Prostatic kallikrein: A new major dog allergen

Mattsson, Lars; Lundgren, T. S.; Everberg, Henrik; Larsson, Håkan; Lidholm, Jonas

doi:10.1016/j.jaci.2008.11.021

Cited by 134 publications

(115 citation statements)

References 24 publications

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“…In conclusion, the article by Basagana et al [1] has the merit of highlighting the role of Can f 5 as a sensitizing agent that may be prevalent in a considerable percentage of dogsensitized patients, thus confirming previous findings [12,13]. Essential information collected for the clinical history and further studies assessing the eventual ubiquity of Can f 5 could be useful when attempting to establish its value in real life.…”

Section: To the Editorsupporting

confidence: 71%

What Could the Role of Can f 5 Allergen Be in Dog- Sensitized Patients in “Real Life”?

Liccardi

Salzillo

Apicella

et al. 2017

J Investig Allergol Clin Immunol

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Section: To the Editorsupporting

confidence: 71%

What Could the Role of Can f 5 Allergen Be in Dog- Sensitized Patients in “Real Life”?

Liccardi

Salzillo

Apicella

et al. 2017

J Investig Allergol Clin Immunol

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“…dog serum albumin, is an important cross-reactive allergen, and depending on the tested population up to 35% of dog-allergic patients reacted against Can f 3 [8,9]. Other dog allergens have been identified only recently, and they include a Fel-d-1-like allergen [10] and prostatic kallikrein [11]. …”

Section: Introductionmentioning

confidence: 99%

Skin Prick Test Extracts for Dog Allergy Diagnosis Show Considerable Variations Regarding the Content of Major and Minor Dog Allergens

Curin¹,

Reininger²,

Swoboda³

et al. 2010

Int Arch Allergy Immunol

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Background: Commercial skin prick test (SPT) extracts used for the diagnosis of dog allergy are prepared by extracting allergens from natural sources, e.g. dog hair and dander. Due to different starting material and extraction methods used, it is likely that extracts differ regarding their allergen contents. Methods: The total protein content and composition of dog SPT extracts from 5 European manufacturers were compared by silver-stained SDS-PAGE. Specific antibody probes were generated to detect major and minor allergens in each extract by immunoblotting. Additionally, sera of patients suffering from dog allergy were used to detect dog allergens in SPT extracts. Results: SPT extracts showed a 20-fold variation regarding the total protein content. The contents of the major dog allergen Can f 1 and of Can f 2 varied considerably between the extracts. In one of the extracts, neither Can f 1 nor Can f 2 could be detected by immunoblotting. The contents of the minor dog allergen Can f 3, albumin, also showed great variability. In one of the dog SPT extracts, the presence of human serum albumin (HSA) was detected with HSA-specific antibodies. Conclusion: The observed variability of commercial dog SPT extracts regarding their allergen contents likely has a negative influence on the accuracy of diagnosis of dog allergy.

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“…The IgE-binding bands in dog prostate secretions were identified by mass spectrometry and turned out to match with the recently described Can f 5 (arginine esterase peptidase S1 family, kallikrein subfamily) [8]. In order to examine the relationship with human PSA, the 31-kDa band was electroeluted and used as inhibitor in IgE immunoblotting inhibition experiments.…”

Section: Resultsmentioning

confidence: 99%

“…The protein was shown to be present also in dog dander and bound IgE antibodies from 70% of sera of dog-allergic patients were tested in that study, so it was found to be a major dog allergen [8]. …”

Section: Introductionmentioning

confidence: 99%

Involvement of Can f 5 in a Case of Human Seminal Plasma Allergy

Basagaña

Bartolomé²,

Pastor-Vargas³

et al. 2012

Int Arch Allergy Immunol

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Background: The existence of IgE binding to dog dander extract without IgE antibodies against the described dog allergens (Can f 1, 2, 3 and 4) implies the presence of other dog allergens yet to be identified. Recently, an IgE-binding protein was isolated from dog urine and identified as prostatic kallikrein; it has been named Can f 5. Cross-reactivity between a dog dander allergen and human prostate-specific antigen (PSA) has been described. The aim of this study was to identify the dog dander allergen that presents cross-reactivity with PSA and demonstrate its clinical relevance in our patient with human seminal plasma allergy. Methods: SDS-PAGE immunoblotting and inhibition tests were performed. Mass spectrometry was carried out to identify the protein involved in the allergy reactions. Results: SDS-PAGE immunoblotting-inhibition with an IgE-binding protein from dog prostatic secretion showed total IgE binding inhibition to a 28-kDa IgE-reactive band identified as PSA. The electroeluted protein from dog prostatic secretion was identified by mass spectrometry as Can f 5. IgE immunoblotting of human seminal plasma incubated with the serum of the patient revealed two IgE-binding bands (28 and 32.7 kDa). Both SDS-PAGE immunoblotting inhibition assays, with human seminal plasma or purified PSA in solid phase, showed complete IgE binding inhibition when the serum of the anaphylactic patient was preincubated with dog dander extract or recombinant Can f 5. Conclusions: The dog dander allergen that shows cross-reactivity with human PSA has been characterized and turns out to be the recently described Can f 5. We demonstrated the clinical relevance of this cross-reactivity in a patient.

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Prostatic kallikrein: A new major dog allergen

Cited by 134 publications

References 24 publications

What Could the Role of Can f 5 Allergen Be in Dog- Sensitized Patients in “Real Life”?

What Could the Role of Can f 5 Allergen Be in Dog- Sensitized Patients in “Real Life”?

Skin Prick Test Extracts for Dog Allergy Diagnosis Show Considerable Variations Regarding the Content of Major and Minor Dog Allergens

Involvement of Can f 5 in a Case of Human Seminal Plasma Allergy

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