PGE 2 inhibits the osteogenic differentiation of mesenchymal stem cells (Ern et al, 2017). In addition, PGE 2 induces the expression of genes associated with mineralization by undifferentiated cells of the dental pulp (Lorencetti‐Silva et al, 2019), improving the formation of tertiary dentine (Limjeerajarus et al, 2014). Various studies carried out on human dental pulps have reported that, during pulp inflammation, the concentration of PGE 2 in dental pulps with pulpitis (Nakanishi et al, 1995) significantly increases, both reversible (Cohen et al, 1985; Petrini et al, 2012) and irreversible (Cohen et al, 1985), compared to healthy dental pulps.…”

Section: Discussionmentioning

confidence: 99%

Effect of treatment with resiniferatoxin in an experimental model of pulpal inflammatory in mice

et al. 2021

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Aim To evaluate whether treatment with resiniferatoxin (RTX) is capable of lowering the plasma levels of PGE2 and TNF‐α, as well as histopathological parameters in inflammation of pulp tissue in a mouse experimental model. Methodology Ten groups of six BALB/c mice were formed as follows: healthy group (HC), healthy group treated with RTX (HRTX), two groups with pulp inflammation at 14 and 18 hours (PI14/PI18), six groups with pulpal inflammation plus treatment with Ibuprofen (IBU14/IBU18), dexamethasone (DEX14/DEX18) and resiniferatoxin (RTX14/RTX18) at 14 and 18 hours, respectively. Pulpal inflammation was induced through occlusal exposure of the pulp of the maxillary first molar. The plasma levels of PGE2 and TNF‐α and the histological parameters of the pulp tissue of the HC and HRTX groups were evaluated at the time of acquiring the animals. In the other groups, the plasma levels of PGE2 and TNF‐α and the histopathological parameters were evaluated at 14 and 18 hours after pulp damage. Plasma levels of PGE2 and TNF‐α were quantified by ELISA, and the histopathological parameters were evaluated by H/E staining. Statistical significance was determined by one‐way analysis of variance (ANOVA) to test for overall differences between group means. Results A significant increase (*p < .05) in plasma levels of PGE2 and TNF‐α occurred 14 and 18 hours after pulp damage. In addition, treatment with RTX significantly decreased (*p < .05) the plasma levels of PGE2 and TNF‐α at 14 and 18 hours after pulp damage, as well as the infiltrate of inflammatory cells at 18 hours after pulp damage, similarly to treatment with ibuprofen and dexamethasone. Conclusion It was possible to detect systemic levels of PGE2 and TNF‐α at 14 and 18 hours after pulp damage. Likewise, treatment with RTX was associated with an anti‐inflammatory effect similar to treatment with ibuprofen and dexamethasone. These findings place resiniferatoxin as a therapeutic alternative in the treatment of inflammatory diseases in Dentistry.

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Prostaglandin E2 Induces Expression of Mineralization Genes by Undifferentiated Dental Pulp Cells

Cited by 11 publications

References 25 publications

Leukotriene B 4 Loaded in Microspheres Regulate the Expression of Genes Related to Odontoblastic Differentiation and Biomineralization by Dental Pulp Stem Cells

Leukotriene B 4 Loaded in Microspheres Regulate the Expression of Genes Related to Odontoblastic Differentiation and Biomineralization by Dental Pulp Stem Cells

Leukotriene B4 loaded in microspheres regulate the expression of genes related to odontoblastic differentiation and biomineralization by dental pulp stem cells

Effect of treatment with resiniferatoxin in an experimental model of pulpal inflammatory in mice

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