Prostaglandin F2-alpha receptor (FPr) expression on porcine corpus luteum microvascular endothelial cells (pCL-MVECs)

Zannoni, Augusta; Bernardini, Chiara; Rada, Tommaso; Ribeiro, Luciana De Andrea; Forni, Monica; Bacci, Maria Laura

doi:10.1186/1477-7827-5-31

Cited by 17 publications

(15 citation statements)

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“…Although FPr mRNA has been demonstrated to be expressed in cultured LECs isolated from the CLs of cycling pigs [39] and cows [9], some studies have reported the absence of FPr in bovine LECs isolated from pregnant CLs [7,40]. The differences observed in FPr mRNA expression may be due to differences in the physiological stages of the CL, culture systems, types of LEC or immunostaining techniques as described previously.…”

Section: Discussionmentioning

confidence: 85%

“…The differences observed in FPr mRNA expression may be due to differences in the physiological stages of the CL, culture systems, types of LEC or immunostaining techniques as described previously. FPr protein has recently been identified on cultured porcine LECs [39]. However, the presence of functional FPr in LECs isolated from bovine CLs has not been reported.…”

Section: Discussionmentioning

confidence: 99%

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Prostaglandin F2α Regulates the Nitric Oxide Generating System in Bovine Luteal Endothelial Cells

Lee

Acosta

Yoshioka

et al. 2009

Journal of Reproduction and Development

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Abstract. The objective of the present study was to elucidate whether luteolytic prostaglandin F2α (PGF) plays roles in regulating the nitric oxide (NO) generating system in luteal endothelial cells (LECs). Reverse transcriptase PCR, immunoblotting and immunostaining revealed the presence of PGF receptor mRNA (521 bp) and protein (64 kDa) in cultured LECs obtained from the mid-stage corpus luteum. When cultured LECs were exposed to 0.1 μM-10 μM PGF, NO production was significantly stimulated by PGF at 24 h. When LECs were exposed to 1 μM PGF for 2, 6 and 24 h, PGF did not affect the expressions of endothelial NO synthase (eNOS) mRNA and protein. On the other hand, PGF stimulated the expression of inducible NOS (iNOS) mRNA (P<0.05) and protein (P<0.05) at 2 h, but not at 6 and 24 h. By observing the conversion of [ 3 C]L-arginine to [ 3 C]L-citrulline, we found that PGF stimulated NOS activity in cultured LECs at 2 h (P<0.05). The overall findings indicate that bovine LECs are a target for PGF and that PGF stimulates iNOS expression and NOS activity in bovine LECs. Stimulation of the NO generating system and NOS activity by PGF may result in increasing local NO production followed by luteolysis. Key words: eNOS, iNOS, Luteal endothelial cell, Nitric oxide, Prostaglandin F2α (PGF2α) (J. Reprod. Dev. 55: [418][419][420][421][422][423][424] 2009) he corpus luteum (CL) is a transient endocrine gland essential for regulation of ovarian cycles as well as for establishment and maintenance of pregnancy. If pregnancy does not occur, the bovine corpus luteum starts to regress between days 17-19 after ovulation [1]. Functional and structural regression of the CL in mammals is induced by the pulsatile release of prostaglandin F2α(PGF) from the uterus and the ovary [2][3][4]. Although regarded as a physiological luteolysin in the cow, the local mechanism involved in the luteolytic action of PGF remains unclear.The actions of PGF are mediated by the PGF receptor (FPr) located in the plasma membrane. Bovine luteal cell membranes have the ability to bind PGF throughout the estrous cycle [5,6]. However, there is no consensus on the presence of FPr in the bovine luteal vasculature. Cavicchio et al. reported the absence of FPr mRNA in luteal endothelial cells (LECs) isolated from early pregnant cows [7]. More recent studies using endothelial cells (ECs) derived from cyclic bovine CLs have demonstrated that freshly isolated and cultured LECs express FPr mRNA [8,9]. Furthermore, FPr has been immunohistologically demonstrated to be expressed in luteal tissue including steroidogenic cells and large blood vessels in the peripheral region of the mature CL [10].Directly treating pure populations of luteal steroidogenic cells with PGF does not inhibit basal progesterone (P4) production [11,12], although intramuscular injections of PGF analogues induce a rapid decrease in P4 production [13]. Similarly, a decrease in P4 release has been observed when bovine luteal cells (LCs) were exposed to PGF in the presence of endothelin-1 (EDN1) [14,...

