Prostaglandins temporally regulate cytoplasmic actin bundle formation during<i>Drosophila</i>oogenesis

Spracklen, Andrew J.; Kelpsch, Daniel J.; Chen, Xiang; Spracklen, Cassandra N.; Tootle, Tina L.

doi:10.1091/mbc.e13-07-0366

Cited by 48 publications

(68 citation statements)

References 80 publications

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“…2D–F”, orange boxed regions); this suggests that GFP-Utrophin expression may stabilize and/or promote such cytoskeletal remodeling. Interestingly, similar, but less severe early actin structures are observed when PG signaling is lost (Spracklen et al, 2014). …”

Section: Resultsmentioning

confidence: 83%

“…Occasionally, more extensive actin structures, including elongated actin filament bundles and aggregates, are observed (Fig. 1B-B’, yellow arrows) (Spracklen et al, 2014). At S10A, these “early” actin structures are no longer apparent (Fig.…”

Section: Resultsmentioning

confidence: 99%

“…Little actin remodeling is seen within the nurse cells during these stages. Intriguingly, we have observed that some remodeling is occurring at the ring canals connected to the oocyte in the posterior nurse cells during stage 9 (S9) (Spracklen et al, 2014). This normally results in minimal filaments near the ring canals (see Fig.…”

Section: Introductionmentioning

confidence: 82%

“…1B-B’). Such remodeling is regulated as we have found that genetic loss of the Drosophila COX-like enzyme, Pxt, results in a significant increase in the prevalence of these early actin structures (Spracklen et al, 2014). …”

Section: Introductionmentioning

confidence: 99%

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The pros and cons of common actin labeling tools for visualizing actin dynamics during Drosophila oogenesis

Spracklen¹,

Fagan²,

Lovander³

et al. 2014

Developmental Biology

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122

110

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Dynamic remodeling of the actin cytoskeleton is required for both development and tissue homeostasis. While fixed image analysis has provided significant insight into such events, a complete understanding of cytoskeletal dynamics requires live imaging. Numerous tools for the live imaging of actin have been generated by fusing the actin-binding domain from an actin-interacting protein to a fluorescent protein. Here we comparatively assess the utility of three such tools – Utrophin, Lifeact, and F-tractin – for characterizing the actin remodeling events occurring within the germline-derived nurse cells during Drosophila mid-oogenesis or follicle development. Specifically, we used the UAS/GAL4 system to express these tools at different levels and in different cells, and analyzed these tools for effects on fertility, alterations in the actin cytoskeleton, and ability to label filamentous actin (F-actin) structures by both fixed and live imaging. While both Utrophin and Lifeact robustly label F-actin structures within the Drosophila germline, when strongly expressed they cause sterility and severe actin defects including cortical actin breakdown resulting in multi-nucleate nurse cells, early F-actin filament and aggregate formation during stage 9 (S9), and disorganized parallel actin filament bundles during stage 10B (S10B). However, by using a weaker germline GAL4 driver in combination with a higher temperature, Utrophin can label F-actin with minimal defects. Additionally, strong Utrophin expression within the germline causes F-actin formation in the nurse cell nuclei and germinal vesicle during mid-oogenesis. Similarly, Lifeact expression results in nuclear F-actin only within the germinal vesicle. F-tractin expresses at a lower level than the other two labeling tools, but labels cytoplasmic F-actin structures well without causing sterility or striking actin defects. Together these studies reveal how critical it is to evaluate the utility of each actin labeling tool within the tissue and cell type of interest in order to identify the tool that represents the best compromise between acceptable labeling and minimal disruption of the phenomenon being observed. In this case, we find that F-tractin, and perhaps Utrophin, when Utrophin expression levels are optimized to label efficiently without causing actin defects, can be used to study F-actin dynamics within the Drosophila nurse cells.

