Protease activity and protein profile in milk from healthy dairy cows and cows with different types of mastitis

Caggiano, Nicolás; Smirnoff, A. Lorenzo; Bottini, J.M.; Simone, Emilio A. De

doi:10.1016/j.idairyj.2018.07.020

Cited by 11 publications

(11 citation statements)

References 32 publications

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“…Results demonstrated no significant difference in the concentration of fat and protein in subclinical and clinical mastitis milk. This is in agreement with several works (Malek dos Reis et al ., 2013; Gonçalves et al ., 2016; Caggiano et al ., 2019). On the other hand, a significant decrease in lactose content was detected in subclinical and clinical mastitis milk ( P < 0.05).…”

Section: Resultsmentioning

confidence: 99%

“…3, Table S1). This corresponds with previous studies reported that the total amino acid content of high SCC milk increased significantly, with a particular attention in the content of branched‐chain amino acids and aromatic amino acids (Melzer et al ., 2013; Xi et al ., 2017; Ianni et al ., 2019) due to a high activity of proteolytic enzymes, especially plasmin, polymorphonuclear (PMN)‐derived proteases, as well as bacterial proteases in milk (Caggiano et al ., 2019).…”

Section: Resultsmentioning

confidence: 99%

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Comparative metabolite profiling of raw milk from subclinical and clinical mastitis cows using ¹H‐NMR combined with chemometric analysis

Luangwilai

Duangmal

Chantaprasarn

et al. 2020

Int J of Food Sci Tech

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The objectives of this study were to characterise and compare non-volatile metabolite profiles of raw milk from healthy, subclinical and clinical mastitis cows using a non-targeted proton nuclear magnetic resonance (1 H-NMR) metabolomic approach. A total of 46 metabolites were identified. Multivariate analysis allowed discrimination of milk from healthy and mastitis cows according to their 1 H-NMR metabolite profiles. Significant increases in acetate, formate, lactate, benzoate, hippurate, b-hydroxybutyrate, valerate, alanine, histidine, isoleucine, leucine, N-acetylamino acid, phenylalanine, threonine and valine content were associated with extremely high somatic cell count and clinical mastitis status of the cows. Furthermore, significant rises in hippurate, valerate, N-acetylglucosamine, histidine, isoleucine and leucine content could be used as potential biomarker for indicating subclinical mastitis infection of the udders. Beside the existing biomarkers for diagnosing mastitis in literatures, we found additional candidate metabolites, such as alanine, valerate and N-acetylglucosamine, in correlation with mastitis status of Thai crossbred Holstein dairy cows.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Comparative metabolite profiling of raw milk from subclinical and clinical mastitis cows using ¹H‐NMR combined with chemometric analysis

Luangwilai

Duangmal

Chantaprasarn

et al. 2020

Int J of Food Sci Tech

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“…This family of proteases have five subfamilies, of which MMP9 is one of the most studied. It has been shown that the levels of expression of MMP9 in cases of bovine E. coli mastitis (Caggiano et al, 2019) and in goat mammary gland epithelial cells with S. aureus infection (Li et al, 2016) were increased. Considering all the results, MMP9 may be a good candidate for biomarker of E. coli mastitis diagnosis.…”

Section: Degsmentioning

confidence: 99%

Identification of Key Candidate Genes in Dairy Cow in Response to Escherichia coli Mastitis by Bioinformatical Analysis

Chen²,

Chen

2019

Front. Genet.

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At present, bovine mastitis is one of the most costly diseases affecting animal health and welfare. Escherichia coli (E. coli) is considered to be one of the main pathogens causing mastitis with clinical signs in dairy cattle. However, the cure rate of E. coli mastitis is low, and the pathogenesis of E. coli mastitis is not completely known. In order to develop new strategies for the rapid detection of E. coli mastitis, a comprehensive molecular investigation of E. coli mastitis is necessary. Hence, this study integrated three microarray data sets to identify the potential key candidate genes in dairy cow in response to E. coli mastitis. Differentially expressed genes (DEGs) were screened in mammary gland tissues with live E. coli infection. Furthermore, the pathways enrichment of DEGs were analyzed, and the protein-protein interaction (PPI) network was performed. In total, 105 shared DEGs were identified from the three data sets. The DEGs were significantly enriched in biological processes mainly involved in immunity. The PPI network of DEGs was constructed with 102 nodes and 546 edges. The module with the highest score through MCODE analysis was filtered from PPI; 18 central node genes were identified. However, in addition to immunerelated pathways, some of the 18 DEGs were involved in signaling pathways triggered by other diseases. Considering the specificity of biomarkers for rapid detection, IL8RB, CXCL6, and MMP9 were identified as the most potential biomarker for E. coli mastitis. In conclusion, the novel DEGs and pathways identified in this study can help to improve the diagnosis and treatment strategies for E. coli mastitis in cattle.

