Prostaglandin F2  increases endothelial nitric oxide synthase in the periphery of the bovine corpus luteum: the possible regulation of blood flow at an early stage of luteolysis

Shirasuna, Koumei; Watanabe, Sho; Asahi, Takayuki; Wijayagunawardane, Missaka P.B.; Sasahara, Kiemi; Jiang, Chao; Matsui, Motozumi; Sasaki, Motoki; Shimizu, Takashi; Davis, John S.; Miyamoto, Akio

doi:10.1530/rep-07-0496

Cited by 58 publications

(64 citation statements)

References 72 publications

(74 reference statements)

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“…Direct inhibitory and stimulating effects of PGF on small and large luteal cells respectively have been demonstrated in vitro (Alila et al 1988, Meidan et al 1992, Girsh et al 1995, Okuda et al 1998, Skarzynski & Okuda 1999. In addition, the results of studies involving treatment with a bolus luteolytic dose of PGF have been interpreted to indicate that nitric oxide is a crucial factor in initiating luteolysis through a drastic increase in luteal blood flow (Shirasuna et al 2008), and that the induced increase in luteal blood flow is the first step in the luteolytic cascade (Miyamoto et al 2005). However, a direct functional role of the acute increase in luteal blood flow in the ensuing luteolysis has not been demonstrated.…”

Section: Discussionmentioning

confidence: 99%

“…Luteal blood flow or the percentage of CL area with blood flow colour displays was estimated from the real-time sequential two-dimensional planes of the entire CL. The colour flow signals at the periphery of the CL and within the CL were included in the percentage estimates; previous study has shown major involvement of the peripheral vessels during luteolysis (Shirasuna et al 2008). The subjective estimation of the percentage of CL area with blood flow signals as an end point in cattle has been described in detail and validated with independent operators and objectively by using coloured pixels in still images (Ginther 2007, Ginther et al 2007b, Araujo & Ginther 2009).…”

Section: Ultrasound Scanning and Luteal Blood Flow Estimationmentioning

confidence: 99%

“…routes of PGF treatment in various doses (Ginther et al 2007b(Ginther et al , 2009b. In addition, based on either 12-or 24-h intervals between examinations (Miyamoto et al 2005, Shirasuna et al 2008, the progesterone decrease associated with spontaneous luteolysis was reported to be preceded by increased blood flow to the corpus luteum (CL). It has therefore been postulated that an increase in luteal blood flow is an initial event in the PGF-induced luteolytic cascade ).…”

Section: Introductionmentioning

confidence: 99%

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Luteal blood flow and concentrations of circulating progesterone and other hormones associated with a simulated pulse of 13,14-dihydro-15-keto-prostaglandin F2α in heifers

Shrestha¹,

Beg²,

Imam³

et al. 2010

Reproduction

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Progesterone and luteal blood flow effects of an i.u. 2-h infusion of 0.25 mg/h of prostaglandin F 2a (PGF) that simulated a natural pulse of 13,14-dihydro-15-keto-PGF (PGFM) were compared to the effects of a single bolus i.u. injection of PGF (4 mg) that induced complete luteolysis in heifers. Blood sampling and an estimate of the percentage of luteal area with colour-Doppler signals of blood flow were performed every 2 min for 20 min and less frequently thereafter for 6 h. After the beginning of PGF infusion or a bolus injection, progesterone increased to a peak at 14 and 10 min respectively, and was accompanied by an increase in blood flow in the bolus group but not in the infusion group. Progesterone then decreased for 1 or 2 h and was accompanied by a continued elevation in blood flow in the PGF bolus group and by a slight increase in the PGF infusion group. Progesterone then rebounded in both groups, but the rebound was greater in the infusion group. Blood flow decreased during the descending arm of the progesterone rebound. Cortisol and prolactin began to increase 6 min after the bolus PGF injection but did not increase during or after PGF infusion. The increases in cortisol, prolactin and blood flow after a PGF bolus treatment but not during a simulated PGFM pulse indicated that the bolus treatment was pharmacologic, and its use may lead to faulty conclusions on the nature of physiologic luteolysis. The comparisons between progesterone and blood flow are novel.

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Section: Discussionmentioning

confidence: 99%

Section: Ultrasound Scanning and Luteal Blood Flow Estimationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Luteal blood flow and concentrations of circulating progesterone and other hormones associated with a simulated pulse of 13,14-dihydro-15-keto-prostaglandin F2α in heifers

Shrestha¹,

Beg²,

Imam³

et al. 2010

Reproduction

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“…The CL was enucleated from the ovary and dissected free of connective tissue as previously described (Shirasuna et al 2008a). Subsequently, the CL cut into a cube shape (about 5 mm), was fixed with 10% formaldehyde for 24 h at room temperature and embedded in paraffin wax according to the standard histological technique.…”

