Protaetia brevitarsis Extract Attenuates RANKL-Induced Osteoclastogenesis by Inhibiting the JNK/NF-κB/PLCγ2 Signaling Pathway

Jang, Hye-Yeon; Kim, Jeong-Mi; Kim, Jong‐Suk; Kim, Byeong-Soo; Lee, Young‐Rae; Bae, Jun Sang

doi:10.3390/nu15143193

Cited by 3 publications

(3 citation statements)

References 58 publications

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“…Differently from PLCγ2, PLCγ1 shRNA also inhibits osteoclast differentiation by suppressing CSF-1-dependent proliferation and β-catenin/cyclinD1 levels [168]. In addition, the abil-ity of PLCγ2 to regulate NFATc1 expression was positively regulated by JNK/NF-κB, whereas the ability of PLCγ1 to regulate NFATc1 expression was positively regulated by TLR4/TRAF6 [169]. Collectively, PLCγ1 and PLCγ2 are involved in osteoclastogenesis by regulating the expression of the key transcription factor NFATc1.…”

Section: Plc-gammamentioning

confidence: 96%

Role and Regulation of Transcription Factors in Osteoclastogenesis

Jiang,

Xia,

Qiao

et al. 2023

IJMS

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Bones serve mechanical and defensive functions, as well as regulating the balance of calcium ions and housing bone marrow.. The qualities of bones do not remain constant. Instead, they fluctuate throughout life, with functions increasing in some situations while deteriorating in others. The synchronization of osteoblast-mediated bone formation and osteoclast-mediated bone resorption is critical for maintaining bone mass and microstructure integrity in a steady state. This equilibrium, however, can be disrupted by a variety of bone pathologies. Excessive osteoclast differentiation can result in osteoporosis, Paget’s disease, osteolytic bone metastases, and rheumatoid arthritis, all of which can adversely affect people’s health. Osteoclast differentiation is regulated by transcription factors NFATc1, MITF, C/EBPα, PU.1, NF-κB, and c-Fos. The transcriptional activity of osteoclasts is largely influenced by developmental and environmental signals with the involvement of co-factors, RNAs, epigenetics, systemic factors, and the microenvironment. In this paper, we review these themes in regard to transcriptional regulation in osteoclastogenesis.

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Section: Plc-gammamentioning

confidence: 96%

Role and Regulation of Transcription Factors in Osteoclastogenesis

Jiang,

Xia,

Qiao

et al. 2023

IJMS

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“…The increasing interest in edible insects has led to the establishment of using insects in clinical applications based on their recognized pharmacological activities. A recent study reported that substances extracted from Protaetia brevitarsis seulensis (PBS) and peptides from its larvae exhibit various bioactivities, including anti-inflammatory, anticancer, and antimicrobial activities (Jang et al 2023;Yoon et al 2003). However, few studies have investigated the in vivo efficacy of these components in improving blood circulation.…”

Section: Introductionmentioning

confidence: 99%

“…A recent study reported that substances extracted from Protaetia brevitarsis seulensis (PBS) and peptides from its larvae exhibit various bioactivities, including anti‐inflammatory, anticancer, and antimicrobial activities (Jang et al . 2023; Yoon et al . 2003).…”

Section: Introductionmentioning

confidence: 99%

In vivo efficacy evaluation of white‐spotted flower chafer (Protaetia brevitarsis seulensis) extract for improving blood circulation

Son,

Chung

2024

Entomological Research

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Recent studies have suggested the use of insects to enhance various bioactivities in the treatment of many diseases or as alternative sources of nutrients. White‐spotted flower chafer (Protaetia brevitarsis seulensis; PBS) extract, which is used for medicinal purposes, appears to be effective in improving blood circulation. However, few studies have investigated the efficacy of PBS extract in improving blood circulation. Here, we investigated the effects of PBS extract on serum cholesterol metabolites (total cholesterol, high‐density lipoprotein cholesterol, and low‐density lipoprotein cholesterol) and anticoagulant activities (prothrombin time [PT], activated partial thromboplastin time [aPTT], and fibrinogen [Fib]) in very‐high‐fat diet (VHFD)‐induced hyperlipidemic rats. The experimental groups comprised a control group that was not treated with PBS and five PBS treatment groups (50, 100, 150, 200, and 250 mg/kg) in a randomized complete block design. In the efficacy evaluation for health functionality to reduce cholesterol levels, no beneficial effects on serum cholesterol metabolites were observed in the control and PBS treatment groups. In addition, the cholesterol data indicated that the effect of the PBS extract was not related to antioxidant activity. Moreover, the PT, aPPT, and Fib values were prolonged by increasing the PBS extract concentration, and PBS extract concentrations of 150–250 mg/kg were recommended for the VHFD model based on the anticoagulant activity. In conclusion, the PBS extract can be used in a potential therapeutic strategy for improving anticoagulant activity rather than improving the serum cholesterol profile.

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Protaetia brevitarsis larvae extract protects against lipopolysaccharides-induced ferroptosis and inflammation by inhibiting acid sphingomyelinase

Park,

Oh,

Kim

2024

Nutr Res Pract

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BACKGROUND/OBJECTIVES Inflammation and ferroptosis are implicated in various diseases and lipopolysaccharides (LPS) have been linked with these disorders. Recently, many edible insects, such as Gryllus bimaculatus, Protaetia brevitarsis larvae (PB) and Tenebrio molitor larvae, have been recommended as alternative foods because they contain lots of nutritional sources. In this study, we explored the potential of PB extract in preventing LPS-induced inflammation and ferroptosis in Hep3B cells. MATERIALS/METHODS PB powder was extracted using 70% ethanol and applied to Hep3B cells. Co-treatment with LPS was conducted to induce ferroptosis and inflammation. The anti-inflammatory and anti-ferroptosis mechanisms of the PB extract were confirmed using Western blot, enzyme-linked immunosorbent assay, and real-time polymerase chain reaction analysis. RESULTS PB extract effectively prevented LPS-induced cell death and restored LPS-induced inflammatory cytokine production, NF-κB signaling, endoplasmic reticulum (ER) stress and ferroptosis. Interestingly, PB extract reduced LPS-induced ceramide increase and acid sphingomyelinase (ASMase) expression. The use of the ASMase inhibitor, desipramine, also demonstrated a reduction in these pathways, highlighting the pivotal role of ASMase in inflammation and ferroptosis. Treatment with each inhibitor revealed that ferroptosis causes ER stress and that NF-κB and MAP kinase pathways are involved in inflammation. CONCLUSION PB emerges as a potential functional food with inhibitory effects on LPS-induced inflammation and ferroptosis, making it a promising candidate for nutritional interventions.

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Protaetia brevitarsis Extract Attenuates RANKL-Induced Osteoclastogenesis by Inhibiting the JNK/NF-κB/PLCγ2 Signaling Pathway

Cited by 3 publications

References 58 publications

Role and Regulation of Transcription Factors in Osteoclastogenesis

Role and Regulation of Transcription Factors in Osteoclastogenesis

In vivo efficacy evaluation of white‐spotted flower chafer (Protaetia brevitarsis seulensis) extract for improving blood circulation

Protaetia brevitarsis larvae extract protects against lipopolysaccharides-induced ferroptosis and inflammation by inhibiting acid sphingomyelinase

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