Prostate-specific antigen

Stenman, Ulf‐Hǎkan; Leinonen, Jari; Zhang, Wanming; Finne, Patrik

doi:10.1006/scbi.1998.0086

Cited by 166 publications

(104 citation statements)

References 70 publications

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“…This is thought to result from differences in the types of PSA released into the circulation by benign and malignant prostatic cells. PSA secreted by PCa cells is thought to be more active enzymatically than that leaking from the benign prostate; thus, a larger proportion forms a complex with ␣ 1 -antichymotrypsin (6 ). PSA isolated from BPH nodule fluids has been shown to contain a high proportion of cleaved forms (7 ), and free PSA in serum from patients with BPH has also been shown to be more extensively cleaved than that in PCa serum (8 ).…”

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confidence: 99%

Immunopeptidometric Assay for Enzymatically Active Prostate-Specific Antigen

Zhu

Stenman

et al. 2004

Clinical Chemistry

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Background: Determinations of certain forms of prostate-specific antigen (PSA) have been shown to increase the specificity for prostate cancer (PCa). One such variant, proteolytically active PSA, is a potentially useful tumor marker, but it is not specifically recognized by antibodies. Using phage display libraries, we previously identified a "family" of peptides that bind specifically to active PSA. We used these to develop an immunopeptidometric assay (IPMA) that specifically detects this form of PSA. Methods: Microtitration plates coated with a PSA antibody were used to capture PSA, and a PSA-binding glutathione S-transferase (GST) fusion peptide was used as a tracer. Bound tracer was detected with an antibody to GST labeled with a europium chelate. PSA isoenzymes with high and low enzymatic activity were used to study binding specificity. Results: The IPMA detected enzymatically active PSA but not internally cleaved PSA and pro-PSA, which are enzymatically inactive. The assay detected 1-10% of free PSA in serum from PCa patients. Conclusions: Peptides identified by phage display can be used to develop assays with unique specificities for enzymatically active PSA. IPMA represents a new assay principle with wide potential utility.

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confidence: 99%

Immunopeptidometric Assay for Enzymatically Active Prostate-Specific Antigen

Zhu

Stenman

et al. 2004

Clinical Chemistry

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“…Prostate cancer can be diagnosed early in the natural history of the disease by the detection of an elevated serum PSA, before the tumor is palpable on examination or causes symptoms. Although PSA, combined with DRE, provided higher specificity for prostate cancer screening (5), the elevation of PSA beyond the normal range may include benign prostate hyperplasia, old age, prostate trauma and prostate cancer (6). Clinicians should be aware of the possibility of synchronous primary rectal and prostate carcinomas as the delay in diagnosis of the second primary tumor was frequently the result of omission and poor awareness of multiple primary tumors (7).…”

Section: Discussionmentioning

confidence: 99%

Synchronous primary carcinomas of the rectum and prostate: Report of three cases

et al. 2011

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Abstract. Multiple primary tumors (MPT) are a well-known phenomenon. Rapid advancements in diagnostics and therapeutics have contributed to a significant improvement in the survival rates of cancer patients, and also in an increase in the incidence of cases with multiple primary neoplasms, such as synchronous primary carcinomas of the rectum and prostate. We present a case history of a small number of male patients with synchronous primary carcinomas of the rectum and prostate. Two of the three cases were treated with lower anterior resection (LAR) and radical retropublic prostatectomy (RRP) during the same operation, and 1 case was treated with abdominoperineal resection (APR) and RRP during the same operation. No significant complications occurred during these operations. Our experience with these 3 cases of synchronous primary carcinomas of the rectum and prostate indicated that LAR or APR and RRP can safely be performed in a single operation.

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“…We next investigated detection of PSA, a widely used biomarker in patients with prostate cancer (24 ). Healthy individuals generally have low concentrations of PSA (Ͻ0.1 ng/mL), whereas patients with prostate cancer have concentrations Ͼ4.0 ng/mL; hence, clinical examination of prostate cancer requires detection of PSA in serum ranging from 0.1 to 10 ng/mL (25,26 ).…”

Section: Magnetically Prompted Rapid Sandwich Immunoassay Using Ff Beadsmentioning

confidence: 99%