Prostatitis

Nickel, J. Curtis

doi:10.5489/cuaj.686

Cited by 44 publications

(37 citation statements)

References 17 publications

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“…However, comprehensive knowledge about the microbial composition behind the inflammatory environment in BPH and prostatitis and progression of PC is understudied. Prostatitis is well categorized into different subtypes, considering the factors and the level of inflammation involved 11,12 ; however, the origin and factors involved in the development of BPH associated inflammation are not clear.…”

Section: Introductionmentioning

confidence: 99%

Escherichia coli, a common constituent of benign prostate hyperplasia‐associated microbiota induces inflammation and DNA damage in prostate epithelial cells

Jain

Samal²,

Das

et al. 2020

The Prostate

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Background The role of microbiota in the pathophysiology of benign prostate hyperplasia (BPH), especially in creating an inflammatory milieu may not be avoided. The major objectives of this study were to investigate the microbial composition of BPH tissues, its association with inflammation and check the effect of clinically isolated bacteria on prostate epithelial cells. Methods The study includes 36 patients with a pathological diagnosis of BPH. Following strict aseptic measures, tissues were collected after transurethral resection of prostate, multiple pieces of the resected tissues were subjected to histopathological analysis, bacterial culture and genomic DNA extraction. Microbial composition was analyzed by culture and/or next‐generation sequencing methods. Annotation of operational taxonomy unit has been done with an in‐house algorithm. The extent of inflammation was scored through histological evaluation of tissue sections. The effect of clinical isolates on nuclear factor‐κB (NF‐κB) activity and induction of DNA‐damage in the prostate epithelial cells were evaluated. Results Histopathological analysis of the BPH tissues showed the presence of inflammation in almost all the tissues with a varied level at different regions of the same tissue section and the level of overall inflammation was different from patients to patients. Microbial culture of tissue samples showed the presence of live bacteria in 55.5% (20 out of 36) of the patient tissues. Majority of the isolates were coagulase‐positive Staphylococcus, E. coli and Micrococcus spp. Further, V3 16S rRNA sequencing of the DNA isolated from BPH tissues showed the presence of multiple bacteria and the most common phylum in the BPH tissues were found to be Proteobacteria, Actinobacteria, Firmicutes, and Bacteroidetes. The E. coli, isolated from one of the tissue was able to activate NF‐κB and induce DNA damage in prostate epithelial cells. Phospho‐histone γH2A.X staining confirmed the presence of cells with damaged DNA lesion in BPH tissues and also correlated with the severity of inflammation. Conclusion Our study has shown that the BPH tissues do have a divergent microbial composition including the commonly found E. coli (phylum Proteobacteria), and these bacteria might contribute to the BPH‐associated inflammation and/or tissue damage. The BPH‐associated E. coli induced NF‐κB signaling and DNA damage in prostate epithelial cells in vitro.

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Section: Introductionmentioning

confidence: 99%

Escherichia coli, a common constituent of benign prostate hyperplasia‐associated microbiota induces inflammation and DNA damage in prostate epithelial cells

Jain

Samal²,

Das

et al. 2020

The Prostate

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“…Epididymitis occurs in approximately 1% of men and is the most common cause of scrotal pain. 43,44 It occurs when bacteria travels from urine back into the prostate or from retrograde ejaculation, causing diffuse inflammation. 45 Patients commonly present with unilateral testicular pain, swelling and inflammation, flank pain or lower abdominal discomfort, and fever.…”

Section: Epididymitismentioning

confidence: 99%

“…Urinary tract abnormalities, catheter use, and bicycle or motorcycle riding are risk factors. 43,44,46 The most concerning complication is infertility and chronic pain, although testicular abscess and sepsis can occur as well. 1,43,44,46 Diagnosis is based on clinical findings and objective data.…”

Section: Epididymitismentioning

confidence: 99%

Sexually Transmitted Infection Review for the Acute Care Pharmacist

Mah

Birmingham

Treu

et al. 2018

Journal of Pharmacy Practice

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“…В то же время длительное применение некоторых фармакологических препаратов может вызвать ряд побочных эффектов: α 1 -адреноблокаторы -гипотензию или ретроградную эякуляцию, ингибиторы 5α-редуктазы -исчезновение либидо или эректильную дисфункцию, нестероидные противовоспалительные препараты -диспепсию, пептические язвы, повышение риска сердечно-сосудистых осложнений и т. д. [7,[19][20][21][22][23][24].…”

Section: Introductionunclassified