Section: Processing Of the CLmentioning

confidence: 99%

Possible role of interferon tau on the bovine corpus luteum and neutrophils during the early pregnancy

et al. 2015

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When pregnancy is established, interferon tau (IFNT), a well-known pregnancy recognition signal in ruminants, is secreted by embryonic trophoblast cells and acts within the uterus to prepare for pregnancy. IFNT acts as an endocrine factor on the corpus luteum (CL) to induce refractory ability against the luteolytic action of PGF 2a . Hypothesising that IFNT may influence not only the uterine environment but also the CL in cows via local or peripheral circulation, we investigated qualitative changes in the CL of pregnant cows during the maternal recognition period (day 16) and the CL of non-pregnant cows. The CL of pregnant animals had a higher number of neutrophils, and the expression of interleukin 8 (IL8) mRNA and its protein was higher as well as compared with the CL of non-pregnant animals. Although IFNT did not affect progesterone (P 4 ) secretion and neutrophil migration directly, it stimulated IL8 mRNA expression on luteal cells (LCs), influencing the neutrophils, resulting in the increased migration of IFNT-activated neutrophils. Moreover, both IFNT-activated neutrophils and IL8 increased P 4 secretion from LCs in vitro. Our novel finding was the increase in neutrophils and IL8 within the CL of pregnant cows, suggesting the involvement of IFNT function within the CL toward establishment of pregnancy in cows. The present results suggest that IFNT upregulates neutrophil numbers and function via IL8 on LCs in the CL of early pregnant cows and that both neutrophils and IL8, stimulated by IFNT, are associated with an increase in P 4 concentrations during the maternal recognition period in cows. Reproduction (2015) 150 217-225

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“…NO may be an important mediator of luteolysis in the cow [19,20], and in the rat the evidence was provided for the existence a positive feedback mechanism between PGF2α and NOS in the CL [23]. To investigate the relationships between increase of luteal blood flow by PGF2α injection and NO (especially eNOS), we collected the CL both from the early and mid luteal phase at 30 min after PGF2α administration in the cow [41]. Consistent with a lack of effect of PGF2α on luteal blood flow in the early CL, PGF2α did not increase eNOS mRNA and immunostaining in the early CL (Fig.…”

Section: Nitric Oxide In the Luteal Blood Flow And Initiation Of Lutementioning

confidence: 99%

Nitric Oxide and Luteal Blood Flow in the Luteolytic Cascade in the Cow

Shirasuna

2010

Journal of Reproduction and Development

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Abstract. The corpus luteum (CL) of the estrous cycle in the cow is a dynamic organ which has a lifespan of approximately 17-18 days. The main function of the CL is to produce progesterone (P) that requires for achievement and maintenance of pregnancy. If pregnancy does not occur successfully, the CL must regress within a few days to induce the next chance of ovulation. As the CL matures, the steroidogenic cells establish contact with many capillary vessels, consequently the CL is composed of a large number of vascular endothelial cells that can account for up to 50% of all cells. Therefore, blood vessels and blood flow within the CL have an essential role in luteal function. Nitric oxide (NO), a strong vasorelaxant, is now known to play key roles in a variety of physiological process. Indeed, NO has established itself as a polyvalent molecule which plays a decisive role in regulating multiple functions within female reproductive system. In the CL, NO is produced and regulates luteal blood flow, P secretion and apoptosis of luteal cells as well as endothelial cells. This review describes the current investigation for possible roles of NO in the luteolytic cascade within the bovine CL. Key words: Corpus luteum, Luteal blood flow, Luteolysis, Nitric oxide (J. Reprod. Dev. 56: [9][10][11][12][13][14] 2010) he corpus luteum (CL) is a transient organ in the ovary of mammals. The bovine CL rapidly develops within 2-3 days after ovulation, which is accompanied by an active angiogenesis and vascularization from the preovulatory follicle. The main function of the CL is to produce progesterone (P) that requires for achievement and maintenance of pregnancy [1]. If pregnancy does not occur successfully, the CL is only functional for 17-18 days and it must regress within a few days to induce the next chance of ovulation. In ruminants, it is well known that a pulsatile release of prostaglandin F2α (PGF2α) from the uterus on days 17-18 of the estrous cycle is essential to induce regression of the CL [2]. Luteolytic PGF2α induces a drastic decrease in P release from the CL as well as CL volume and blood flow to the CL in the non-pregnant cow [3,4]. Thus, it is easily considered that the bovine CL has well designed mechanisms by which development and regression are effectively controlled.In the cow, the CL is composed of a large number of vascular endothelial cells that can account for up to 50% of all the CL cells [5]. On the other hand, steroidogenic large luteal cells and small luteal cells constitute about 30% of all CL cells [5,6]. The bovine CL also has various cell types such as smooth muscle cells, pericytes, fibrocytes and immune cells, indicating that the CL is a heterogeneous tissue [7,8]. In addition, the CL is constituted by the condensed blood vasculature including large blood vessels and microcapillaries, and the majority of steroidogenic cells are adjacent to one or more capillaries [9]. It is known that a cell-to-cell interaction and these adhesions are essentially important for the CL integrity and physiolog...

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Prostaglandin F2 increases endothelial nitric oxide synthase in the periphery of the bovine corpus luteum: the possible regulation of blood flow at an early stage of luteolysis

Cited by 58 publications

References 72 publications

Luteal blood flow and concentrations of circulating progesterone and other hormones associated with a simulated pulse of 13,14-dihydro-15-keto-prostaglandin F2α in heifers

Luteal blood flow and concentrations of circulating progesterone and other hormones associated with a simulated pulse of 13,14-dihydro-15-keto-prostaglandin F2α in heifers

Possible role of interferon tau on the bovine corpus luteum and neutrophils during the early pregnancy

Nitric Oxide and Luteal Blood Flow in the Luteolytic Cascade in the Cow

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