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“…The use of MMP and TIMP plasma levels as biomarkers in different clinical settings is increasing due to their diagnostic and prognostic value in human medicine (23,27). Currently, only a few studies have been focused on plasma or milk levels of MMP (21,28), while the plasma level of TIMPs in cattle is lack of relevant research. The present study showed that the pro-MMP9 activity increased in plasma from 36 to 60 h in the OF-induced group.…”

Section: Discussionmentioning

confidence: 99%

Change in Proteolytic Profile in Heifers After Oligofructose Overload

Zheng

Ding

et al. 2020

Front. Vet. Sci.

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Laminitis in cattle is an important underlying cause of lameness, which leads to a significant reduction in economic and animal welfare. Nevertheless, the disordered pathological processes of laminitis remain unclear. Several proteinases are probably involved in the disorder of basement membrane (BM) metabolism in laminitis, for instance, matrix metalloproteinases (MMPs), neutrophil elastase (NE), and myeloperoxidase (MPO). This study aimed to investigate the change in proteolytic profile in circulating and lamellar tissues using an oligofructose (OF) overload-induced laminitis model in heifers. Twelve clinically healthy and nonlame Chinese Holstein heifers were recruited and randomly divided into two groups: OF-induced and control (CON). The OF-induced heifers group (n = 6) was administered 17 g/kg of body weight (BW) of OF dissolved in 2 L/100 kg of BW of tap water via the oral-rumen tube. The CON group (n = 6) was given an equal volume of tap water. The plasma samples were collected 0, 6, 12, 18, 24, 36, 48, 60, and 72 h after administration, and the lamellar samples were collected 72 h after euthanasia. The plasma samples were analyzed by zymography and reverse zymography. Histological examination, zymography, reverse zymography, and Western blot of lamellar samples were conducted. In the plasma of the OF-induced group, the pro-MMP9 activity increased from 36 h (P < 0.001) to 60 h (P < 0.05). Moreover, the plasma tissue inhibitors of metalloproteinase 1 (TIMP1) activity decreased after 18 h (P < 0.05), while the ratio of pro-MMP9 to TIMP1 and TIMP2 increased after 18 h (P < 0.001) and 48 h (P < 0.05), respectively. The act-MMP2, pro-MMP9, and act-MMP9 activities increased in the lamellar tissue of the OF-induced group compared with the CON group (P < 0.05). In addition, the expression of lamellar NE protein was higher in the OF-induced group (P < 0.01), while no change was found in lamellar MPO protein compared with the CON group. In conclusion, increased pro-MMP9 combined with decreased TIMP1 activity in the circulation might have caused the activation of blood neutrophils, while the activation of proteolytic enzymes in lamellar tissue probably led to the dysfunction of BM in the OF-induced group.

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Protease activity and protein profile in milk from healthy dairy cows and cows with different types of mastitis

Cited by 11 publications

References 32 publications

Comparative metabolite profiling of raw milk from subclinical and clinical mastitis cows using ¹H‐NMR combined with chemometric analysis

Comparative metabolite profiling of raw milk from subclinical and clinical mastitis cows using ¹H‐NMR combined with chemometric analysis

Identification of Key Candidate Genes in Dairy Cow in Response to Escherichia coli Mastitis by Bioinformatical Analysis

Change in Proteolytic Profile in Heifers After Oligofructose Overload

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Protease activity and protein profile in milk from healthy dairy cows and cows with different types of mastitis

Cited by 11 publications

References 32 publications

Comparative metabolite profiling of raw milk from subclinical and clinical mastitis cows using 1H‐NMR combined with chemometric analysis

Comparative metabolite profiling of raw milk from subclinical and clinical mastitis cows using 1H‐NMR combined with chemometric analysis

Identification of Key Candidate Genes in Dairy Cow in Response to Escherichia coli Mastitis by Bioinformatical Analysis

Change in Proteolytic Profile in Heifers After Oligofructose Overload

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Comparative metabolite profiling of raw milk from subclinical and clinical mastitis cows using ¹H‐NMR combined with chemometric analysis

Comparative metabolite profiling of raw milk from subclinical and clinical mastitis cows using ¹H‐NMR combined with chemometric analysis