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Section: Discussionmentioning

confidence: 85%

Section: Discussionmentioning

confidence: 99%

Prostaglandin F2α Regulates the Nitric Oxide Generating System in Bovine Luteal Endothelial Cells

Lee

Acosta

Yoshioka

et al. 2009

Journal of Reproduction and Development

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“…Since PGF 2a also augmented the content of CREB in CL of early pregnant pigs, it seems likely that PGF 2a is responsible for enhancement of cAMP action, and can also increase availability of CREB which is activated further by luteotropic hormones acting via cAMP, e.g., PGE 2 and LH. While expression of PTGFR was prominent in endothelial cells (Zannoni et al 2007) of cyclic CL, we postulate that PGF 2a might also be engaged in luteal function maintenance during early pregnancy in pigs by stimulation of angiogenesis. A positive effect of PGF 2a on angiogenesis was previously demonstrated in bovine CL (Shirasuna et al 2012, Zalman et al 2012.…”

Section: Rescue Of Luteal Function During Early Pregnancymentioning

confidence: 99%

Steroid hormones, prostanoids, and angiogenic systems during rescue of the corpus luteum in pigs

Przygrodzka¹,

Kaczmarek²,

Kaczyński³

et al. 2016

Reproduction

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In order to characterize the transition of the corpora lutea (CL) from acquisition of luteolytic sensitivity to rescue of luteal function: i) the expression of 38 factors associated with steroids, prostanoids, and angiogenic systems and ii) concentrations of the main hormones responsible for maintenance of CL function in cyclic and pregnant pigs were examined. Additionally, the effect of prostaglandin (PG) E 2 and F 2a on luteal function during the estrous cycle and pregnancy was evaluated in vitro. Significantly up-regulated gene expression was revealed in CL collected on day 14 of the estrous cycle (CYP19A1, ESR2, PTGS2, HIF1A, and EDN1) and on days 12-14 of pregnancy (SCARB1, PGRMC1, STAR, HSD3B1, NR5A1, PTGFR, PTGER4, and VEGFA). Elevated concentrations of estradiol-17b and PGE 2 occurred in CL on days 12 and 14 of pregnancy respectively, while an increased intraluteal PGF 2a content was noted on day 14 of the estrous cycle. Both PGs increased the synthesis of progesterone by cultured luteal slices obtained on day 14 of pregnancy, in contrast to the action of PGF 2a on the corresponding day of the estrous cycle. PGE 2 stimulated cAMP production via PTGER2 and PTGER4, while PGF 2a elevated the content of CREB in cultured luteal slices from CL of pregnant pigs. In silico analysis showed that infiltration of lymphocytes and apoptosis of microvascular endothelium were activated in CL on day 12 of the estrous cycle vs pregnancy. Summarizing, an abundance of E 2 and PGE 2 during pregnancy regulates specific pathways responsible for steroidogenesis, the prostanoid signaling system and angiogenesis during rescue from luteolysis in porcine CL.

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“…gyrB was used to normalize for transcript quantification, because it has been shown to be not affected by cell density and/or agr (13). Relative RNAIII expression was calculated as the differences in cycle thresholds (⌬C T ) (gyrB C T Ϫ RNAIII C T ) for all samples as previously described (25,47,53). PCR experiments were performed using two biological replicates, each tested in triplicate.…”

Section: Methodsmentioning

confidence: 99%

Relationship of agr Expression and Function with Virulence and Vancomycin Treatment Outcomes in Experimental Endocarditis Due to Methicillin-Resistant Staphylococcus aureus

Seidl

Chen

Bayer

et al. 2011

Antimicrob Agents Chemother

102

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Prostaglandin F2-alpha receptor (FPr) expression on porcine corpus luteum microvascular endothelial cells (pCL-MVECs)

Cited by 17 publications

References 50 publications

Prostaglandin F<sub>2</sub><sub>α</sub> Regulates the Nitric Oxide Generating System in Bovine Luteal Endothelial Cells

Prostaglandin F<sub>2</sub><sub>α</sub> Regulates the Nitric Oxide Generating System in Bovine Luteal Endothelial Cells

Steroid hormones, prostanoids, and angiogenic systems during rescue of the corpus luteum in pigs

Relationship of agr Expression and Function with Virulence and Vancomycin Treatment Outcomes in Experimental Endocarditis Due to Methicillin-Resistant Staphylococcus aureus

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Prostaglandin F2-alpha receptor (FPr) expression on porcine corpus luteum microvascular endothelial cells (pCL-MVECs)

Cited by 17 publications

References 50 publications

Prostaglandin F<sub>2</sub><sub>&alpha;</sub> Regulates the Nitric Oxide Generating System in Bovine Luteal Endothelial Cells

Prostaglandin F<sub>2</sub><sub>&alpha;</sub> Regulates the Nitric Oxide Generating System in Bovine Luteal Endothelial Cells

Steroid hormones, prostanoids, and angiogenic systems during rescue of the corpus luteum in pigs

Relationship of agr Expression and Function with Virulence and Vancomycin Treatment Outcomes in Experimental Endocarditis Due to Methicillin-Resistant Staphylococcus aureus

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Product

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Prostaglandin F<sub>2</sub><sub>α</sub> Regulates the Nitric Oxide Generating System in Bovine Luteal Endothelial Cells

Prostaglandin F<sub>2</sub><sub>α</sub> Regulates the Nitric Oxide Generating System in Bovine Luteal Endothelial Cells