Prostaglandin E2`nin Koyunlarda Erken Luteal Dönemde Korpus Luteum Üzerine Etkisi

Abstract

Prostaglandins (PGs) in particular PGE2 by regulating luteal angiogenesis and steroidogenesis, play an important role in ovarian as well as luteal function in many mammalian species. While the direct luteotrophic effect of PGE2 has been studied intensively, there is still not much information available regarding the indirect effects of PGE2 in ovine corpus luteum (CL). Therefore, in the present study, possible effects of PGE2 stimulation on expression of some steroidogenic hormone receptors (ERα/ER1, ERβ/ER2 and PGR) and growth factors (IGF1, IGF2 and VEGF) were investigated using the isolated primary luteal and -microvascular endothelial cells. Neither the expression of steroidogenic hormone receptors (ERα/ER1, ERβ/ER2 and PGR) nor growth factors (IGF1, IGF2 and VEGF) were affected by PGE2 stimulation in primary luteal cells. However, there was statistically significant difference for the expression of both ERα/ER1 and ERβ/ER2 following PGE2 treatment in primary microvascular endothelial cells. Moreover, while the expression of IGF2 was upregulated, there was no statistically significant change for the expression of IGF1 and VEGF following PGE2 treatment in ovine primary microvascular endothelial cells. In conclusion, by regulating expression of estrogen receptors (ERα/ER1 and ERβ/ ER2) and IGF2 in ovine luteal endothelial cells, PGE2 might involve in luteal angiogenesis and vasculogenesis during early luteal phase in sheep.