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Section: Resultsmentioning

confidence: 83%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 82%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

The pros and cons of common actin labeling tools for visualizing actin dynamics during Drosophila oogenesis

Spracklen¹,

Fagan²,

Lovander³

et al. 2014

Developmental Biology

Self Cite

122

110

View full text Add to dashboard Cite

show abstract

“…Throughout Drosophila oogenesis, actin plays a role in tissue morphogenesis, signaling cascades, localization of mRNAs, and maintaining the integrity of the cortex (Spracklen, Kelpsch, Chen, Spracklen, & Tootle, 2014;Wang & Riechmann, 2007;Weil, Parton, Davis, & Gavis, 2008). To establish the distribution of the actin cytoskeleton before egg activation in the mature oocyte, we used the actin indicator F-tractin.…”

Section: Actin Reorganizes and Is More Dynamic During Drosophila Egmentioning

confidence: 99%

A calcium‐mediated actin redistribution at egg activation in Drosophila

York-Andersen

Wood

et al. 2019

Molecular Reproduction Devel

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Egg activation is the essential process in which mature oocytes gain the competency to proceed into embryonic development. Many events of egg activation are conserved, including an initial rise of intracellular calcium. In some species, such as echinoderms and mammals, changes in the actin cytoskeleton occur around the time of fertilization and egg activation. However, the interplay between calcium and actin during egg activation remains unclear. Here, we use imaging, genetics, pharmacological treatment, and physical manipulation to elucidate the relationship between calcium and actin in living Drosophila eggs. We show that, before egg activation, actin is smoothly distributed between ridges in the cortex of the dehydrated mature oocytes. At the onset of egg activation, we observe actin spreading out as the egg swells though the intake of fluid. We show that a relaxed actin cytoskeleton is required for the intracellular rise of calcium to initiate and propagate. Once the swelling is complete and the calcium wave is traversing the egg, it leads to a reorganization of actin in a wavelike manner. After the calcium wave, the actin cytoskeleton has an even distribution of foci at the cortex. Together, our data show that calcium resets the actin cytoskeleton at egg activation, a model that we propose to be likely conserved in other species.

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An ovary‐specific mucin is associated with choriogenesis mediated by prostaglandin signaling in Spodoptera exigua

Ahmed

Seo

Kim

2020

Arch Insect Biochem Physiol

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Polytrophic ovarioles of Spodoptera exigua, a lepidopteran insect, begins with the development of oocytes and differentiation of nurse cells followed by vitellogenesis and choriogenesis. Compared with previtellogenic and vitellogenic developments, choriogenesis has not been clearly understood yet in endocrine control. This study investigated the expression and function of a mucin-like structural protein of S. exigua called Se-Mucin1 in choriogenesis. It was highly expressed in ovarioles containing chorionated oocytes. The expression level of Se-Mucin1 was increased during adult stage as early as 18 h after adult emergence, reaching the maximal level at 24 h and later. Interestingly, DNA amount of Se-Mucin1 was increased by almost four folds during early adult stage while other genes (hexokinase and glyceraldehyde-3-phosphate dehydrogenase) not directly associated with chorion formation did not show genomic DNA increase, suggesting specific gene amplification of Se-Mucin1. RNA interference (RNAi) suppressed Se-Mucin1 expression by injecting 1 μg of double-strand RNA to teneral females (<5 h after emergence), which exhibited significantly impaired fecundity and egg hatching rate. Eggs laid by RNAi-treated females were malformed in eggshell structures with loss of mesh-like fibers. Treatment with aspirin, a prostaglandin (PG) biosynthesis inhibitor, suppressed the induction of Se-Mucin1 expression during early adult stage and impaired

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Prostaglandins temporally regulate cytoplasmic actin bundle formation duringDrosophilaoogenesis

Cited by 48 publications

References 80 publications

The pros and cons of common actin labeling tools for visualizing actin dynamics during Drosophila oogenesis

The pros and cons of common actin labeling tools for visualizing actin dynamics during Drosophila oogenesis

A calcium‐mediated actin redistribution at egg activation in Drosophila

An ovary‐specific mucin is associated with choriogenesis mediated by prostaglandin signaling in Spodoptera exigua

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