Prostaglandins and the Zone of Calcified Cartilage in Osteoarthritis

Oegema, Theodore R.; Johnson, Sharon L.; Meglitsch, Toni; Carpenter, Randall J.

doi:10.1097/00045391-199602000-00008

Cited by 24 publications

(38 citation statements)

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“…This region was selected for cell isolation for two reasons. First, it contains cells with alkaline phosphatase activity, an enzyme which is involved in mineralization (35) and second, this region of cartilage can undergo mineralization in vivo, indicating that the ceils have the potential to synthesize matrix components that favor mineralization under the appropriate conditions (17,18).…”

Section: Discussionmentioning

confidence: 99%

“…The calcified cartilage interfaces between the articular cartilage and the subchondral bone in synovial type joints (17,18). Little is known about the composition of the calcified cartilage and the metabolism of chondrocytes within and immediately above this zone (14,18).…”

mentioning

confidence: 99%

“…Little is known about the composition of the calcified cartilage and the metabolism of chondrocytes within and immediately above this zone (14,18). With the remodeling of the calcified cartilage that occurs normally (13,29) as well as in joint diseases such as osteoarthritis (17,18), the deep zone of the hyaline cartilage can undergo mineralization. Animal studies have suggested that damage to the calcified cartilage can result in the development of osteoarthritis (27) and human radiological studies have suggested that damage to this region of joint is relatively common (28).…”

mentioning

confidence: 99%

“…channels (20). The calcified zone of articular cartilage does not usually undergo vascular invasion unless there is an underlying disease process (17,18).…”

mentioning

confidence: 99%

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In vitro formation of mineralized cartilagenous tissue by articular chondrocytes

Kandel

Boyle

Gibson

et al. 1997

In Vitro Cell.Dev.Biol.-Animal

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Study of the deep articular cartilage and adjacent calcified cartilage has been limited by the lack of an in vitro culture system which mimics this region of the cartilage. In this paper we describe a method to generate mineralized cartilagenous tissue in culture using chondrocytes obtained from the deep zone of bovine articular cartilage. The cells were plated on Millipore CMR filters. The chondrocytes in culture accumulated extracellular matrix and formed cartilagenous tissue which calcified when beta-glycerophosphate was added to the culture medium. The cartilagenous tissue generated in vitro contains both type II and type X collagens, large sulfated proteoglycans, and alkaline phosphatase activity. Ultrastructurally, matrix vesicles were seen in the extracellular matrix. Selected area electron diffraction confirmed that the calcification was composed of hydroxyapatite crystals. The chondrocytes, as characterized thus far, appear to maintain their phenotype under these culture conditions which suggests that these cultures could be used as a model to examine the metabolism of cells from the deep zone of cartilage and mineralization of cartilagenous tissue in culture.

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Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

“…channels (20). The calcified zone of articular cartilage does not usually undergo vascular invasion unless there is an underlying disease process (17,18).…”

mentioning

confidence: 99%

See 2 more Smart Citations

In vitro formation of mineralized cartilagenous tissue by articular chondrocytes

Kandel

Boyle

Gibson

et al. 1997

In Vitro Cell.Dev.Biol.-Animal

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“…19,54,67 Advancement of the calcified region towards the articular surface is observed with age, 30,42,43,60,62 and has been associated with osteoarthritis. 11,12,14,61,63,65 According to Collins, the absence of a stable calcified cartilage interface between the cartilage proper and vascular bone, whether in a joint, an intervertebral disc or a rib, indicates that the interface is temporary, unstable and often pathological. 19 Hunziker et al 35,36 elegantly demonstrated that a physical barrier is essential for maintaining the stability of the neo-cartilage formed post repair and would prevent unwanted bony ingrowth.…”

Section: Introductionmentioning

confidence: 99%

Bioactive Stratified Polymer Ceramic-Hydrogel Scaffold for Integrative Osteochondral Repair

et al. 2010

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Due to the intrinsically poor repair potential of articular cartilage, injuries to this soft tissue do not heal and require clinical intervention. Tissue engineered osteochondral grafts offer a promising alternative for cartilage repair. The functionality and integration potential of these grafts can be further improved by the regeneration of a stable calcified cartilage interface. This study focuses on the design and optimization of a stratified osteochondral graft with biomimetic multi-tissue regions, including a pre-designed and pre-integrated interface region. Specifically, the scaffold based on agarose hydrogel and composite microspheres of polylactide-co-glycolide (PLGA) and 45S5 bioactive glass (BG) was fabricated and optimized for chondrocyte density and microsphere composition. It was observed that the stratified scaffold supported the region-specific co-culture of chondrocytes and osteoblasts which can lead to the production of three distinct yet continuous regions of cartilage, calcified cartilage and bone-like matrices. Moreover, higher cell density enhanced chondrogenesis and improved graft mechanical property over time. The PLGA-BG phase promoted chondrocyte mineralization potential and is required for the formation of a calcified interface and bone regions on the osteochondral graft. These results demonstrate the potential of the stratified scaffold for integrative cartilage repair and future studies will focus on scaffold optimization and in vivo evaluations.

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The interaction of the zone of calcified cartilage and subchondral bone in osteoarthritis

Oegema

Carpenter

Hofmeister

et al. 1997

Microsc. Res. Tech.

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222

175

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Prostaglandins and the Zone of Calcified Cartilage in Osteoarthritis

Cited by 24 publications

References 0 publications

In vitro formation of mineralized cartilagenous tissue by articular chondrocytes

In vitro formation of mineralized cartilagenous tissue by articular chondrocytes

Bioactive Stratified Polymer Ceramic-Hydrogel Scaffold for Integrative Osteochondral Repair

The interaction of the zone of calcified cartilage and subchondral bone in osteoarthritis

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